Recent advances in molecular genetics of forest trees

The use of molecular markers has greatly enhanced our understanding of the genome structure of forest trees. Conifers, in particular, have a relatively large genome, containing a very high proportion of repeated DNA, consisting of tandemly repetitive and dispersed repetitive DNA sequences. The nature of highly conserved tandemly repetitive rRNA genes has been investigated in a number of tree species, and their sites mapped on specific chromosomes by fluorescent in situ hybridization (FISH). Different families of retrotransposons (IFG, and TPE1) have been isolated and characterized from the dispersed repetitive DNA of pines. Genome maps have been constructed in a number of forest tree genera: Pinus, Picea, Pseudotsuga, Cryptomeria, Taxus, Populus, and Eucalyptus. EST databases have been established from cDNA clones of pines and poplars. The structure and maternal or paternal modes of inheritance of organelle genomes have been investigated in forest trees. Comparative mapping in conifers has shown that gene families are conserved across genera. Due to lack of polyploidy in conifers, the evolution of this group of trees may have occurred primarily by duplication and dispersal of genes, probably by retrotranspositions, to form complex gene families. The evolution of angiosperm tree species has presumably involved both gene duplication as well as genome duplication (polyploidy). Application of genetic engineering has shown that genes from phylogenetically unrelated organisms can be introduced and expressed in trees, thus offering prospects of genetic improvement of forest trees. This revised version was published online in August 2006 with corrections to the Cover Date.     

安徽省耕地资源系统分析与保护对策研究

本文对安徽省耕地资源进行了系统诊断,对影响耕地数量减少因素进行灰色关联度分析,对人均耕地占有量运用GM（1,1）模型进行预测。在此基础上,提出耕地保护对策及措施。     

温度对棉纤维强度及超分子结构的影响

利用短季棉中棉所16号品种, 研究了温度对棉纤维断裂比强度与超分子结构的影响, 结果 表明： 纤维断裂比强度随铃龄增大而升高; 相同铃龄随温度下降断裂比强度降低。 较高 温度条件下α角随铃龄增大不断宽化(变大), 对纤维强度提高不利; 较低温度条件下α角 随纤维发育不断变小(优化), 某种程度上有利于纤维强度提高;     

Effects of plastic film mulch and tillage on maize productivity and soil parameters

This paper determined the effects of mulching time for double furrows and ridges using plastic film on soil water status, grain yield of maize, soil quality, and economic benefits. The study was conducted in a typical semiarid area during two growing seasons of 2006–2007 with the following three treatments: (i) plastic film mulching at maize sowing with conventional tillage, and the film was removed at harvest (CK); (ii) mulching applied 30 d before sowing with conventional tillage, and the film was removed at harvest (T1); and (iii) mulching at sowing with no-tillage, and the film left on the field after harvest in the first season and used for mulching in the second season (T2). The T1 in both years and T2 in the second year (2007) improved soil water content (in the 0–60 cm layer) and temperature (10 cm) at sowing compared with CK. After the two seasons, the soil water content was significantly higher in the 0–80 cm soil layer in CK and T1, and in the 0–120 cm soil layer in T2; however, it decreased significantly in 140–200 cm soil layer in CK and T1, compared to their initial values at sowing in April 2006, and there was no significant change in T2. The rainfall storage in the 0–200 cm soil layer during the non-growing season (late September 2006 to late April 2007) was 18.2 mm in CK, 34.0 mm in T1, and 59.7 mm in T2, and the rainfall storage in 100–200 cm soil layer was 16.5 and 18.6 mm higher in T2 than in CK and T1, respectively. In 2006, there were no significant differences in yield and water use efficiency (WUE) in all treatments. In 2007, the yield in T1 was significantly higher than in T2, but yields in T2 and CK were not significantly different, and there was no significant difference in WUE among treatments. Soil organic carbon (SOC) (0–20 cm) decreased in CK and T1, but increased (by 2.7%) in T2 at harvesting in September 2007 from the initial value of sowing in April 2006. The ratio of output to input was 1.32:1 for CK, 1.40:1 for T1, and 1.67:1 for T2 averaged across the two seasons. Therefore, T2 was a more sustainable model for increasing water storage, producing greater economic benefit and maintaining SOC balance for maize production in semiarid area.     

高寒阴湿地区半开放型暖棚牛舍冬季内环境监测评价

在高寒阴湿的寒冷地区修建半开放型暖棚牛舍,充分利用塑料膜的温室效应,能够达到保暖增膘的饲养目的,本课题研究采用定期定点观测的方法,在冬春季扣膜后,对暖棚牛舍和普通圈舍的采光系数、入射角、透光角、舍内外的温度、湿度及CO2、NH3、H2S等主要技术指标进行了监测和对比.结果表明暖棚牛舍(试验组)舍内11月份～3月份平均温度为8.78℃;平均湿度为55.53%;舍内的CO2含量平均为0.141%;NH3平均为19.02 mg/m3;H2S平均为9.91 mg/m3;采光系数为0.86;入射角为64°;透光角为45°.普通牛舍(对照组)的平均温度为0.25℃;平均湿度为66.75%;舍内的CO2平均为0.137%;NH3平均为18.49 mg/m3;H2S平均为9.64 mg/m3;采光系数为0.082;入射角为35°;透光角为10°.试验组牛的授配率、产仔成活率、繁活率分别比对照组提高了24%、17%、50%,而患病率降低了2.6%.     

