松根炭制维尼纶载体活性炭的研究

作为维尼纶合成中的载体活性炭，其强度和孔径分布要求十分严格，所以需要使用椰壳活性炭。本研究的目的就是制造一种活性炭来代替它。实验证明，松根炭制成的活性炭可以作为载体活性炭。其关键是粘结剂、辅助试剂和活化条件的选择。     

Real-time detection of <Emphasis Type="Italic">Phytophthora nicotianae</Emphasis> and <Emphasis Type="Italic">P. citrophthora</Emphasis>in citrus roots and soil

Two primers, specific for Phytophthora nicotianae (Pn6) and P. citrophthora (Pc2B), were modified to obtain Scorpion primers for real-time identification and detection of both pathogens in citrus nursery soils and roots. Multiplex PCR with dual-labelled fluorogenic probes allowed concurrent identification of both species ofPhytophthora among 150 fungal isolates, including 14 species of Phytophthora. Using P. nicotianaespecific primers a delayed and lower fluorescence increase was also obtained from P. cactorumDNA. However, in separate real-time amplifications, the aspecific increase of fluorescence from P. cactorum was avoided by increasing the annealing temperature. In multiplex PCR, with a series of 10-fold DNA dilutions, the detection limit was 10 pg l^-1 for P. nicotianaeand 100 pg l^–1 for P. citrophthora, whereas in separate reaction DNA up to 1 pg l^-1 was detected for both pathogens.Simple and rapid procedures for direct DNA extraction from soil and roots were utilised to yield DNA whose purity and quality was suitable for PCR assays. By combining these protocols with a double amplification (nested Scorpion-PCR) using primers Ph2-ITS4 amplifying DNA from the main Phytophthora species (first round) and PnB5-Pn6 Scorpion and Pc2B Scorpion-Pc7 (second round), it was possible to achieve real-time detection of P. nicotianaeand P. citrophthora from roots and soil. The degree of sensitivity was similar to that of traditional detection methods based on the use of selective media. The analyses of artificially and naturally infested soil showed a high and significant correlation between the concentration of pathogen propagules and the real-time PCR cycle threshold.     

Possible Root Infection of Cercospora beticola in Sugar Beet

A potential primary infection site of the foliar pathogen Cercospora beticola in sugar beet is described. Sugar beet seedlings of the susceptible cv. Auris were grown in a standard soil for 14 days. A monoconidial culture of a C. beticola isolate was grown to produce conidia. In experiment 1, roots were immersed in a conidial suspension of isolate code IRS 00-4, or in tap water (control), for 2 days. After incubation seedlings were potted in a peat – fine river sand mixture and placed at low relative humidity (RH) (<80%) or high RH (100%). Twelve days after infection, seedlings at high RH showed more disease incidence (90%) than seedlings grown at low RH (disease incidence = 25%), whereas no disease symptoms developed in the control seedlings. Cercospora leaf spots (CLSs) developed on the cotyledons, leaves, petioles and stems of the seedlings. In experiment 2, roots were immersed in a conidial suspension of isolate code IRS 00-2 for 5 h. Thirty-four days after infection at high RH, 100% disease incidence was observed in the treated seedlings and one CLS in the control treatment. First indications of leaf spot development were observed as reddish purple discolouration of individual parenchymatic cells. Because splash dispersal and symptoms due to infested soil were excluded, we showed that it is possible to obtain CLS symptoms in sugar beet seedlings when their roots were immersed in conidial suspensions of C. beticola, thus demonstrating that roots can be a primary infection site.     

浅谈大树移植技术

移植大树对于加快城市绿化、公路、铁路绿色通道建设等具有重要作用。大树移植的成功与否,除了起苗、搬运、定植、栽后管理4个基本环节外,提出了“断根缩坨”促发须根提高大树移栽成活率的新技术。     

高温胁迫下高羊茅草坪草生理特性研究

从美国引进的39个高羊茅草坪草种分成了5个生态类型,在1999—2000年从每类中找出一个具有典型性状的草种,测定其周年草坪坪质表现、根系活力、叶片可溶性糖含量变化,同时测定各类草种在高温胁迫下保护酶活性与质膜透性的变化。结果表明,根系活力及可溶性糖含量在一定程度上影响着草坪坪质的周年变化。同时,草坪草叶片MDA含量的增长率和草坪草叶片的质膜透性增长率具有显著的正相关效应。在40℃高温胁迫下,草坪草叶片SOD与POD相对活性和MDA含量的增长率及PMP增长率具有显著的负相关效应,5个草种对高温的抗性能力大小顺序是:Gooden>Regi ment>Tomahawk>SR-8210>Brandy。在此基础上,最后对草坪草抗性的准确快速检测方法进行了讨论。     

