Periodicity and gradients in dispersal ofAlternaria linicola in linseed crops

Conidia ofAlternaria linicola produced on infected linseed crops were mainly dispersed by wind. The numbers of conidia in the air above linseed crops collected by a Burkard spore sampler were greatest between 1200 h and 1300 h, when the relative humidity was lowest. Although numbers of conidia collected decreased with increasing height within and above the crop canopy, air-borneA. linicola conidia were present up to 80 cm above the crop canopy. Conidia ofA. linicola were transported by wind up to at least 40 m downwind from an artificial line inoculum source, but their numbers decreased with increasing distance from the source. In 1991, 1992, and 1993, the dispersal ofA. linicola conidia above linseed crops followed a seasonal periodicity which was influenced by weather conditions and cultural practices. The greatest numbers of conidia were collected during July, August and early September and coincided with periods favourable for sporulation and with an increase in the incidence of the disease in the senescent crop. Air-borneA. linicola conidia produced on point or line inoculum sources (naturally infected linseed stem debris) were responsible for the spread of the disease in linseed crops. In 1992 and 1993, the disease was first detected downwind from the sources, but by the end of the growing seasons, it had spread in all directions and up to 20 m and 60 m from the sources, respectively. Disease gradients were initially steep near the inoculum sources but they became flatter with time due to the secondary spread of the disease.     

Linking habitat selection, emigration and population dynamics of freshwater fishes: a synthesis of ideas and approaches

Abstract –  The consequences of individual behaviour to dynamics of populations has been a critical question in fish ecology, but linking the two has proven difficult. A modification of Sale's habitat selection model provides a conceptual linkage for relating resource availability and individual habitat selection to exploratory behaviour, emigration and population-level responses. Whole-population experiments with pupfish Cyprinodon macularius that linked all factors along this resource to population continuum lend support to this conceptual model, and illustrate that emigration may be much more common in fish populations than considered in most individual- or population-based models. Accommodating emigration can enhance the ecological appropriateness of behavioural experiments and increase confidence in extrapolation of experimental observations to population-level effects. New experimental designs and advancing technologies offer avenues for assessing population consequences of habitat selection and emigration by individual fish. Emigration often is the key linkage between individual behaviour and population responses, and greater understanding of the underlying factors affecting this often-overlooked demographic parameter could offer new approaches for management and conservation of fishes.     

Pollen dispersal in a hinoki (Chamaecyparis obtusa) seed orchard detected using a chloroplast DNA marker

Pollen dispersal was estimated in two test plots in a hinoki (Chamaecyparis obtusa) seed orchard using a chloroplast DNA marker, the spacer region between thetrnD andtrnY genes, and SSCP (single strand conformation polymorphism). In Plot 1, 2,020 seeds from 40 trees within 30 m of the marker tree were analyzed using the PCR-SSCP method. In Plot 2, 1,850 seeds from 37 trees were analyzed in the same manner. The results revealed that the maximum pollen dispersal distance in the two plots exceeded 25 m. Pollen dispersal appeared to be inversely proportional to the distance from the marker tree. The effective pollen dispersal was suggested to be less than about 20 m in a mature hinoki seed orchard. Adjacent trees had an excessive influence when the pollen density was increased by artificial flower stimulation. Therefore, it was suggested that seed production better resembles ideal random mating when carried out as naturally as possible. In conclusion, the SSCP chloroplast DNA marker was a useful tool for amassing basic information on pollen management in seed orchards of coniferous species.     

The horizontal transfer of Salmonella between the lesser mealworm (Alphitobius diaperinus) and poultry manure

There is need to determine the nature of enduring reservoirs of Salmonella contributing to perpetual contamination within poultry flocks. The dispersal of Salmonella between birds, litter and the lesser mealworm has been established, but the extent that these act as critical components in the epidemiology of Salmonella infection during broiler grow‐out and flock rotation has not been delineated; in particular, the level of participation by the lesser mealworm beetles (LMB) as agents of retention and dispersal. This study defines this route of transmission and provides empirical data on bacterial loads that facilitate Salmonella transfer. Results showed differential Salmonella transfer dependent on bacterial concentration. At 10³ cfu/ml, only a small, but not significant, amount of Salmonella was transferred, from the LMB to the manure and back to uninfected LMB; while from 10⁵ to 10⁷ cfu/ml, a significant acquisition and transfer occurred both internally and externally to the LMB over 4 and 24 hr exposures. These data will be used in correlation with facility management practices to develop intervention strategies to mitigate the establishment and spreading of reservoir Salmonella populations contributing to pre‐harvest contamination of poultry flocks.     

