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81.
YAN Rui SHAN Hu LIN Lin DIAO Jia-yu ZHANG Ming ZHU Yan-he TAN Wu-hong WEI Jin 《园艺学报》2015,31(6):967-972
AIM: To observe the effect of high glucose on the protein expression of calreticulin (CRT) and its association with cell apoptosis and mitochondrial dysfunction in the cardiomyocytes. METHODS: AC-16 cardiomyocytes were randomly divided into normal glucose group, high glucose group, high glucose+ CRT siRNA group and isotonic control group. The cell apoptotic rate, reactive oxygen species (ROS), mitochondrial membrane potential level, respiratory enzyme activity, and protein expression of CRT were observed. RESULTS: Compared with the cardiomyocytes in normal glucose group, the apoptotic rate and ROS production of cardiomyocytes increased in high glucose group, accompanying with the decreases in the mitochondrial membrane potential level and enzyme activitiy of the respiratory chain. The protein expression of CRT was significantly increased in high glucose group. However, compared with high glucose group, high glucose+ CRT siRNA decreased the expression of CRT and attenuated the damage of mitochondria, but CRT siRNA did not reduce the ROS level in cardiomyocytes. CONCLUSION: High glucose brings about CRT over-expression to induce mitochondrial injury, thus increasing myocardial apoptosis. 相似文献
82.
LI Su-juan JIN Ke-ke ZHAO Ya-ping WANG Peng ZHAO Wen-xi GAO Kai-xuan YANG Wen-juan WANG Jia-lin WU De-ping 《园艺学报》2015,31(7):1259-1265
AIM: To explore the mechanisms of fluctuant high blood glucose-induced apoptosis of hepatocytes. METHODS: SD rats were randomly divided into normal control group (N), stable high blood glucose group (S), fluctuant high blood glucose group (F) and insulin group (I). Diabetic rats were induced by intraperitoneal injection of streptozotocin (65 mg/kg), and the fluctuant high blood glucose animal model was induced by intraperitoneal injection of ordinary insulin and glucose at different time points every day. The blood glucose fluctuation patterns of the animals in F group within 12 weeks were similar every day and no significant difference of the HbA1c concentration was observed compared with S group, indicating that the fluctuant hyperglycemia was successfully established in F group. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the content of malondialdehyde (MDA) and nitric oxide (NO) in the homogenate of the liver tissues were detected by colorimetry. The mRNA and protein levels of JNK, p-JNK, Bax and Bcl-2 were examined by RT-PCR and Western blot. RESULTS: After 12 weeks, the increases in the intakes of food and water, the urine output, and the abnormal liver function were observed in S group, I group and F group. Compared with N group, the MDA level was increased, the content of NO and the activity of SOD and GSH-Px were decreased, and up-regulation of JNK mRNA and p-JNK and Bax proteins, and down-regulation of Bcl-2 were also found in S group, I group and F group. The above effects were more obviously showed in F group. CONCLUSION: Oxidative stress activates JNK-MAPK signaling pathway, which is involved in fluctuant high glucose-induced apoptosis of hepatocytes. 相似文献
83.
Effectiveness of selection for quality traits during the early stage in the potato breeding population 总被引:2,自引:0,他引:2
Potato cultivars resistant to cold‐temperature sweetening are of major importance to the processing industry producing both chips (crisps) and French fries. When most modern potato cultivars are maintained in cold storage to retard sprouting, the tubers accumulate reducing sugars, and the products become an unacceptable brown colour when fried. Selection for better processing quality during the early generations of a breeding programme could be of considerable advantage. Using a portable ‘sugarmeter’, which requires only a drop of sap from the tuber on a test strip, many samples can be efficiently surveyed for low sugar as early as the F1 generation. Using seedlings of three test crosses, glucose and specific gravity of field‐grown tubers, minitubers from greenhouses and microtubers from in vitro culture were compared after cold treatment. Although the mean glucose levels of minitubers and microtubers were higher than field‐grown tubers, the correlation between the glucose contents of the three types of tubers was fairly high. A considerable genetic improvement was noted when progenies were grown as minitubers or microtubers, even though the response to selection for low glucose levels in minitubers and microtubers was lower than from direct selection from field‐grown tubers. The specific gravity of field‐grown tubers showed a significant association with freshly harvested minitubers and microtubers. Selection for low glucose content in minitubers can therefore save considerable resources in a breeding programme. 相似文献
84.
