陆地棉ZF-HD蛋白的全基因组分析

ZF-HD(Zinc finger-homeodomain)蛋白属于同源异型盒蛋白家族,在动植物发育过程中起重要的作用。利用生物信息学的手段,研究陆地棉标准系TM-1基因组中ZF-HD蛋白的数目、亚细胞定位、染色体分布、基序、进化关系和组织表达情况。TM-1中共有35个Gh ZHD蛋白成员,且大部分成员定位到细胞核内;分布在20条染色体和2条Scaffold上,且具有11对直系同源基因;进化树分析表明,TM-1基因组中的ZF-HD蛋白大致分为6个小组,每个小组成员间具有类似的基序类型和排列顺序;大部分Gh ZHD基因在胚珠和纤维组织中表达,还有部分基因在根、茎、花、蕾和分生区中表达,在叶和愈伤组织中表达的基因最少。     

大凌河流域生态综合治理项目效益分析

葫芦岛市建昌县2014年国家农业综合开发大凌河流域生态综合治理项目分布在建昌县新开岭乡新开岭村、贺杖子乡碾房村、素珠营子乡梁杖子村.通过该项目治理后,生态环境得到明显改善,经济效益十分显著,社会效益巨大,促进了区域经济社会发展.同时工程建设也起到了科技示范作用,为推动生态环境建设奠定了坚实基础保障.     

柴达木盆地梭梭LEA基因的扩增及序列分析

为发掘梭梭(Haloxylon ammodendron)抗逆相关基因,以柴达木盆地梭梭的同化枝为材料,扩增其LEA基因并进行了序列测定与分析。结果表明,所得柴达木盆地梭梭的LEA基因碱基数为234 bp,其中A碱基70个,T碱基52个,G碱基59个,C碱基53个;氨基酸序列分析表明,所得LEA基因片段编码78个氨基酸,其中强碱性氨基酸有12个,强酸性氨基酸有6个,疏水性氨基酸有34个,亲水性氨基酸有26个。     

枣抑制消减cDNA文库构建与分析

枣树具有独特的开花结果特性,为获得枣花发育基因,以金丝4号枣不同发育时期的花蕾和同时期的叶片cDNA互为试验方和驱动方,利用抑制消减杂交技术分别构建了枣花和枣叶抑制消减杂交cDNA文库(SSH文库)。枣花和枣叶SSH文库重组率分别是92.05%和90.26%,插入片段长度均介于200~2 000 bp之间,主要集中于1 000 bp左右。分别从枣花和枣叶SSH文库中随机挑选1 000个阳性克隆进行DNA测序、组装拼接,结果显示枣花SSH文库中获得96条unigene,其中contigs 43个,singletons 53个,96条unigene预测得到73个ORF,经过同源比对分析获得有注释的序列20条,按功能分为7类;枣叶SSH文库中获得86条unigene,其中contigs 45个,singletons 41个,86条unigene预测得到70个ORF,经过同源比对分析获得有23条注释的序列,按功能分为6类。     

基于GIS的村级尺度统计数据空间化方法与时序分析初探

选择重庆市巴南区石龙镇大连村、大兴村、白马村、金星村、合路村、柏树村、大桥村、大园村和中伦村9个行政村作为研究区,将研究区的遥感影像、数字高程模型、行政界线等数据的空间参考坐标系统一为1980西安坐标系,选择2006-2012年研究区的农用肥施用量数据作为村级尺度统计数据空间化方法研究的研究数据,利用专家打分法对不同土地利用类型与农用肥施用之间的相关性进行打分,分值范围是0~1分。结合村级行政界线、行政村面积、农用肥施用量、土地利用数据和专家打分结果,构建农用肥施用数据空间化模型,分别生成2006-2012年村级农用肥施用数据空间化分布栅格数据,栅格尺度选择1、10、100和200 m这4种,并对栅格化结果进行对比分析。同时,对2006-2012年的农用肥施用空间分布数据进行时序分析,获取农用肥施用量的动态变化趋势。通过研究得到以下结论:1村级农用肥施用量数据空间化尺度与土地利用数据的类型及空间分辨率有关;2研究区各行政村农用肥施用量在2006-2012年期间总体变化趋于平稳。本研究对于实现村级统计数据空间化方法具有一定的参考意义,而空间化后的村级统计数据对于进行村镇区域规划、土地整改、生态环境保护等具有重要指导意义。     

Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes

The present study was aimed to explore the potential role of Toll-like receptors (TLRs) on the mink immunity, and accumulate alternative genetic material for the mink breeding for disease-resistant.Four pairs of primers were designed according to ferret TLR sequences in GenBank to obtain the sequences of TLR genes (TLR4, TLR6, TLR7 and TLR8) of mink and then performed bioinformatics analysis of these sequences.In addition, the expression of TLR4 and TLR7 genes in various tissues in young and adult minks were analyzed by RT-PCR. Sequence homology analysis showed that mink had higher homology with carnivora (ferrets, polar bear, panda, walrus, seals, dog, tiger and cat) which had the nearest perimeter in the phylogenetic tree.Tissue expression analysis showed that TLR4 and TLR7 were widely and differently expressed in variety tissues of mink.Interestingly, the expression of TLR4 and TLR7 in young mink were much higher than that in adult mink.     

Cloning and Sequence Analysis of Complete Genome of Porcine Circovirus Type 2 from Liaoning Province

It was aimed to lay a foundation for epidemiological survey of porcine circovirus type 2(PCV2) in Liaoning province and selection of highly homologous strain inactivated vaccine.Some pigs suspected of having postweaning multisystemic wasting syndrome (PMWS) from Shenyang,Chaoyang,Tieling,Dalian and other places in Liaoning province,were detected by PCR method,then the whole genome of 10 positive samples were cloned and sequenced.The result showed that all of them were virulent strains,9 strains were 1767 bp and 1 strain was 1768 bp,and there was a T base additon at 1039 bp.The nucleotide homology was 95.1% to 98.5% with HM038034 and JQ692110.In the phylogenetic tree,JHZ2 was PCV2a type,the other 9 strains were PCV2b type,DLS38,LYD32 and SYX7 were in the same branch and same onset time.The amino acid homology of ORF2 was 89.6% to 100.0%,there were 35 mutation points,large degree of variation,the only glycosylation site (NYS) was conserved.10 strains did not change significantly,mutants were not due to PCVD,PCV2b was the predominant type,and PCV2 was related with seasonality.     

大兴安岭生态功能区建设的区划分析及“G＋X”管理模式构建

依据价值分析理念,对大兴安岭生态功能区进行了区划分析,并构建了大兴安岭生态功能区建设的＂G＋X＂管理模式。     

黄石市休闲农业发展的SWOT分析

采用SWOT分析法,对湖北省黄石市休闲农业发展中存在的优势、劣势、机遇和挑战进行了深入分析,并在此基础上构建了黄石市休闲农业发展的战略矩阵,旨在对黄石市休闲农业的发展提供参考.     

玉米粒重一致性QTL整合图谱的构建及元分析

为进一步研究候选基因发掘、克隆以及基因组结构,在生物信息学基础上,利用公共网络资源上已发表的149个玉米粒重相关QTL位点信息,初步探讨了玉米粒重"一致性"QTL发掘。结果表明:构建出一个包含了80个玉米粒重QTL位点的整合图谱,并在第7染色体上确定出一个"一致性"QTL,由标记bcd349和gpm760a界定,置信区间为463.29~505.50,间距42.21cM。