Roads limit of seed dispersal and seedling recruitment of Quercus chenii in an urban hillside forest

Paved roads in urban forests cause forest fragmentation and thus reduce animal populations, threaten dispersal and recruitment of animal-dispersed plants. Here, we tracked animal-mediated seed dispersal of Quercus chenii in the urban forest centre and near roadsides to test the effect of paved roads on seed dispersal and recruitment. We conducted experiments in a forest patch in urban areas of Wuhan, China. The forest was dominated by the focal tree species and separated by paved roads. Only two potential seed dispersal animals, one rodent (Niviventer confucianus) and one jay (Garrulus glandarius) were observed in this stand. Seeds were removed farther but not as fast in the forest centre compared to roadsides, while no differences of seed fate, dispersal direction, and seedling establishment were detected between the forest centre and roadsides, indicating the thick vegetation along roadsides provided feeding shelters for small animals. However, when compared with primary forests containing more seed dispersers, seed removal rate in this urban forest fragment was much slower in both forest centre and roadside. Furthermore, due to road barriers, seeds were seldom moved across the paved road and remained in the fragmented stand, reflecting a limited function of seed dispersers. These results suggested paved roads isolated forests into small “islands” and weakened the ecological function of seed dispersers by impeding long distance seed dispersal. Reintroducing dispersers and building wildlife corridors would be impactful ways to restore urban forest patches.     

Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation

Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy (spw1-cls) and determined its molecular genetic mechanism. In the present study, we cultivated spw1-cls over five years to examine effects of cleistogamy on agronomic traits. Simultaneously, we cultivated cleistogamous backcross lines created by continuous backcrossing with “Yumeaoba” (a japonica cultivar) as the recurrent parent and by application of a DNA marker. In these experimental cultivations, spw1-cls and its backcross lines showed almost equal or slightly lower, but acceptable, agronomic traits compared with each control line. We also conducted natural crossing tests in paddy fields to assess the gene containment capability of spw1-cls. In a series of field experiments, there was no natural crossing between spw1-cls (pollen donor) and pollen recipient lines, but the wild-type donor and recipient lines were crossed. Thus, the cleistogamy of the spw1-cls mutation is able to inhibit natural crossing effectively, without significant loss of commercial benefits, such as yield. We conclude that spw1-cls cleistogamy is a practical tool for gene containment in GM rice cultivation.     

Edge Effect on Acorn Removal and Oak Seedling Survival in Mexican Lower Montane Forest Fragments

Vegetation structure of forest edges and type of adjacent field can affect seed and seedling survival patterns of tree species. We investigated acorn removal and seedling survival of Quercus germana and Q. xalapensis in relation to woody plant density across old field-forest gradients with soft and abrupt edges. Experiments were established along four parallel bands located at 40–50 m in the forest, forest and field edges (0–10 m to each side of the border), and 20–30 m in the old field. Within each band, woody plant and acorn density was measured, and four points for acorn and seedling removal experiments were randomly positioned. In each position, 20 acorns or four seedlings were placed. Survival was monitored during one month. In abrupt edges, acorn density was higher along edges than in forest interior. Also, higher acorn removal and seedling mortality were observed in the adjacent old-field whereas acorn and seedling survival was the highest at the edges. Acorn and seedling survival was positively correlated to woody vegetation density. As the vegetation density of field and forest edges grows, abrupt edges develop into soft edges, and they become more hospitable to oak recruitment and then to forest cover expansion.     

日本五针松感染松材线虫病的检测与扩散风险分析

【目的】从日本五针松(Pinus parviflora)栽培圃地调查松褐天牛(Monochumus alternatus),并取样镜检松材线虫(Bursaphelenchus xylophilus),揭示日本五针松感染松材线虫病的可能性和扩散风险,为开展检疫提供理论依据。【方法】在日本五针松圃地采集枯死或濒死的日本五针松植株,采用分离镜检法检测枯死或濒死日本五针松木质部的线虫种类;剖木检查天牛危害,观察留在日本五针松松枝上的补充营养伤口,同时鉴定天牛幼虫特征,解剖检验羽化的天牛成虫携带LⅣ状态。【结果】在枯死或濒死6株日本五针松枝条样本中有3株样本分离到松材线虫或拟松材线虫,检出率为50%,其中1个样本检出纯松材线虫,1个样本检出纯拟松材线虫,1个样本为松材线虫和拟松材线虫混合寄生,检出率各为33%。侵入枝干的天牛幼虫为松褐天牛幼虫,该幼虫能在日本五针松上顺利羽化为成虫,并携带松材线虫L_(Ⅳ)型耐久型幼虫。【结论】日本五针松能被携带松材线虫的松褐天牛危害并感染松材线虫病,应加强检疫管理,杜绝通过人为调运苗木扩散传播松材线虫病。     

Gene dispersal within forest tree populations

Patterns of gene dispersal by seeds and pollen greatly influence the genetic structure of plant populations and their effective size. This paper reviews methods of measuring gene dispersal and current information on patterns of dispersal within local populations of forest trees. Recently, a number of statistical procedures for investigating gene movement based on the use of large numbers of isozyme loci have been described. These procedures include various forms of parentage analysis and the fitting of mating models to genotypic arrays of offspring from individual maternal plants. With the levels of genetic discrimination currently possible in forest trees, the model approach appears to be the most reliable means of estimating gene dispersal parameters. Too little data are available to draw general conclusions about patterns of gene movement within natural populations. Nevertheless, reports to date indicate that dispersal by both pollen and seed can be considerable. For any one mother tree, the bulk of effective pollen in conifers may come from distant males in the same stand or from surrounding stands (gene flow). In insect-pollinated angiosperms, gene flow may also be substantial, but cross-fertilization within stands may primarily be between nearest flowering trees.