基于GOES数据和弱约束变分的地表水热通量估算

基于弱约束的四维变分方法和陆面过程模式发展了一个地表温度的陆面数据同化系统。本文反演了地球静止业务环境卫星(Geostationary operational environmental satellite,GOES)的地表温度,并将反演的地表温度同化入陆面过程模式,改进陆面过程模式中地表水热通量的估算精度。以弱约束的变分方法通过在代价函数中增加弱约束项代替陆面过程模式动力方程组中存在的模式误差,构建新的代价函数并对其优化,从而改善模式中显热与潜热的估算精度。将GOES地表温度与实测地表温度进行比较,其均方根误差(RMSE)作为试验中的观测误差。选择美国通量网AmeriFlux中2个主要农业站点的气象和通量数据作为试验数据,对同化系统进行驱动和验证。结果表明同化后的地表温度、潜/显热估算精度均有提高。其中,各站地表温度RMSE平均仅为1 K,显热通量平均RMSE下降22 W/m2,潜热通量平均RMSE下降26 W/m2。因此结合陆面过程模式的弱约束变分方法同化GOES反演温度产品估算近地表水热通量的方法是有效且可行的。     

保水剂和灌水对小粒咖啡苗木的节水调控效应

为探讨热带特色作物小粒咖啡的高效节水模式,研究3个保水剂水平(高保、低保和无保)和3个灌水水平(高水、中水和低水)对小粒咖啡苗木生理、生长、干物质及水分利用的影响。结果表明:与无保相比,低保分别提高叶绿素、类胡萝卜素和根系活力11.8%、13.4%和52.2%,而分别降低可溶性糖、丙二醛和脯氨酸24.9%、24.3%和55.8%,同时提高总干物质和水分利用效率31.0%和35.9%;而高保分别降低叶绿素、类胡萝卜素、丙二醛和根系活力3.1%、2.4%、13.5%和6.3%,提高叶片可溶性糖和脯氨酸3.7%和75.1%,降低总干物质21.3%,提高水分利用效率8.6%。与低水相比,中水分别提高总干物质、耗水量和水分利用效率89.8%、44.5%和33.2%,高水分别提高总干物质、耗水量和水分利用效率172.8%、104.8%和34.0%。和无保低水相比,低保中水的水分利用效率增幅最大为112.7%,同时提高总干物质158.9%,分别提高叶片相对含水率、叶绿素、类胡萝卜素和根系活力24.4%、19.5%、25.8%和149.9%,分别降低可溶性糖、丙二醛和脯氨酸38.3%、36.4%和68.7%。从高效节水的角度考虑,低保中水为最适宜的搭配方式。     

双活塞液粘调速离合器动态特性研究

为了解决液粘调速离合器摩擦副偏磨问题,提高液粘调速离合器摩擦片运动系统的响应速度,提出了一种新型双活塞结构液粘调速离合器。基于摩擦片受力模型,推导出了双活塞结构液粘调速离合器摩擦片间油膜的动态平衡方程及传递函数,并对其动态特性进行了理论分析及仿真研究。结果表明:双活塞结构液粘调速离合器与单活塞的相比输出响应速度明显提高;在输出转速为80 rad/s时,其响应时间可由52 s减少到22 s。     

黑土区调亏灌溉条件下大豆耗水规律试验研究

针对黑土区存在的降雨量时空分布不均,水资源短缺,作物水分利用效率较低的实际问题。2013年在黑龙江省北安农垦分局红星农场进行了盆栽试验,研究了调亏灌溉条件下的大豆耗水规律。结果表明大豆各生育阶段耗水量按结荚-鼓粒、开花-结荚、鼓粒-成熟、分枝-开花、出苗-分枝、播种-出苗阶段依次递减;产量、水分利用效率与耗水量之间均呈二次抛物线关系。耗水量为62.5kg/盆时,水分利用效率先出现最大值,耗水量达84.76kg/盆时,产量(52.9g/盆)最高,最高值出现时间较水分利用效率偏后。在试验的基础上,建立了基于Jensen模型的黑土区大豆水分生产函数。研究结果可为黑土区大豆节水灌溉管理提供一定的理论依据。     

中耕机械的结构设计研究

中耕是农作物田间管理的重要作业项目,中耕机械是指在农作物生长过程中进行松土、除草、培土、施肥等作业的土壤耕作机械。阐述中耕机械的分类方法及总体构造,详细介绍其主要工作部件的结构及作用,为中耕机械的使用提供参考。