Purpose:Pseudomonas syringae pv. actinidiae causes bacterial canker of kiwifruit and is responsible for severe economic losses and emergence of drug-resistant bacteria. Bacteriophages are viruses that infect target bacterial hosts and may be the best strategy to prevent and control kiwifruit canker disease. The objective of this experiment was to monitor the prevalence of Pseudomonas syringae pv. actinidiae and provide insight for the use of phages in biological control.
Materials and methods: In this study, 52 strains of Pseudomonas syringae pv. actinidiae were isolated from 68 stem samples of kiwi plant (cv. Hongyang & Jinkui). Following polymerase chain reaction (PCR) analysis, 15 isolates belonging to biovar 3 were identified, one of which was named XWY0007 and used as the target strain to isolate the phages. Thirty-six phages were isolated and purified from a total of 51 surface water samples collected in Shanghai. All phages were identified by transmission electron microscopy (TEM) and their host ranges were evaluated. Three phages, designated φXWY0013, φXWY0014 and φXWY0026 were selected and further characterised using one-step growth curve and stability at different temperatures and pH.
Results and conclusions: The isolated phages are promising for use as antimicrobials against bacterial canker in kiwi. This report is regarding Pseudomonas syringae pv. actinidiae and its phages from major areas of kiwifruit cultivation. 相似文献
Despite the fact that soil erosion by water causes considerable on-site and off-site problems, farmers in Europe are reluctant to adopt prevention and control measures when such measures require additional labour and material inputs. This paper documents the impact of multiple sowing of small grains on concentrated flow erosion rates and grain production for a winter triticale field (X Triticosecale Wittmack ex. A. Camus) in the Belgian loess belt. Multiple sowing refers to drilling more than once in zones of concentrated flow erosion in order to increase the total root mass in this zone. Multiple sowing strongly altered the morphology of erosion channels and reduced soil loss significantly. Statistical analysis confirmed that differences in channel dimensions could be explained by the seedling density. Doubling the root mass in the topsoil by multiple sowing, resulted on average in a reduction of soil loss by 42% for the whole growing season. For the winter period, soil loss reduction, mainly attributed to the triticale roots, amounted even to 53%, showing the tremendous impact of seedling roots on soil erosion by concentrated overland flow in the early stages of vegetation growth. Furthermore, total grain yield in the multiple drilled zones was not significantly smaller compared to the conventionally drilled parts of the field. Grain size of cereals was slightly smaller. The results of this case study indicate that double sowing in concentrated overland flow zones may be a viable soil erosion control technique. 相似文献