Biocompatibility of Acellular Porcine Dermis

In vitro cultured vascular endothelial cells (VEC) and mouse 3T3 fibroblasts (3T3) on the acellular dermal matrix , which were made of porcine skins. We made the cell proliferation test with MTT assay and the histological observation after cells were seeded on the acelluar dermis for 1 week with histological section. The cell growth curves showed VEC and 3T3 grew much rapidly on the acelluar dermis.The histological observation revealed VEC had formed a monolayer, some places even had formed 2 to 3 layer. The results suggest that the acelluar porcine dermal matrix have good biocompatibility , it will be widely applied.     

MTT法检测大鼠外周血淋巴细胞增殖反应探讨与应用

为了更精准简捷地评价机体的细胞免疫状态,采用MTT比色分析法检测大鼠外周血淋巴细胞的增殖能力,并对试验的最适条件进行了探讨,然后将该法应用到Transwell共培养体系中以评价H9N2 AIV感染内皮细胞后对跨内皮淋巴细胞增殖能力的影响。结果表明：在96孔细胞培养板中接种密度为1.25×10⁶个/mL的淋巴细胞于含10% FBS的RPMI 1640培养基中培养的最适时间为48 h。感染病毒后,跨内皮淋巴细胞的增殖明显受抑制;内皮完整状态下,淋巴细胞和病毒共孵育后,活病毒的量降低,而当内皮不存在或被病毒感染后,这一现象消失。结果证明作为机体细胞免疫功能评价指标之一的淋巴细胞增殖能力可采用改良后的MTT法检测。     

中药细胞级粉碎及其发展趋势

细胞级粉碎是一项新兴的物料加工技术。介绍了中药细胞级粉碎的概念、原理、特点、设备及其发展趋势。     

新生猪肌组织成纤维细胞的分离培养和鉴定

研究建立了猪肌组织成纤维细胞体外培养的模型,采用组织块培养方法获得了新生猪肌组织成纤维细胞并进行体外培养.观察细胞形态,通过绘制细胞的生长曲线来研究肌组织成纤维细胞的生长规律,利用RT-PCR和免疫细胞化学染色对所获得的细胞进行鉴定,再用双荧光素酶检测系统来检测细胞的转染效率.通过形态学观察、RT-PCR和免疫细胞化学染色结果表明,已成功培养了猪肌组织成纤维细胞,纯度达90%以上.     

逼近水力机械特性的一种新方法

针对现有曲线逼近方法的不足，本文给出一种基于元胞自动机原理的曲线逼近方法，并对应用这种方法逼近水力机械特性的计算过程进行了研究。研究结果表明，使用基于元胞自动机的曲线逼近方法逼近水力机械的特性，与整体的曲线逼近方法相比，所得到的逼近结果，具有计算精度高，能够真实反映水力机械特性的特点，可以用于水力机械的控制和优化运行等方面。     

共轭亚油酸对动物免疫功能的调节

共轭亚油酸具有广泛的生物学功能,它对增加机体免疫球蛋白含量,促进免疫细胞增殖、细胞因子产生、吞噬细胞的功能,基因表达都具有调节作用。本文就共轭亚油酸的免疫调节作用进行了阐述。     

静磁场对人白血病细胞K562 DNA的损伤模式研究

【目的】研究静磁场处理对人白血病细胞DNA的损伤模式与损伤程度,为肿瘤细胞的物理治疗提供依据。【方法】以人白血病细胞K562为试材,对其进行8.8mT静磁场处理6,12,24,30,36h后,采用MTT检测细胞活力并对细胞进行计数,同时联合使用单细胞凝胶电泳以及原子力显微镜观测方法,分析经过静磁场处理后K562细胞DNA的损伤模式。【结果】K562细胞在8.8mT静磁场中处理24h,细胞生长受到抑制,细胞彗星尾长与对照相比有显著差异(P<0.05);处理时间延长到30h,尾部DNA含量和尾长与对照相比均有极显著性差异(P<0.01),表明随着静磁场处理时间的增加,K562细胞DNA的损伤模式与损伤程度发生变化。原子力显微镜观察结果显示,静磁场处理12h,K562细胞DNA已经发生损伤,细胞DNA分子的平均高度增加,平均长度减小;静磁场处理24h,DNA链变短变粗,小片段DNA明显增多,部分DNA发生断裂;静磁场处理36h,细胞DNA形态发生显著变化,DNA大部分断裂成小片段,小片段DNA之间相互交联成板状聚集体。【结论】8.8mT静磁场对K562细胞有杀伤效应,并且这种杀伤作用具有随处理时间延长而累积的效应。随着静磁场处理时间的延长,细胞DNA经历了解链-断裂-交联和断裂并存的变化过程,DNA损伤程度亦逐步加剧。     

Early Morphological and Physiological Events Occurring During Germination of Maize Seeds

The early morphological and physiological events occurring during maize （Zea mays cv. Nongda 108） seed imbibition and germination were studied. Water uptake of seeds exhibited a triphasic pattern with a marked increase during the initial phase of imbibition, and then a slow increase, followed by a second substantial increase. Imbibition time for 10 and 50% of seed germination was about 26 and 46 h at 30℃, respectively. The relative conductivity of maize seeds dramatically decreased during the initial phase of imbibition, followed by a substantial increase. Respiratory rate of seeds gradually increased with imbibition. Length of root cap cells decreased during the initial phase and then increased; those of meristematic zone cells increased during the initial phase and then decreased; and those of elongation zone cells and of the whole elongation zone of the radicle gradually increased during germination. The contents of soluble sugars and starch in embryos gradually decreased as the activities of α- and β-amylase strikingly increased with imbibition. In the meantime, protein contents of embryos gradually decreased and free amino acid content increased. The activities of aminopeptidase and endopeptidase increased until 12 h of imbibition and then decreased. It is concluded that germination of maize seeds is mainly completed by extension of cells in the elongation zone of the radicle, and that mobilization of stored reserves in the embryo during the initial phase of imbibition is also an early event during seed germination.     

8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响

用氯磺酸-吡啶法修饰当归多糖(CAPS)和枸杞多糖(LBPS),得到4种硫酸化当归多糖sCAPS0.6、sCAPS1.1、sCAPS1.9、sCAPS2.2和4种硫酸化枸杞多糖sLBPS0.7、sLBPS1.1、sLBPS1.5和sLBPS1.9。分别以CAPS和LBPS为对照,采用MTT法比较了这8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响。结果表明:硫酸化修饰能显著提高当归多糖和枸杞多糖的抗病毒活性,且与硫酸基取代度有一定的相关性。sCAPS2.2、sLBPS1.5、sCAPS1.9和sLBPS1.9的活性较好,可以作为进一步研究的材料。     

黄河三角洲农业用地动态变化模拟与情景分析

为明确农业用地变化的动态特征和影响要素,预测不同情景下的农业用地的时空变化格局。利用遥感和地理信息系统技术对黄河三角洲地区4期TM和ETM+影像进行解译判读,获取区域内1992－2005年间农业用地动态演化特征,并构建元胞自动机模型对2001年和2005年的农业用地格局进行模拟。2001年和2005年农业用地模拟的总体精度分别为82.90%和84.48%,Kappa系数分别为0.658和0.689。模拟结果表明元胞自动机模型能够较好地模拟研究区域内农业用地动态变化,为农业用地的演变模拟提供了一种适用的工具。利用元胞自动机模型对4种情景下（面积减少5%、增加5%、10%和15%）的农业用地演变进行了预测。情景分析表明刁口故道附近的农业用地并不稳定,容易发生变化,是需要重点关注的区域。