元江干热河谷地区的尾叶桉扦插育苗试验

从广东湛江引进尾叶桉组培苗 ,先种植建立采穗圃 ,然后采其嫩枝作为插条。用不同浓度的 5种生长激素处理插条、并在 3种基质上作尾叶桉的扦插育苗试验 ,经扦插生根天数和生根率的对比 ,结果表明 :不同基质间的插条生根天数存在极显著差异 ;不同生长激素浓度和育苗基质间插条的生根率也存在极显著差异。育苗基质以无菌生红土为好 ,生长激素以 1g/L桉树生长调节剂和 1g/L吲哚丁酸为好 ,插条的生根率达 90 %以上。本项技术已在元江大批量培育尾叶桉扦插苗中应用 ,且扦插苗种植后生长快 ,表明尾叶桉在元江干热河谷区是一种值得推广的好树种。     

1年生樟子松苗营养积累过程的研究

通过 3a对樟子松 1年生苗木的营养积累过程的研究表明 :1年生樟子松苗在生长期内 ,干物质的积累和对N、P、K的吸收利用是以叶为中心 ,到生长中、后期 ,根、茎、叶中的干物质积累和对三元素的吸收积累速率明显加快。而在不同生长时期对三元素的吸收利用比例也不同 ,生长前期表现为N >P >K ,生长中后期表现为K >N >P ,生长后期表现为P >N >K。     

西南桦种子贮藏试验

本研究设置干燥、布袋与保鲜袋包装以及系列温度处理，开展了3a西南桦种子贮藏试验。结果表明：布袋与保鲜袋处理对西南桦种子贮藏影响极不影响；在常温常规条件下，西南桦种子贮藏3个月即失去生活力，而在常温干燥条件下，西南桦种子贮藏10个月种子发芽率尚未显著下降；在15℃条件下贮藏10个月几乎丧失发芽能力，非常有趣的，西南桦种子在10、5、0℃和－5℃各温度条件下贮藏3a，效果十分理想，10℃似乎是西南桦种子低温贮藏的临界温度。本研究促进了西南桦的扩大栽培和基因资源的保存。     

稻草编织物覆盖对坡耕地红壤水土流失的影响

通过自然降雨条件下的径流小区试验,研究了水稻编织物在坡耕地红壤水土流失控制上的作用.结果表明:稻草编织物覆盖能有效地减少水土流失,稻草编织物覆盖的小区径流量为697.67 m3,侵蚀量为6.44 t/hm2,而无覆盖的小区产生径流量为1 728.19 m3,侵蚀量为111.98 t/hm2,径流量是覆盖处理的2.48倍,侵蚀量是覆盖处理的17倍.降雨因子与径流量及侵蚀量的关系研究表明:雨强越大,径流量就越大;雨强大也导致侵蚀量增加;侵蚀量不仅与径流量有关,还与人类活动相关.     

不同优质粳稻花药对低温和激素处理的培养响应

以3份优质粳稻品种为供试材料,研究了3种低温预处理和9个激素处理组合对离体花药培养的影响。结果表明:供试材料花药对低温预处理的响应存在明显差异,对激素处理组合的响应也存在一定差异。     

Construction and evaluation of introgression lines and fine mapping of ehd8 from Jinghong common wild rice (Oryza rufipogon)

Heading date is one of the most important traits in rice and regulated by multiple genes. Common wild rice is the ancestor of Asian cultivated rice and harbours abundant genetic diversity. To use wild rice resource in rice breeding, a set of 154 introgression lines (ILs) covering 93% of the genome of Jinghong common wild rice was constructed in the background 'Teqing', using 208 simple sequence repeat markers evenly distributed on 12 chromosomes. Among the ILs, the line JIL64 displayed late heading independent of photoperiod. Genetic analysis using the two F₂ populations crossed ''Teqing'/JIL64 and JIL64/'Teqing' revealed that late flowering was controlled by a recessive gene on chromosome 8 (designated early heading date 8, ehd8), and ehd8 was fine mapped to the 50‐kb region flanked by markers RM22221 and 64Indel4. Sequencing and qRT‐PCR demonstrated that LOC_Os08g01410 and LOC_Os08g01420 were deleted in JIL64 and may be associated with the late heading of Jinghong common wild rice. These findings lay a practical foundation for characterizing ehd8, and the ILs help to mine genes from Jinghong common wild rice.     

