The role of genetic mechanisms of sex determination in the survival of small populations of Silene littorea: A reintroduction experiment

Loss of genetic variation in populations may have other effects than inbreeding depression and loss of adaptive potential. In the case of the numerous gynodioecious plant species having cytonuclear systems of sex determination with dominant nuclear restorers of the male function, reduced genetic variation and increased inbreeding would increase the proportion of non-restorer recessive genes found in homozygosity, and therefore that of female plants producing pistillate flowers. This could have opposite effects on the extinction risk of the population. Female plants produce no pollen and may cause pollen limitation, but at the same time they may reduce average homozygosity and inbreeding depression because they are forced outcrossers. We observed that inbreeding indeed increased the frequency of female plants and pistillate flowers in the population, and that this effect was accompanied by reductions in population recruitment independent of inbreeding depression and likely due to pollen limitation. These results suggest that dominant nuclear restorers of pollen production speed up the extinction of small populations of Silene littorea, and that sex determination mechanisms might be an important factor to consider in the conservation of many plant species.     

The food limitation hypothesis for juvenile marine fish

Coastal zones are productive areas that serve as nursery grounds for a large number of marine species. However, the processes involved in survival success during the juvenile phase are not well‐known. Some authors suggest that the availability of prey is important to support the production of pre‐recruit fish whose fitness is enhanced through optimal feeding conditions. Accordingly, recruitment is limited by the carrying capacity of the nursery habitat. In contrast, other authors state that the carrying capacity of the nursery grounds is not fully exploited, suggesting that there is no effect of food limitation. This study combines an overview of the literature, focused on flatfish that are especially dependent on coastal and estuarine nursery grounds, an extension to other marine fishes and a modelling approach on growth and survival of juvenile fish to explore the controversy of food limitation in their nursery grounds. We demonstrate that the relative lack of growth limitation observed for young marine fishes at the individual scale is related to an observational bias: fish have been affected by size‐selective mortality linked to food limitation, but only surviving individuals are observed. As the population is skewed towards the faster‐growing juveniles, the growth of survivors remains close to optimal, even when food resources are limited. Food limitation is of major influence in determining the carrying capacity of the nursery habitat. To sustain marine fish populations and related fisheries, management action is needed to protect coastal and estuarine areas and maintain or restore nursery productivity.     

Evaluation of a fish meal analogue as a replacement for fish meal in practical diets for Pacific white shrimp Litopenaeus vannamei

Three six‐week growth trials and a digestibility trial were conducted to evaluate a fish meal analogue (FMA) as a replacement for fish meal (FM) in shrimp feeds. Trials 1 and 2 evaluated and confirmed the potential of FMA supplementation (0, 48.5, 97, 145.5 and 194 g/kg) as a replacement for FM up to 200 g/kg without balancing for phosphorus (P) in practical diets for juvenile Pacific white shrimp L. vannamei. At the end of trial 1, shrimp offered diets containing 48.5 g/kg FMA exhibited significantly higher weight gain (WG) than those fed with the diet containing 145.5 g/kg FMA. At the end of trial 2, dietary FMA inclusion at 48.5 and 97 g/kg significantly improved WG and protein retention (PR), while reducing FCR and protein content of shrimp body compared to the diet containing 194 g/kg FMA. To determine whether P deficiency is the cause of reduced growth, the third trial was conducted utilizing equivalent diet but balanced for P. At the end of trial 3, shrimp fed diet containing 48.5 g/kg FMA+P showed significantly higher WG and PR than those fed diet containing 145.5 g/kg FMA+P. No decreasing trend of growth was detected in the diets containing FMA compared to the FM‐based diet. Apparent digestibility coefficients of dry matter, energy, protein and amino acids of FMA were determined using chromic oxide as an inert maker and the 70:30 replacement technique. The energy, protein and individual amino acid digestibility of FMA were significantly lower than those of soybean meal and FM which were run at the same time. Results of this work indicate that FMA can replace up to 200 g/kg FM in shrimp diets with supplemental inorganic P. Given the good growth across the range of inclusion without any indication of a growth depression, the low nutrient digestibility of FMA may be due to an atypical response or the product simply does not work with the testing technique.     