施用胂·锌·福美双对西洋参及其栽培土壤中砷残留量的影响

选择3年生西洋参进行田间试验,设计胂·锌·福美双两个施用量以400～600倍药液均匀喷施全株,处理2年后,测定田间土壤样品及西洋参根中砷含量.西洋参根中砷含量与胂·锌·福美双的使用量有显著相关关系,与对照相比,每年施用3～4次胂·锌·福美双,砷含量增加64.52％～101.68％,但低于目前中国药典的相关规定(2mg/kg).胂·锌·福美双的施用与土壤砷含量无显著相关关系(R2 =0.1890).在相同施用量情况下,随着有机质含量的增加土壤砷含量有所增加.含砷类农药的施用有可能增加西洋参中砷的残留,应尽量减少高含量重金属杀菌剂的应用并关注有关肥料的使用.     

灌溉方式对温室樱桃番茄结果期根系特征与产量的影响

采用小区试验研究了不同灌溉方式对樱桃番茄结果期根系特性与产量的影响。试验设置了6个灌溉方式处理,即:处理Ⅰ,常规沟灌,种植行和操作行同时灌溉;处理Ⅱ,交替沟灌,种植行和操作行交替灌溉;处理Ⅲ,固定灌种植行;处理Ⅳ,固定灌操作行;处理Ⅴ,前期常规沟灌,结果期交替沟灌;处理Ⅵ,前期交替沟灌,结果期常规沟灌。结果表明,不同灌溉方式之间樱桃番茄根系的特征值差异达到显著水平,处理Ⅵ根体积显著高于其余5种处理,根系伤流量为22.37 mg/min,根系活力高达0.50 mg/(g.h)。灌溉方式对樱桃番茄单果重、产量有显著影响,前期交替沟灌结果期常规沟灌(处理Ⅵ)的单果重与产量均高于其他处理。因此,前期交替沟灌结果期常规沟灌有利于樱桃番茄根系的发育与产量的提高。     

华北土石山区四种常见乔木根系的形态研究

以华北土石山区四种常见乔木的根系为研究对象,采用全挖法,从根长、根径、土层深度和生物量等方面进行比较,并计算了四个树种随土壤深度变化的累积根系长度百分比,利用Sketchup Pro 8软件建立了各根系的形态模型。结果表明:四个树种根系长度差别较大,总长度大小顺序依次为白桦>落叶松>蒙古栎>油松;不同树种不同径级根的差别较大;各树种根系垂直分布范围集中在0～60cm的土层中,在60cm以下的土层中分布很少。研究中四个树种拟合的β值都在0.960-0.970之间,根系垂直分布方式都显示出深根型根系轮廓。用软件模拟出的根系形态较接近实际。     

列当杂草及其防除措施展望

根寄生杂草列当Orobanche spp.每株可产生大量细小的种子,易于传播,且在出苗前已经对寄主作物造成危害,使列当的防除成为一个世界性难题。本文详述了列当的生活史、为害现状以及防除措施,并突出了生物防除的生态效益与经济效益。基于＂诱捕＂作物及＂捕获＂作物诱导列当种子＂自杀发芽＂理论的防除机制,本文提出了新型可持续生态防除思想,为列当科杂草防除提供参考。     

Evaluation of the combination of 1,3-dichloropropene and dazomet as an efficient alternative to methyl bromide for cucumber production in China

BACKGROUND: The combination of 1,3‐dichloropropene (1,3‐D) and dazomet (DZ) offers a potential alternative to methyl bromide (MB) for soil disinfection. MB is scheduled to be withdrawn from routine use by 2015 in developing countries. Combination treatments of 1,3‐D + DZ were evaluated in a laboratory study and in two commercial cucumber fields. RESULTS: Laboratory studies found that nearly all of the tested combinations of 1,3‐D and DZ displayed positive synergistic activity on root‐knot nematodes (Meloidogyne spp.), two major soilborne fungi (Fusarium spp. and Phytophthora spp.) and the seeds of two major weed species (Digitaria sanguinalis and Abutilon theophrasti). Field trials revealed that the combination of 1,3‐D and DZ (at 10 + 25 g m^?2) successfully suppressed Meloidogyne spp. root galling, sharply reduced Fusarium spp. and Phytophthora spp. and maintained high cucumber yields. The combination treatment of 1,3‐D + DZ was more effective than 1,3‐D or DZ alone and provided results similar to methyl bromide with respect to pest control, plant mortality, plant height, yield and income. All of the treatments were significantly better than the non‐treated control. CONCLUSION: The results indicate that the tested combination of 1,3‐D and DZ offers an efficient alternative to methyl bromide for cucumber production. Copyright © 2012 Society of Chemical Industry