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Seed longevity is a very important characteristic controlling seed quality. However, the mechanism underlying this characteristic is poorly understood. In this study, 'FH7185', a storable rice variety, the germination rate of which was 66.63%, much higher than the control after artificial ageing under relative humidity 88% and 42°C for 21 days. Different methods were applied to reveal the involvement of proteins during ageing of rice seeds. In total, 35 differential proteins were identified from 2D‐PAGE, and 3,719 proteins from iTRAQ analysis. A comparison of these two methods showed that not all protein types could be detected on the 2D‐PAGE gels, and the dynamic range was somewhat limited. So the comprehensive proteome map from the iTRAQ analysis was used to identify the quantitatively regulated proteins that played roles in seed longevity, and found that the most marked change was the increased abundance of many metabolic enzymes, especially the ones involved in α‐linolenic acid metabolism in the embryos during ageing. OsLOX2 and OsLOX3 had a negative effect on seed longevity, which were lower in FH7185 than the control. The dehydrogenase (LOC_Os07g44430) which played a major catalytic role in lipid peroxidation, also changed significantly during ageing. Therefore, OsLOX2, OsLOX3 and Loc_OS 07G44430 may be involved in the regulation of seed longevity with a synergistic effect.     

Marker‐assisted breeding for improvement of anaerobic germination in japonica rice (Oryza sativa)

Direct seeding is increasingly being practiced in rice cultivation areas because it saves labour and reduces cost of production and management. In this study, four japonica‐type breeding lines were developed from a cross of a japonica cultivar and an AG‐tolerant donor, KHO, using marker‐assisted selection. Validation of the target genes AG1 and AG2 in the selected lines was examined by PCR analysis and the background recovery rate by the recurrent parent was confirmed by genotyping using a 6K SNP chip. Compared to the recurrent, the survival rates of the selected lines were improved in a range from 33% to 115% in three conditions; in particular, the survival rate was increased in more than twice that of the recurrent parent under the hypoxic condition. The main agronomic trait, yield and quality‐related traits were similar to those of the recurrent parent. The use of these lines will contribute to the expansion of the cultivation area due to the proven economic benefits of direct seeding in Korea.     

Identification and verification of quantitative trait loci for eating and cooking quality of rice (Oryza sativa)

This study was conducted to identify new quantitative trait loci (QTLs) that have stable effects for eating and cooking quality (ECQ) of rice. Three recombinant inbred line populations of indica rice were each planted in two years. Three traits for ECQ, amylose content (AC), gel consistency (GC) and alkali spreading value (ASV), were measured for QTL analysis. A total of 13 QTLs were detected, including four for AC, six for ASV and three for GC. Two QTLs, qGC4 in the interval RM16252–RM335 on the short arm of chromosome 4 and qGC6.2 in the Alk region, were validated in a population derived from a residual heterozygote that was homozygous at the major locus Wx. In the absence of segregation at the Wx locus, qGC4 and qGC6.2 had additive effects of 2.46 and 8.18 mm, respectively, offering a potential for improving GC property of rice varieties. Comparison between qGC4 and previous results suggests that qGC4 is likely a new QTL for GC, providing a candidate for gene cloning and functional characterization.     

气候变化对中国水稻生产的影响研究进展

水稻生产系统是响应气候变化最敏感的农业生态系统之一,本文综述了当前和未来气候变化对我国水稻生产的影响。气候变化背景下,我国水稻生长季的热量资源增多,辐射资源减少,降水不均一性加大。高温热害、干旱、暴雨和洪涝灾害发生更频繁,这可能降低水、热资源的有效性。气候变化使我国单季稻和双季稻潜在种植边界显著北移,导致单季稻、早稻和晚稻的主要生育期缩短。基于统计模型和水稻生长模型的研究结果表明,如果不考虑品种改良和栽培技术的进步,气候变化使单季稻、早稻和晚稻产量下降,但不同稻作区和方法间存在差异。我国水稻生产重心北移、实测生育期延长和产量增加的变化趋势,反映了水稻生产系统通过种植分布调整、品种改良和技术改进来适应气候变化的能力。未来气候变化将进一步导致水稻生育期缩短和产量下降,对我国水稻生产和粮食安全带来严峻挑战。仍需加强气候变化影响机制的研究及其在影响评估中的应用,减小影响评估的不确定性并增加其系统性,为制定有效的应对策略提供可靠的理论支持。