O_3胁迫对三种楠木幼苗光合作用的影响及伤害症状

[目的]随着全球O_3浓度升高,O_3胁迫对植物影响的研究已成为研究的热点之一。我国的相关研究较少,主要以农作物为主,且大多局限在北方地区。本研究探讨O_3胁迫下,亚热带三种楠木幼苗光合作用的变化规律及其可见伤害症状,以期为后续研究提供理论依据。[方法]以一年生亚热带乡土树种桢楠、闽楠和刨花楠幼苗为材料,采用开顶式气室(OTCs),研究低浓度O_3、环境大气、100 n L·L-1O_3、150 n L·L-1O_3处理对光合作用的影响及伤害症状。[结果]研究表明:(1)O_3胁迫下,三种楠木幼苗的光合作用受到一定的抑制,气孔导度和净光合速率的相关性降低,对光合有效辐射的利用范围整体减小,出现了明显的光抑制现象。O_3浓度越高,对桢楠和刨花楠光合作用的抑制效果越明显,而100 n L·L-1O_3处理对闽楠光合作用的抑制效果较150 n L·L-1O_3处理明显。环境大气中的O_3浓度均值较低,但由于其较高的O_3浓度峰值,仍对三种楠木幼苗的光合作用产生了一定的抑制。与环境大气相比,低浓度O_3处理通过消除较高的O_3浓度峰值从而缓解了O_3对光合作用的不利影响。(2)O_3胁迫下,桢楠叶片出现褪绿、黄斑和坏死斑的症状,闽楠叶片出现褪绿、黄斑和水渍的症状,刨花楠叶片出现红褐色斑、水渍、坏死斑、卷曲皱缩、失水萎蔫的症状。三种楠木幼苗叶片的伤害症状随O_3浓度的增加而更明显。[结论]三种楠木幼苗叶片的光合作用均受到O_3的抑制,并出现了伤害症状,可作为O_3污染的指示树种。光合作用对O_3敏感性的关系为:刨花楠闽楠桢楠,其中刨花楠对O_3更敏感,伤害症状也较多样,因此指示O_3污染的效果更好。     

基于供水限额的喀什噶尔河流域农业用水模式研究

喀什噶尔河流域是水资源严重短缺的流域。随着流域内工业、农业的发展和人民生活水平的提高,水资源供需矛盾越来越突出。针对喀什噶尔河流域农业用地表水及地下水总量限额,探讨了合理滴灌用水模式的选择;为提高农业地下水的节水能力,实现水资源的合理配置,防止农业需水需求的不断上升提供了有益的建议和规划方案。     

Modelling of planted legume fallows in Western Kenya. (II) Productivity and sustainability of simulated management strategies

Improved fallow is a technology that can help to raise agricultural productivity in systems of poor soil fertility and low financial capital. Models, once calibrated, can be used to investigate a range of improved fallow systems relatively quickly and at relatively low cost, helping to direct experimental research towards promising areas of interest. Six fallow crop rotations were simulated using the WaNuLCAS model in a bimodal rainfall setting in Kenya over a 10 year period: (A) alternating fallow and crop seasons, (B) one season fallow followed by three seasons crop, (C) one season fallow followed by four seasons crop, (D–F) 1–3 seasons fallow periods followed by 3–5 seasons crop. The strategies were tested using a number of fallow growth rates, soil clay contents, and rainfall amounts to determine the interaction of fallow rotation and biophysical variables on maize (Zea mays (L.)) yield and sustainability (organic matter, N₂ fixation, leaching). The best simulated fallow strategies doubled maize yield compared to continuous maize over a 10 year period. Across all biophysical treatments strategy A and B of no more than three consecutive cropping seasons and of one consecutive fallow season yielded the most maize. This was because fallow benefits were largely due to the immediate fallow soil fertility benefit (IFB) rather than the cumulative benefit (CFB). The difference in yield between the two strategies was through a balance between (1) their interaction with the biophysical variables affecting accumulation of organic matter, hence increasing soil fertility and (2) the extra intrinsic soil fertility used for maize productivity by the inclusion of more cropping seasons within the rotation. We propose the following conceptual framework to manage fallows for maximum maize yield: when environmental factors are strongly limiting to fallow and crop growth then fallow strategy A would be the best strategy to employ (less risk but more labour) and when factors are less limiting then strategy B would be the best to employ.     

