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101.
Differences of hyphal and soil phosphatase activities between mycorrhizal and non-mycorrhizal plants were less studied under drought-stressed (DS) conditions. In a pot experiment, fungal alkaline phosphatase (FALP), and succinate dehydrogenase (FSDH), soil phosphatase activity, both soil and plant P contents were compared in 6.5-month-old trifoliate orange (Poncirus trifoliata) seedlings under 80 days of DS with or without inoculations by arbuscular mycorrhizal fungi (AMF, Glomus diaphanum, Glomus mosseae or Glomus versiforme). Plant growth and biomass production under DS were significantly higher in mycorrhizal than in non-mycorrhizal seedlings. Both the FALP and the FSDH activities under DS were significantly reduced in these three Glomus inoculated seedlings. In general, similar soil neutral and alkaline phosphatase activities, but significantly higher soil acid and total phosphatase activities, were exhibited in mycorrhizal than in non-mycorrhizal seedlings under both the well-watered (WW) and the DS. Both leaf and root P contents were significantly higher in the AM colonized seedlings, but soil available P contents were lower in the growth media with AM seedlings. Our results showed that higher hyphal enzymes’ activities, soil acid and total phosphatase activities, and plant P contents in AM colonized seedlings, particularly in Glomus mosseae-colonized seedlings and/or under DS, would result in a better growth of the host plants, which might be the basis for enhancing drought tolerance in plants. 相似文献
102.
首次报道外生菌根菌的共生培养法初步研究了一个较好的外生菌根菌菌丝体培养方法,即将宿主的愈伤组织与外生菌根共同培养,可以明显地促进真菌菌丝的萌发和生长。愈伤组织匀浆液也可促进菌丝体早期生长。 相似文献
103.
Samantha M. Norris Paula A. Schaffer Noah B. Bander 《Journal of veterinary diagnostic investigation》2021,33(5):975
A 15-y-old castrated male Maine Coon cat was evaluated for an ulcerated soft tissue mass on the right hindlimb that had been observed for 4 mo and had grown rapidly. A 3 × 3 cm soft, raised, amorphous, and ulcerated subcutaneous mass was observed on the lateral right metatarsus. In-house cytology via fine-needle aspiration was nondiagnostic. Incisional biopsy of the mass and further staging was declined, and amputation was elected. The amputated limb was submitted for histopathology, which revealed severe chronic nodular granulomatous dermatitis and multifocal granulomatous popliteal lymphadenitis with large numbers of intralesional fungal hyphae. Fungal PCR and sequencing on formalin-fixed, paraffin-embedded tissue identified Chalastospora gossypii. No adjunctive therapy was elected at the time. The patient has done well clinically 1 y post-operatively. C. gossypii is a rare microfungus found worldwide and is considered a minor pathogen of several plants. To our knowledge, infection by this fungus has not been reported previously in veterinary species. Features in our case are comparable to other mycotic infections. Nodular granulomatous mycotic dermatitis and cellulitis, although uncommon, should be a differential for soft tissue masses in veterinary species; C. gossypii is a novel isolate. 相似文献
104.
The unique capacity of fungi to efficiently sequester carbon in aerobic conditions, presents a way to maximize OC gain in agricultural systems. Oat (Avena sativa) was planted in the temperate climate of southern Ontario, Canada to study factors affecting soil organic carbon (OC). The plots varied with initial OC from 25 to 68 g kg−1 or with ground cover of differing decomposability (alfalfa (Medicago sativa) growing from seed, dried oat straw, dried hay and compost) on high OC soil (60–70 g kg−1). The soil was analysed for correlation of changes in soil aggregation, moisture, OC, fungal hyphal number and length and distribution of organic matter by mass and OC in density fractions within the growing season. At harvest, soil OC and moisture were increased only in plots with ground cover. Total hyphal length was not significantly different with ground cover treatment at harvest, and did not correlate with soil aggregation and soil OC. However, the number of hyphae with >5 μm diameter (primarily mycorrhizal fungi) correlated with % OC in ground cover plots while the number of hyphae <5 μm (primarily saprophytic fungi) correlated with % OC without ground cover in the gradient of initial soil OC. Mycorrhizal hyphae may be important to the increases in soil OC from surface treatment, although there was no treatment effect of mycorrhizal occurrence on the oat roots. This microcosm study, with growing and dried ground cover, suggests surface management may a simple and inexpensive means in agriculture to increase soil moisture and OC that benefits farmers as well as reducing atmospheric CO2. 相似文献
105.
This study compared genomic predictions using conventional estimated breeding values (EBV) and daughter yield deviations (DYD) as response variables based on simulated data. Eight scenarios were simulated in regard to heritability (0.05 and 0.30), number of daughters per sire (30, 100, and unequal numbers with an average of 100 per sire) and numbers of genotyped sires (all or half of sires were genotyped). The simulated genome had a length of 1200 cM with 15,000 equally spaced Single-nucleotide polymorphism (SNP) markers and 500 randomly distributed Quantitative trait locus (QTL). In the simulated scenarios, the EBV approach was as effective as or slightly better than the DYD approach at predicting breeding value, dependent on simulated scenarios and statistical models. Applying a Bayesian common prior model (the same prior distribution of marker effect variance) and a linear mixed model (GBLUP), the EBV and DYD approaches provided similar genomic estimated breeding value (GEBV) reliabilities, except for scenarios with unequal numbers of daughters and half of sires without genotype, for which the EBV approach was superior to the DYD approach (by 1.2 and 2.4%). Using a Bayesian mixture prior model (mixture prior distribution of marker effect variance), the EBV approach resulted in slightly higher reliabilities of GEBV than the DYD approach (by 0.3-3.6% with an average of 1.9%), and more obvious in scenarios with low heritability, small or unequal numbers of daughters, and half of sires without genotype. Moreover, the results showed that the correlation between GEBV and conventional parent average (PA) was lower (corresponding to a relatively larger gain by including PA) when using the DYD approach than when using the EBV approach. Consequently, the two approaches led to similar reliability of an index combining GEBV and PA in most scenarios. These results indicate that EBV can be used as an alternative response variable for genomic prediction. 相似文献