农村居民食品安全意识的实证研究———基于东川区农村的调查分析

本文以对集义镇９５位居民和阿旺镇９０位居民进行的问卷调查为基础,通过构建 Ｌｏｇｉｔ模型,运用 ｅｖｉｅｗｓ６０软件对模型进行实证分析,研究了农村居民对食品安全认知的现状及影响因素。研究发现,虽然农村居民对食品安全有一定的认识,但对食品标志及具体区别的认知明显缺乏;男性中 ２０～４０岁、学历高的农村居民对三种安全认证食品的认知程度较高。计量分析表明,农村居民的性别、年龄和学历显著影响食品安全认知。     

把握《纲要》特点 确保食物安全

国务院办公厅新发布的《中国食物与营养发展纲要(2014—2020年)》,是一个具有民本性、科学性、宏观性、创新性和食安性的纲领性文件。在新形势下,要全面把握新《纲要》的"五性"特点,确保中国食物安全,推动食物与营养健康发展。     

Comparison of three commercial rapid agglutination test kits for identification of coagulase positive staphylococci from foods and animals.

Three rapid agglutination assays for the identification of Staphylococcus aureus Monostaph (Bionor A/S, Skien, Norway), Staphyslide-Test (BioMerieux, Lyon, France) and Staph-Rapid-Test (Roche, Basel, Switzerland), were compared. A total of 104 Gram-positive, catalase positive cocci were tested: Nineteen Staphylococcus reference strains comprising 15 spp. (4 strains were coagulase positive), and 7 Micrococcus reference strains comprising 4 spp.; 22 food isolates comprising 13 S. aureus, 8 coagulase positive Staphylococcus spp., and 1 Micrococcus sp.; 56 animal isolates comprising 11 S. aureus, 9 S. hyicus subsp. hyicus, 2 S. intermedius, 15 coagulase positive and 19 coagulase negative Staphylococcus spp. Totally 54 strains were coagulase positive. Considering agglutination of a coagulase positive strain as a correct identification, Monostaph, Staph-Rapid-Test, and Staphyslide-Test correctly identified 52 (96.3%), 47 (87.0%) and 48 (89.0%) of the coagulase positive staphylococci, respectively. Monostaph, Staph-Rapid-Test and Staphyslide-Test showed 1 (2.0%), 4 (8.0%) and 4 (8.0%) false positive reactions respectively. Monostaph, Staph-Rapid-Test and Staphyslide-Test gave 0 (0.0%), 6 (5.8%) and 7 (6.7%) non-interpretable reactions, respectively. Monostaph may be a good alternative to the tube-coagulase test for rapid and reliable identification of coagulase positive staphylococci from both food and veterinary sources. However, false negative reactions may occur with coagulase positive strains of S. hyicus subsp. hyicus and S. intermedius.     

我国区域间气候智能型农业平衡发展及障碍度研究

当前全球气候变化的严峻挑战下，实施气候智能型农业（Climate-Smart Agriculture，简称CSA）的理念与方法有助于保障我国农业生产的稳定性与可持续性。本研究以中国九大农业区为研究对象，选取2010—2021年省级面板数据，运用熵权法、平衡发展指数模型、障碍度模型，对中国各农业区的气候智能型农业发展指数（CSA-DI）、气候智能型农业平衡发展指数（CSA-BDI）、气候智能型农业发展障碍因素进行了实证研究。结果显示：2010—2021年，大部分农业区的CSA-DI和CSA-BDI均呈现提升趋势，其中四川盆地及周边地区、东北平原区、黄淮海平原区、云贵高原区表现尤为显著。尽管重点产区在系统性、整体性与协同性原则方面取得了初步成效，但在粮食安全、气候变化适应性与减缓农业碳排放等方面仍存在提升的空间。部分农业区则面临对资源禀赋过于依赖、经济发展与农业发展难以协同等问题。根据评价结果本研究提出了一系列政策建议，包括匹配模块化发展方案、划定资源保护红线、进行CSA技术归纳与应用、推动“农林牧融合”综合试点等。这些建议旨在促进各地区气候智能型农业的发展，实现成果共享与责任共担。     

Meat Juice Serology and Improved Food Chain Information as Control Tools for Pork‐Related Public Health Hazards

The seroprevalence of Salmonella spp., pathogenic Yersinia spp., Toxoplasma gondii and Trichinella spp. was studied in 1353 finishing pigs from 259 farms that were allocated according to farm types: large fattening farms (≥1000 pig places), small fattening farms (< 1000 pig places) and farrow‐to‐finish farms. The antibodies were analysed with commercial ELISA kits in meat juice samples that were collected at Finnish slaughterhouses. Salmonella antibodies were rare (3% of pigs, 14% of farms) when the cut‐off optical density (OD) value 0.2 was used. Antibodies to pathogenic Yersinia spp. and T. gondii were detected in 57% of pigs and 85% of farms (OD ≥0.3) and in 3% of pigs and 9% of farms (OD ≥0.15), respectively. No antibodies to Trichinella spp. were detected (OD ≥0.3). The European Food Safety Authority (EFSA) considers Salmonella spp., Yersinia enterocolitica, T. gondii and Trichinella spp. as the most relevant biological hazards in the context of meat inspection of pigs. The seroprevalence of these important zoonotic pathogens was low in Finland, except that of Yersinia. The seroprevalence of Toxoplasma was significantly higher in pigs originating from small‐scale fattening farms (P < 0.05). Strong positive correlation was observed at the animal level between Salmonella and Yersinia seropositivity and between Salmonella and Toxoplasma seropositivity (P < 0.05). We suggest that these results reflect the level and importance of biosecurity measures applied on the farms. Meat juice serology at slaughter is a useful tool for targeting measures to control these pathogens. The information obtained from analyses should be used as part of the food chain information (FCI).     

