萍乡采矿废弃区退化森林的恢复与重建技术

借鉴国内外采矿废弃区退化森林的恢复重建经验,阐述了萍乡采矿废弃区退化森林的恢复、重建技术,并总结提出了萍乡采矿废弃区退化森林的恢复、重建的措施。     

Real-time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine: comparison with conventional PCR detection and application in diagnostics

A new real-time PCR detection system was developed for grapevine yellows (GY) using TaqMan minor groove binder probes and including two amplicons for group-specific detection of Flavescence dorée (FD) and Bois noir (BN) phytoplasmas, plus a universal phytoplasma amplicon. FD and BN amplicons were designed to amplify species-specific genomic DNA fragments and the universal amplicon to amplify the 16S ribosomal DNA region. Efficiency of PCR amplification, limit of detection, range of linearity and dynamic range were assessed for all three amplicons. The specificity of detection systems was tested on several other isolates of phytoplasmas and bacteria and on healthy field grapevine and insect samples. No cross-reactivity with other phytoplasma strains, plant or insect DNA was detected. The assay was compared with conventional PCR on more than 150 field grapevine, insect and field bindweed samples. Real-time PCR showed higher sensitivity as phytoplasmas were detected in several PCR-negative and in all PCR-positive samples. A data-mining analysis of results from both detection approaches also favoured real-time PCR over conventional PCR diagnostics. The developed procedure for detection of phytoplasmas in grapevine also included amplification of plant DNA co-extracted with phytoplasmic DNA, providing additional quality control for the DNA extraction and PCR amplification for each sample. The newly developed assay is a reliable, specific and sensitive method easily applicable to high-throughput diagnosis of GY.     

平原地区采煤塌陷对土地的影响及复垦治理

阐述了平原煤矿区采煤塌陷对土地自然形态、土壤结构、地下水位等方面的影响,论述了重建矿区土地生态系统的意义,并提出了重建矿区土地生态系统的几项措施。     

贵州锰矿废渣堆场重金属污染风险评价及草本植物重金属吸收特征

为明确锰矿废渣堆场重金属污染及优势草本植物重金属吸收特征,对贵州省典型锰矿废渣堆场的重金属污染风险进行了评价,并调查研究了锰矿废渣堆场草本植物的类群及重金属吸收特征.共采集优势草本植物18种,隶属11科18属,菊科和禾本科为优势科.对18种草本植物体地上部、地下部及其生长基质中Cr、Ni、Cu、Zn、Cd、Pb和Mn ...     

一种水窖入水口的自闭系统初探

在对水窖较为普遍的窖体破坏、塌窖等原因进行分析的基础上,引入一种防止水窖超蓄塌陷的水窖入水口自闭系统.此系统结构简单、造价低、使用方便,能及时自闭入水口且关闭效果好,保证水窖蓄水不超过限制水位,从而保障水窖的结构稳定,延长水窖的使用年限.     

排矸区沙棘复垦及沙棘果深加工的研究

从4个方面分别对鹤岗矿区矸石排放区栽植沙棘的技术、利用价值、深加工技术流程及其生态效益和社会效益做了介绍.     

基于脆弱性理论东北地区典型煤炭城市社会系统研究

从社会系统脆弱性理论入手，找出了灾害框架下和煤炭城市框架下社会脆弱性共性和个性。借助危险、灾害和脆弱性的关系，做出典型煤炭城市危险机制图。选定指标以后，利用层次分析法确定权重，进行胁迫现状评估和危险评估，最后做综舍分析。     

前工业化时期英国地主投资工业研究

16至18世纪是英国由农业社会向工业社会转型的时期，地主阶级中的一部分人顺应了商品经济发展的要求，为攫取更多利润，他们改变原有土地经营方式，在地产的基础上从事矿业和交通运输业，从而为工业革命创造了必要的物质条件。从这个意义讲，地主是英国早期工业发展的“革新者”、“赞助者”、“投资者”和“开拓者”。     

城市燃气管网安全运行综合技术

论述了开展城市燃气管网安全运行综合技术研究的意义，对保证管网安全运行的关键技术进行了综合介绍。面对城市管网安全运行的严重问题，建议应尽快开展城市燃气管网安全运行综合技术研究，尽快建立城市燃气管网安全运行的综合技术系统。     

钻孔灌注桩施工事故预防及处理的几点体会

文章介绍了在砂砾土质钻孔塌孔的预防及处理方法，还介绍了首罐砼灌注及断桩处理方法。