Identification of collembolan species is generally based on specific morphological characters, such as chaetotaxy and pigmentation pattern. However, some specimens do not match to described characters because these refer to adult specimens, often of one specific sex, or the characters are highly variable in adults (e.g. pigmentation, setae or furcal teeth). Isozymes have frequently assisted species discrimination, and also these may vary with developmental stage or environmental conditions. For identification of single species of the Isotoma viridis group, we present both direct sequencing of the cytochrome oxidase subunit II (COII) gene and a simple DNA-based molecular method.
Five PCR primers amplifying the COII region (717 bp) of the mitochondrial DNA were used. The sequences clearly separated the species I. viridis, I. riparia and I. anglicana, irrespective of colour varieties within the first species. DNA amplification products of different species can also be distinguished by digestion with restriction endonucleases, followed by gel electrophoresis for separation of fragments. This restriction fragment length polymorphism (RFLP), obtained after digestion with the endonucleases TaqI, VspI, MvaI and Bsp143I, revealed specific fragments that separated the three species from each other. Since restriction enzymes are sensitive to single base mutations, we suggest to use a combination of enzymes with at least two species-specific restriction sites when using the RFLP technique. For the I. viridis complex, VspI and Bsp143I appear to be an appropriate combination. 相似文献
为探讨绿洲区不同灌溉方式及灌溉量对苜蓿田土壤盐分空间分布及运移的影响,在苜蓿生长第2年设滴灌和漫灌2种灌溉方式,每种灌溉方式设3个灌溉梯度,对土壤电导率进行了测定与分析。结果表明,随生育进程的推进及灌水次数的增加,整个苜蓿生育期内土壤盐分含量呈波动式递减的变化趋势。滴灌方式下0~40 cm 土层、漫灌方式下0~30 cm 土层在灌溉后土壤处于脱盐状态;滴灌方式下在50~60 cm 土层、漫灌方式下在40~60 cm土层土壤处于积盐状态。在水平及垂直方向上,随灌水量的增加,2种灌溉方式下土壤盐分峰值位均呈下移趋势,灌溉后土壤盐分含量随土壤深度的增加而增大,土壤盐分主要集中于土壤40~60 cm 土层,且滴灌方式下的“驱盐”效果要好于漫灌。在考虑成本、产出等综合经济效益时,当灌水量达到某种定额(滴灌下3000 m3/hm2,苜蓿干草产量为1406 kg/667 m2,漫灌下5250 m3/hm2,其干草产量为1504 kg/667 m2)后即可达到最佳的“驱盐”及高产效果,在绿洲区苜蓿田过多的灌溉量只会导致水资源的更多浪费。对绿洲区盐渍化较重的农田进行灌溉时,应尽量选取合理的灌溉制度及适宜的灌水量,以达到土壤盐分淋洗和经济效益最大化的双赢。 相似文献