保卫细胞液泡内pH调节的可逆解聚聚合颗粒物性质初探

为研究保卫细胞液泡(GCV)的pH在气孔保卫细胞渗透调节中的作用及蚕豆(Vicia faba)叶片气孔GCV中的颗粒物的性质,本实验采用激光共聚焦显微术配合pH荧光探针BCECF AM的荧光比值法对ABA诱导的气孔关闭过程进行观测,发现:蚕豆气孔关闭过程中GCV的pH升高约0.5(pH5.3→5.8)。分别用pH5.3和5.8的Mes/Tris缓冲液调控离体开放态GCV的pH,透射电镜(4×104倍)观察负染片说明:pH 5.3对开放态GCV液所含颗粒物的线度及分布密度无实质影响;而pH 5.8的处理使取自开放态GCV液所含颗粒物的线度明显增大、分布密度显著减小;非特异性蛋白酶Proteinase K处理可使GCV中的颗粒物消失。基于GCV内颗粒物的线度、形状及对pH调控和对Proteinase K处理的响应,推测此种颗粒物是蛋白质。     

韩国新村运动及对我国新农村建设的启迪

在详细介绍韩国新村运动内容、发展过程的基础上,对其实施效果进行了评析,并结合我国农村发展现状与经济发展实际,提出了其对我国社会主义新农村建设的启示。     

内蒙古河套灌区秋浇时间对水盐运移和农业环境的影响

土壤盐碱化是当前河套灌区的主要问题,解决这个问题的途径主要是合理灌溉,保证排水,加强管理。河套灌区特有的气候和农业生产条件,决定了灌区秋浇的两大目标,即为翌年春小麦播种和幼苗期生长贮墒及淋洗耕层盐分。本文根据实测资料,从机理上讨论了不同秋浇时间对河套灌区水盐运移和农业环境的影响,得出了早秋浇(白露前后)是河套灌区有效防治盐碱化、改善农业环境、节水节能、增产增收的好措施。     

HA复合肥的养分释放运移特点与土壤培肥效应研究

采用盆栽试验与室内土柱模拟试验方法，研究了腐植酸复合肥中的养分在土体中的释放运移特点以及对土壤的培肥效应，盆栽试验结果表明，与等养分无机肥相比，施用腐植酸复合肥不仅可提高辣椒产量，而且辣椒收获后土壤中的养分含量均有所提高，尤以对土壤中速0效磷含量的影响最为显著；土柱模拟试验结果表明，自制腐植酸复合肥处理各土层中的氮素含量均低于等养分无机肥以及山东产腐植酸复混肥处理。在相同时间内，氮素释放总量比二者分别降低58.34%和48.78%，0-10cm土层中的磷素含量以山东产腐植酸复混肥处理为最高，而在10-30cm土层中则以自制腐植酸复合肥处理最高，30-60cm土层中，各处理之间差异不显著。     

农村人力资本非农化配置动因剖析与应对之策

运用规范研究的方法，从农村人力资本非农化配置现状分析入手，探讨农村人力资本非农化配置引起的不利于推进产业化和现代化、不利于农业产业结构调整和升级、不利于建设社会主义新农村等问题，进而从经济因素（留守农业、农村机会成本大；迁移收益与迁移成本比较）和非经济因素（传统就业心理；分享现代城市文明；追求更高的价值实现）来分析实现非农化配置的动力机制，并提出相关建议：建立农村人才评价体系和激励机制；改善农村就业环境；建立和完善农村社会保障体系，引导农村人力资本合理配置。     

贴体网格的生成与平面二维泥沙运动方程在贴体坐标区域中的变换

针对用平面二维泥沙模型求解不规则区域泥沙运动规律时常遇到的边界复杂问题,引入了贴体坐标的基本原理,推导并离散了贴体网格的生成方程,将泥沙运动方程及边界条件从物理区域向计算区域进行了一一对应转换,最后给出了平面二维泥沙运动方程在贴体坐标下的变换形式。     

大麦黄矮病毒外壳蛋白基因和运动蛋白基因酵母表达载体的构建

根据已知的大麦黄矮病毒GPV株系的外壳蛋白（Coat Protein CP）和移动蛋白（Movement Protein MP）基因序列合成了CP、MP基因的上下游引物。通过PCR扩增获得含有目的基因的片段，经过SalⅠ和PstⅠ双酶切、连接、转化、重组质粒的酶切鉴定及基因测序，构建了酵母表达载体pGBKT7-GPV-CP和pGBKT7-GPV-MP，用于在酵母双杂交分析中表达诱饵融合蛋白，为进一步筛选小麦cDNA文库内与大麦黄矮病毒互作的寄主因子、克隆寄主因子、推测其种类和功能奠定了基础。     

滴灌土壤水分运动研究进展

滴灌土壤水分运动的研究对于滴灌系统的合理设计有着重要的理论和现实意义。在国内外研究的基础上,概括了在滴灌条件下土壤水分运动规律及其影响因素、湿润体浸润形状、湿润锋的运移规律、点源入渗的几个经典模型及各自的优缺点等方面的研究现状。在此基础上,进一步探讨了滴灌土壤水分运动研究中还存在的问题,如滴灌点源交叉区域内的土壤水分变...     

Consequences of adult and juvenile movement for marine protected areas

Adult and juvenile mobility has a considerable influence on the functioning of marine protected areas. It is recognized that adult and juvenile movement reduces the core benefits of protected areas, namely protecting the full age–structure of marine populations, while at the same time perhaps improving fisheries yield over the no-reserve situation through export of individuals from protected areas. Nevertheless, the study of the consequences of movement on protected area functioning is unbalanced. Significant attention has been paid to the influence of certain movement patterns, such as diffusive movement and home ranges, while the impacts of others, such as density-dependent movements and ontogenetic migrations, have been relatively ignored. Here we review the diversity of density-independent and density-dependent movement patterns, as well as what is currently known about their consequences for the conservation and fisheries effects of marine protected areas. We highlight a number of ‘partially addressed’ issues in marine protected area research, such as the effects of reserves targeting specific life phases, and a number of essentially unstudied issues, such as density-dependent movements, nomadism, ontogenetic migrations, behavioral polymorphism and ‘dynamic’ reserves that adjust location as a realtime response to habitat changes. Assessing these issues will be essential to creating effective marine protected area networks for mobile species and accurately assessing reserve impacts on these species.     

The influence of water stress and air velocity on growth of Impatiens walleriana and Petunia × hybrid

Impatiens walleriana ‘DeZire’ and Petunia × hybrid ‘Tidal Wave’ were subjected to a combination of water stress and exposure to wind to evaluate the potential application of these treatments as an alternative to chemical growth retardation. Air velocities evaluated were 0.1, 0.2, 0.4, 0.8, 1.5, and 4.5 m s⁻¹ and irrigation treatments were either control or 45% water stress based on irrigation occurring when the weight of the plant (plant, pot and peat) was reduced by 25% (control) or 45% (water stress) of the initial weight. The experiment was repeated twice; once in spring and once in summer.