Ecosystem partitioning of N-glycine after long-term climate and nutrient manipulations, plant clipping and addition of labile carbon in a subarctic heath tundra

Low temperatures and high soil moisture restrict cycling of organic matter in arctic soils, but also substrate quality, i.e. labile carbon (C) availability, exerts control on microbial activity. Plant exudation of labile C may facilitate microbial growth and enhance microbial immobilization of nitrogen (N). Here, we studied ¹⁵N label incorporation into microbes, plants and soil N pools after both long-term (12 years) climate manipulation and nutrient addition, plant clipping and a pulse-addition of labile C to the soil, in order to gain information on interactions among soil N and C pools, microorganisms and plants. There were few effects of long-term warming and fertilization on soil and plant pools. However, fertilization increased soil and plant N pools and increased pool dilution of the added ¹⁵N label. In all treatments, microbes immobilized a major part of the added ¹⁵N shortly after label addition. However, plants exerted control on the soil inorganic N concentrations and recovery of total dissolved ¹⁵N (TD¹⁵N), and likewise the microbes reduced these soil pools, but only when fed with labile C. Soil microbes in clipped plots were primarily C limited, and the findings of reduced N availability, both in the presence of plants and with the combined treatment of plant clipping and addition of sugar, suggest that the plant control of soil N pools was not solely due to plant uptake of soil N, but also partially caused by plants feeding labile C to the soil microbes, which enhanced their immobilization power. Hence, the cycling of N in subarctic heath tundra is strongly influenced by alternating release and immobilization by microorganisms, which on the other hand seems to be less affected by long-term warming than by addition or removal of sources of labile C.     

一起塔式起重机吊重坠落事故的分析

通过对一起塔式起重机吊重坠落事故的分析,找出了电控部分的设计缺陷,并提出了解决对策。     

Hydraulic resistance partitioning between shoot and root system and plant water status of Haloxyolon ammodendron growing at sites of contrasting soil texture

Hydraulic resistance components and water relations were studied on Haloxyolon ammoden-dron,a small xeric tree,growing at sites significantly differed in soil texture.Soil water content,leaf water potential(ψl),xylem water potential(ψx),root water potential(ψroot),leaf transpiration rate(TR) and stomatal conductance(gs) were measured at the two sites during the growing season of 2005 and 2006.Leaf spe-cific hydraulic resistance(Rplant) during the whole growing season,hydraulic resistance of plants(Rp),shoots(Rshoot) and roots(Rroot) in the August of both years were calculated and expressed on leaf area basis.The results showed the proportion of the hydraulic resistance of the aerial part(Rshoot) to the Rp was the same to the proportion of the hydraulic resistance of the soil part(Rroot) to the Rp,indicating that both parts were equivalent important to plant water hydraulic system from soil to leaf.Positive significant corre-lations were found between Rp and Rroot,suggesting that root hydraulics resistance was a major determinant of plant hydraulic resistance(Rp) and transpiration rate.The integrated effect of stomatal control,hy-draulic regulation and morphology adjustment enabled plants at heavy soil site surviving the extreme water deficit period.     

浅议学术期刊常用评价指标及其局限性

家评审供参考综述了学术期刊常用评价指标（影响因子、基金论文比例、总被引频次、立即引用指数、他引率、同行专摘转率、期刊被权威检索工具收录情况）及其在应用中的局限性,为不同类型期刊选取正确的评价指标提