A Case of Spontaneous Malignant Hibernoma in a Crl:CD(SD)IGS Rat

A firm, tan, well-circumscribed mass that measured 25 × 30 × 35 mm was observed in the thoracic cavity of a 53-week-old male Crl:CD(SD) IGS rat. Histologically, the mass was encapsulated by fibrous tissue and contained fibrovascular septae. Tumor cells were compactly arranged, and most were oval to polygonal in shape with multivacuolated cytoplasm and a centrally located nucleus. In some parts of the tumor, marked cellular atypia and frequent mitoses were evident. Vacuoles in cytoplasm were positive for oil red O. The tumor cells were characterized ultrastructurally by abundant, round to oval mitochondria with transverse closely-packed cristae. Tumor cells were immunohistochemically positive for uncoupling protein 1 (UCP-1). Several thrombi and hemorrhagic or necrotic foci were also observed within the tumor mass. Vascular invasion of the tumor capsule was observed; however, invasion of surrounding tissues or metastases were not observed. Based on the pathology findings, this case was diagnosed as a malignant hibernoma.     

猪胎儿成纤维细胞SOD1基因差异表达研究

 在研究模式生物衰老机制的过程中,发现衰老受某些特定基因的调控。为研究细胞内铜锌超氧化物歧化酶(Cu/ZnSOD,SOD1) 在不同代数的猪胎儿成纤维细胞的差异表达,本试验收集正常传代生长的5,10,15,20,25,30,35,40,45,50,55,60,65,70代的猪胎儿成纤维细胞,分别提取细胞总RNA;采用RT-PCR技术,检测不同代数猪胎儿成纤维细胞SOD1的表达。结果表明猪SOD1在第5代猪胎儿成纤维细胞中表达水平最高,随着细胞代数的增加,表达量逐渐下降,到第70代表达量为最低。结果将为进一步揭示细胞衰老的分子机制提供有价值的参考,同时为丰富猪的分子生物学提供新素材。     

脑炎原虫病兔免疫器官的病理形态学观察

研究脑炎原虫病兔外周免疫器官的形态学变化,采集20只病兔和12只对照兔的淋巴结、脾脏和圆小囊,通过临床检查,病理剖检,病理切片的HE染色、吉姆萨染色和免疫组织化学染色进行了比较观察。结果表明病兔淋巴结内的淋巴小结数量增多,生发中心明显,髓索增粗,副皮质区增宽。脾脏的脾小结增多,变大,具有明显的生发中心,边缘区明显增宽,动脉周围淋巴鞘明显变大,聚集大量淋巴细胞。圆小囊的淋巴小结增大,数量增多,生发中心明显,淋巴小结周围弥散大量淋巴细胞。通过α-ANAE和sABC染色,在淋巴结、脾脏和圆小囊中均可检出大量T细胞、巨噬细胞和IgG阳性细胞。据此认为患脑炎原虫病时,病兔的体液免疫和细胞免疫均增强,但以细胞免疫反应更强。     

Immunophenotyping of Leukocyte Subsets in Peripheral Blood and Palatine Tonsils of Prefattening Pigs

The quantitative and distribution patterns of porcine peripheral blood and tonsillar lymphoid/myeloid cell subsets were assessed in order to establish the immune status of farm pigs prior to their transfer to fattening units. Peripheral blood and tonsillar samples were taken from clinically healthy, nonvaccinated, 12-week-old pigs, either ex vivo or following euthanasia. Single-colour flow cytometry, using monoclonal antibodies (mAbs) reactive with the swine leukocyte cluster of differentiation (CD) antigens, gave the proportions of lymphoid (9.7% CD4⁺, 8.0% CD8⁺, 36.9% CD5a⁺, 20.3% CD16⁺, 6.9% CD21⁺, 86.3% CD45⁺, 41.8% CD45RA⁺, 48.3% CD45RC⁺), null cells (6.9%) and myeloid cells (23.7% CD11b⁺ and 5.4% SWC3a⁺) in peripheral blood. In situ identification and distribution of lymphoid cells in the tonsils (CD3a⁺, CD21⁺, CD45RA⁺, CD45RC⁺) was performed with anti-CD mAbs using the avidin–biotin complex method. Most CD3a⁺ cells were in the parafollicular areas, with many cells in the follicles. CD21⁺ cells were scattered throughout the parafollicular area, with only a few cells inside lymphoid follicles. CD45RA⁺ cells were mostly concentrated in the follicles but many positive cells were present in the parafollicular area. Many CD45RC⁺ cells were visible in the parafollicular area, a few positive cells were in the crypt epithelium, and single cells were inside the follicles.     

KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media

Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco''s modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.     

元胞自动机模型在森林景观生态规划中的应用

元胞自动机是有时空特征的离散型动力学模型,具有模拟复杂系统时空演化过程的能力,对于有空间特征的森林空间结构模拟及景观生态规划具有极大的应用价值。运用GIS描述和处理静态空间信息,元胞自动机模型(CA)则为动态的模型,CA与GIS集成将极大促进GIS对森林景观过程的模拟能力。文章论述了用元胞自动机模型进行森林景观生态规划的基本构想。     

高压脉冲电场处理果蔬元胞自动机模拟研究

研究旨在利用具有时空特征的元胞自动机动力学模型对高压脉冲电场处理的果蔬介质进行仿真,模拟果蔬介质计算过程,为高压脉冲电场技术在果蔬保鲜、干燥以及杀菌中的应用提供理论依据。在考虑高压脉冲电场处理果蔬产生细胞电击穿现象以及电击穿原理的基础上,通过元胞空间、状态和规则的制定,构建了元胞自动机果蔬介质击穿模型。结果表明,该试验的模拟结果符合高压脉冲处理果蔬介质的击穿特征,与实际情况对比拟合度高,误差较小。利用元胞自动机仿真高压脉冲电场处理果蔬介质模型,反映了果蔬介质的击穿过程,有利于高压脉冲电场技术在果蔬领域的应用及推广。     

Supplementing the feed of pikeperch [Sander lucioperca (L.)] juveniles with MacroGard and its influence on nonspecific cellular and humoral defense mechanisms

This study examined the influence of β‐1.3/1.6‐glucan (MacroGard) on the innate immune system in healthy pikeperch (Sander lucioperca) juveniles. MacroGard was fed in a pelleted ration of 1 or 2 g kg^?1 feed for 6 weeks. The control group of fish was fed feed with no supplement. Blood, pronephros and spleen samples for the study of nonspecific cellular and humoral defense mechanisms were collected from 10 fish from each group. No changes were observed in either the behaviour or the health of the fish throughout the rearing period. Fish mortality was not noted in any of the groups. Additionally, the supplementation of feed with MacroGard did not have a significant impact on the fish growth rate as expressed in both absolute and relative values (P>0.05). The condition of the fish from the experimental group did not differ from that of the control group specimens, and the feed conversion ratios were also very similar (P>0.05). The results showed that MacroGard administered in two doses significantly (P<0.05) activated the metabolic activity and potential killing activity of spleen phagocytes, compared with the control‐fed pikeperch. The highest phagocyte activity was observed with a dose of 2 g kg^?1. The proliferative response of pronephros lymphocytes indicated a similar pattern. MacroGard significantly increased proliferative response of lymphocytes stimulated by mitogens (P<0.05). MacroGard also significantly increased the lysozyme activity and total immunoglobulin levels in the serum. In this study neither significantly different ceruloplasmine activity or total protein level in serum were observed. The result showed that MacroGard at the optimal dose of 1 g kg^?1 feed and a twofold higher dose does not have a negative influence on the hepatocytes or increased nonspecific cell‐ and humoral‐mediated immunity in healthy pikeperch.     

甲壳动物细胞及体液免疫机理的研究进展

根据国内外的有关资料,对甲壳动物细胞及全液免疫进行了简要的评述，其机体的免疫防御功能主要依赖于血细胞的吞噬，包掩等细胞免疫反应，但目前对于血细胞的分类及各自的功能尚无定论，甲壳动物体液吵具有免疫球蛋白，缺乏抗体介导的免疫反应，其体液免疫主要是指血淋巴中的一些酶和调节因子，如酚氧化酶原激活系统，落血素，凝集素，溶菌酶，蛋白酶抑制剂等。     

猪圆环病毒2型Rep蛋白在真核细胞中的表达与定位

为了构建Rep蛋白真核表达质粒，研究PCV2复制过程中的亚细胞分布。本研究借助PCR方法扩增PCV2的Rep基因，并将其亚克隆到真核表达载体pEGFP-C3中，并转染PK15细胞和Vero细胞。结果显示，重组的pEGFP—PCV2-Rep融合蛋白能够在PK15细胞和Vero细胞中表达。结论认为pEGFP—PCV2-Rep质粒转染PK15细胞和Vero细胞后48h，PCV2的Rep蛋白主要定位在PK15细胞和Vero细胞的细胞核。