1例藏獒三氮脒中毒的生化检验及治疗

用三氮脒以3.5 mg/kg的剂量对患有附红细胞体病的藏獒进行肌肉注射,隔日用同等剂量的三氮脒肌肉注射,24 h后,藏獒发生以神经症状为主的中毒现象,并表现出食欲废绝,后肢发软,四肢抽搐呈划水状,卧地不起,昏迷,体温正常临床症状。经血清生化检验,藏獒的尿素氮、肌酸激酶等的生化指标明显升高,血糖降低占病例的100%。经过5 d的药物治疗后,中毒藏獒基本恢复健康。     

农机检测实验室样品管理研究

样品管理是实验室工作中的重要环节，规范化、制度化的样品管理工作可保障实验室管理体系的有效运行。阐述实验室样品管理的总体要求与原则，从样品接收、标识、确认、流转、贮存、处理等环节人手，介绍样品全过程跟踪管理方法，为做好实验室样品管理工作提供参考。     

Pathological analysis of batch safety testing of veterinary vaccines using small laboratory animals

Batch safety tests (BSTs) of veterinary vaccines are conducted using small laboratory animals to assure the safety of vaccines according to several criteria, including clinical signs and change in body weight. Although the latter is used as an evaluation index in BSTs, there have been no reports on the internal changes that affect body weight during the test period. Therefore, we analyzed BST via pathological examination of the tested animals. Here, BSTs were performed for 176 batches using mice and 126 batches using of guinea pigs. Most of the gross findings could be classified into four lesion types (nodules, adhesions, ascites, no apparent signs), with only one vaccine inducing lesions that could not be classified into any of these four types. Histopathological examination revealed that the reactions caused by BST were pyogenic and/or granulomatous inflammation. Nodular or adhesive lesions comprised more severe pyogenic granulomatous inflammation than ascites or cases with no apparent gross lesions. These nodular or adhesive lesions were more frequently induced by vaccines that contained an adjuvant than by vaccines that did not contain an adjuvant. The cases with “exceptional” gross findings histologically presented severe necrosis of the hematopoietic system. Additional testing showed that these “exceptional” lesions were induced when a specific type of light liquid paraffin was injected along with other vaccine additives. Our results show that body weight loss and/or lesions during BST were induced by proinflammatory properties of the tested vaccines and that BST is a sensitive method for detecting unexpected effects of vaccine components.     

A proposal to leverage high-quality veterinary diagnostic laboratory large data streams for animal health,public health,and One Health

Test data generated by ~60 accredited member laboratories of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) is of exceptional quality. These data are captured by 1 of 13 laboratory information management systems (LIMSs) developed specifically for veterinary diagnostic laboratories (VDLs). Beginning ~2000, the National Animal Health Laboratory Network (NAHLN) developed an electronic messaging system for LIMS to automatically send standardized data streams for 14 select agents to a national repository. This messaging enables the U.S. Department of Agriculture to track and respond to high-consequence animal disease outbreaks such as highly pathogenic avian influenza. Because of the lack of standardized data collection in the LIMSs used at VDLs, there is, to date, no means of summarizing VDL large data streams for multi-state and national animal health studies or for providing near-real-time tracking for hundreds of other important animal diseases in the United States that are detected routinely by VDLs. Further, VDLs are the only state and federal resources that can provide early detection and identification of endemic and emerging zoonotic diseases. Zoonotic diseases are estimated to be responsible for 2.5 billion cases of human illness and 2.7 million deaths worldwide every year. The economic and health impact of the SARS-CoV-2 pandemic is self-evident. We review here the history and progress of data management in VDLs and discuss ways of seizing unexplored opportunities to advance data leveraging to better serve animal health, public health, and One Health.     

Full Aero-elastic Bridge Model Design of Long Span Arch Bridge

Aero-elastic model design is the precondition of full bridge aero-elastic model tunnel tests. The full aero-elastic bridge model design of long span arch bridge is discussed. The effect on the model design of different transverse rod space and bend stiff is analyzed in detail. Different scheme need to be designed according to the transverse rod stiff. Considering the changing section dimensional arches, the method of projecting the spatial arches to the plane arches, then designing the plane arches, at last projecting the plane arches to the spatial arches is proposed at first. The results show that the method is effective.     

地下滴灌灌水器出口正压试验研究

通过试验 ,研究了灌水器类型、流量、工作压力和土壤初始含水量对地下滴灌灌水器出口正压的影响规律。结果表明 :灌水器埋入土壤后 ,其出流由于受土壤等因素的限制 ,在灌水器出口处产生了一定的正压 ,该正压随着灌水历时的延长而增大。影响地埋灌水器出口正压的主要因素是灌水器的额定流量和土壤初始含水量     

铰链密封式传动滚子链的道路试验研究

通过30000km道路行驶试验研究表明，密封滚子链的磨损形式，对销轴和套筒而言是以疲劳磨损为主,伴随有粘着磨损与磨粒磨损；对链板和O形圈而言主要是疲劳磨损，并且O形圈的磨损要比链板的磨损严重得多。销轴和套筒表层磨损后产生了循环硬化特性，有利于提高其铰链副的耐磨性。