日粮添加木聚糖酶对肉鸡小肠葡萄糖吸收及其转运载体基因表达影响 总被引:7,自引:0,他引:7
156羽1日龄AA肉雏鸡随机分为对照组和试验组,对照组饲以小麦基础日粮,试验组在基础日粮中添加1000mg/kg木聚糖酶,试验期为30d。与对照组相比,试验组4周内平均日增重提高4.81%(P〈0.01),料重比下降5.00%(P〈0.05);肠系膜静脉血清中葡萄糖的含量提高5.56%(P〈0.05)。采用半定量RT-PCR方法测定十二指肠和空肠黏膜SGLT1 (sodium/glucose cotransporter 1)和GLUT2(glucose transporter 2)mRNA的表达,发现木聚糖酶显著增加十二指肠SGLT1 mRNA的表达,增幅为33.30%(P〈0.05),试验组空肠SGLT1 mRNA的表达量比对照组增加8.21%,但差异不显著(P〉0.05)。木聚糖酶对十二指肠和空肠GLUT2 mRNA表达均无显著影响。提示木聚糖酶主要作用在小肠上段,通过上调SGLT1的表达而影响肉鸡小肠葡萄糖吸收。 相似文献
85.
Logistic模型模拟乙醇发酵产物动力学 总被引:1,自引:0,他引:1
乙醇发酵产物动力学的研究有助于更好的认识发酵过程,为其工业放大及生产操作条件的优化提供理论基础。基于Logistic方程的菌体生长动力学模型可较好的描述细胞生长期及细胞自身抑制作用,但由于该模型方程中的比例参数与积分常数没有明显的生物学意义,使其应用受到了限制。该文从生物学与化学工程学结合角度对Logistic模型方程重新参数化,将发酵产物乙醇生成动力学与酵母生长动力学方程类比,给出了乙醇浓度的显式函数模型,模型中不再出现酵母菌浓度变量,大大简化了模型,并且赋予参数其物理意义;在研究了以葡萄糖和玉米淀粉为原料乙醇质量浓度、总糖质量浓度在不同底物质量浓度和料液比条件下随发酵时间的变化规律的基础上运用该模型拟合了以葡萄糖和玉米淀粉为原料进行乙醇发酵的试验数据,结果表明:模型值与试验数据具有较好一致性,拟合度均大于0.97,可见该重新参数化的Logistic模型可以描述发酵生产乙醇过程中产物乙醇的动力学行为,具有预测工业上实际发酵过程中乙醇浓度的潜力。 相似文献
86.
史庭足 《林业机械与木工设备》2011,39(5)
罗茨鼓风机叶轮的传统设计方法是图解法,这种方法误差较大,浪费时间,在加工过程中需要反复进行修改,而且叶轮运转时容易产生噪声,效率低.针对这一问题,根据啮合原理中求解共轭曲线的解析法来求解叶轮的共轭曲线并构建精确的数学模型,以缩短叶轮的设计时间,提高叶轮的设计效率和设计品质. 相似文献
87.
Analysis of Nitrite Accumulation During Denitrification 总被引:1,自引:0,他引:1
To realize the purpose of enhanced nitrogen removal (TN<5 mg·L-1) in step feed process treating urban wastewater, by using SBR reactor, the nitrite accumulation, variation of pH and ORP as well as dynamic properties during denitrification with methanol and glucose as the sole carbon sources are investigated in details. The results show that for both carbon sources, serious nitrite accumulations are observed during denitrification under different ratios of nitrogen and carbon (C/N). With identical C/N, when glucose is used as carbon source there is more maximum NO2--N accumulation concentration. However, NO3--N reduction rate is faster than NO2--N reduction rate for both carbon sources. Furthermore, with the increase of C/N, the maximum NO2--N concentration increases and the time in which the NO2--N achieves the highest concentration is accordingly shortened. For glucose, however, under high C/N ratio (C/N=29.3) both the denitrification rate and NO2--N accumulation concentration decrease. Besides, the changes of pH and ORP reflect the variation of NO2--N accumulation concentration well because pH and ORP have the significant relationship with NO2--N concentration. The second inflexion on curves indicates the real end of denitrification process. 相似文献
88.
单变量动态Gamma分布模型及贝叶斯预测 总被引:2,自引:0,他引:2
陈传勇 《仲恺农业技术学院学报》2004,17(1):49-52
给出了观测值服从单变量Gamma分布,并在自然参数与状态参数之间满足线性关系ωt=F'tθt的假设下,利用共轭分布给出了相应模型的修正递推及其预测公式. 相似文献
89.
应用自动微分技术的CF—PCG方法及其效率分析 总被引:1,自引:0,他引:1
张海斌 《中国农业大学学报》2001,6(2):24-28
CF-PCG算法是牛顿法和预优共轭梯度法结合起来求解牛顿方程的一种非精确牛顿法。笔者将自动微分技术应用到该算法中,并证明应用自动微分技术的CF-PCG方法具有更高的效率。 相似文献
90.
本文成功地研制了PVC葡萄糖氧化酶(GOD)柱,用流动装置和I电极探讨了磁场对GOD活性的影响:密闭条件下磁化,GOD活性有所降低,敞开条件下则活性有所增强,并对其结果进行了初步探讨。 相似文献