Effects of utilization of local food by‐products as total mixed ration silage materials on fermentation quality and intake,digestibility, rumen condition and nitrogen availability in sheep

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate in vivo digestibility of total mixed ration (TMR) silage with food by‐products for dairy cows, and the ruminal condition and nitrogen (N) balance were examined. Five by‐products (i.e. potato waste, noodle waste, soybean curd residue, soy sauce cake and green tea waste) were obtained. Four types of TMR silage were used: control (C) containing roughage and commercial concentrate, T1:20% and T1:40% containing the five by‐products replacing 20% and 40% of the commercial concentrate on a dry matter (DM) basis, respectively, and T2:40% containing three by‐products (potato waste, noodle waste and soybean curd residue) replacing 40% of the commercial concentrate on a DM basis. The ingredients were mixed and preserved in oil drum silos for 4 months. The TMR silages showed 4.02–4.44% and 1.75–2.19% for pH and lactic acid contents, respectively. The digestibility of DM and neutral detergent fiber, and total digestible nutrient content were higher (P < 0.05) for T2:40% feeding than for C feeding. Urinary nitrogen excretion tended to be lower (P = 0.07) for T2:40% than for C. The results suggested 40% replacing of commercial concentrate by using the three food by‐products can be most suitable for TMR silage.     

Calliandra calothyrsus as a Supplement for Milk Production in the Kenya Highlands

Two on-farm experiments and one on-station observation were conducted between July 1994 and September 1995 to study the effect of supplementation with fresh fodder of Calliandra calothyrsus on milk production from grade Friesian and Ayrshire cows in the second trimester of their lactations. The cattle were kept under zero-grazing systems on small farms in the coffee-based land use system at altitudes of 1500 to 1800 m on the slopes of Mt Kenya. These cows form a pivotal part of the farming system since they produce both milk for sale and manure for crop production. Milk production is normally in the region of 10 kg/cow per day when the animals are fed on a diet based on Napier grass and crop residues, together with 2–4 kg/day of commercial concentrate. In terms of milk production, 3 kg of fresh calliandra had the same effect on yield as 1 kg of additional dairy meal and, at normal production levels, the effects of the two supplements were strictly additive. Calliandra had a marked positive effect (about a 10% increase) on the butterfat content of the milk, a factor that was highly valued by farmers, even though institutional buyers as yet offer no premium price for milk quality. The average small farm can produce enough calliandra fodder to supplement two dairy cows and some additional small stock from relatively underutilized niches along the farm perimeter and terrace risers, without any adverse effect on current levels of crop production.     

动物源食品中酰胺醇类药物及其代谢物残留检测超高效液相色谱-串联质谱法研究

建立了动物源食品中酰胺醇类药物(氯霉素CAP、甲砜霉素TAP、氟苯尼考FF)及其代谢物(氟苯尼考胺FFA)残留检测的超高效液相色谱-串联质谱(UPLC-MS/MS)法。样品经氨化乙酸乙酯提取,正已烷脱脂,氨化乙酸乙酯反萃取,电喷雾正/负离子多反应监测(MRM)模式检测。四种药物在0.2~50μg/L的系列浓度范围呈线性相关,样品中CAP的检测限为0.1μg/kg,定量限为0.2μg/kg;TAP、FF、FFA检测限为0.5μg/kg,定量限为1.0μg/kg。在0.2~5μg/kg的添加浓度范围内平均回收均为80%~120%,变异系数均小于15%。结果表明该方法简单快速、灵敏度高、重复性好,适用于动物源食品中酰胺醇类及其代谢物残留检测。     

Evaluation of Pet‐Related Management Factors and the Risk of Salmonella spp. Carriage in Pet Dogs from Volunteer Households in Ontario (2005–2006)

The purpose of this study was to determine pet‐related management factors that may be associated with the presence of Salmonella spp. in feces of pet dogs from volunteer households. From October 2005 until May 2006, 138 dogs from 84 households in Ontario were recruited to participate in a cross‐sectional study. Five consecutive daily fecal samples were collected from each dog and enrichment culture for Salmonella spp. was performed. A higher than expected number of the dogs (23.2%; 32/138) had at least one fecal sample positive for Salmonella, and 25% (21/84) of the households had at least one dog shedding Salmonella. Twelve serotypes of Salmonella enterica subsp. enterica were identified, with the predominant serotypes being Typhimurium (33.3%; 13/39), Kentucky (15.4%; 6/39), Brandenburg (15.4%; 6/39) and Heidelberg (12.8%; 5/39). Univariable logistic regression models were created with a random effect for household to account for clustering. Statistically significant risk factors for a dog testing positive included having contact with livestock, receiving a probiotic in the previous 30 days, feeding a commercial or homemade raw food diet, feeding raw meat and eggs, feeding a homemade cooked diet, and having more than one dog in the household. In two‐variable models that controlled for feeding raw food, the non‐dietary variables were no longer statistically significant. These results highlight the potential public health risk of including raw animal products in canine diets.