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排序方式: 共有113条查询结果,搜索用时 46 毫秒
51.
一氧化氮合酶(nitricoxidesynthase,NOS)专一性催化L-精氨酸转化为L-瓜氨酸和一氧化氮(nitricoxide,NO),产物NO是一种重要的生物信使分子,对其功能和代谢的研究越来越受人们的重视[1]。国际上,鱼类NOS的研究还刚起步,已有研究者在鱼类中检测到NOS的存在[2-5]。虹鳟[6]、金鱼[4]、大西洋鲑[7]和沟鲶[8]的诱导型NOS(iNOS)和神经型NOS(nNOS)的部分序列已鉴定。国内对哺乳动物的NOS也进行了研究[9-11],尚未见关于鱼类NOS方面的研究报道。JOURNALOFFISHERIESOFCHINA Vol.27,No.4 斜带石斑鱼(Epin… 相似文献
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The occurrence of pre-maturity α-amylase (PMA) is a major cause of poor bread-making quality (low Hagberg Falling Number) in wheat grain. In susceptible genotypes, it involves the excessive accumulation of high isoelectric point (pI) α-amylase in mature grain prior to germination and in the absence of pre-harvest sprouting. Several factors regulate PMA formation in developing grain, including genotype, agronomy, and environmental conditions. In particular, a cold period during mid-grain development has been found to be a major stimulus for PMA induction. Although the factors affecting the PMA occurrence are well known, little is known about the molecular mechanism governing its induction. The plant hormones abscisic acid (ABA) and gibberellins (GAs) influence various aspects of grain development, and it has been suggested that PMA involves changes in the amount of these hormones or the sensitivity of the grain to these hormones. This review summarizes recent studies investigating the role of ABA and GAs in PMA induction and PMA occurrence. 相似文献
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以拟南芥和杨树的钙调蛋白氨基酸序列为探针,对橡胶树转录组数据库进行搜索,并设计引物进行PCR扩增,得到7个橡胶树钙调蛋白基因的c DNA序列,命名为Hb Ca M1-7。序列分析结果发现,Hb Ca M1-7的CDS序列均为450 bp,编码149个氨基酸,分子量为16.8 ku,等电点为3.95。其中,Hb Ca M1-6间氨基酸同源性为100%,Hb Ca M7与其他成员之间的氨基酸同源性为98%。在基因结构组织方面,Hb Ca M1-7均为一个内含子,且内含子的插入位置相同,但内含子的长度明显不同。从氨基酸序列同源性和进化关系分析结果可看出,钙调蛋白基因家族在进化上非常保守。在表达方面,Hb Ca M1-6在各组织中均有表达,除了Hb Ca M3在根中表达丰度相对较高,其他成员均在胶乳中表达丰度相对较高,而Hb Ca M7在各组织中的表达均比较低;乙烯利处理后,Hb Ca M2-7的表达均有一定的上升趋势,而在叶片发育过程中Hb Ca M1-5碷呈明显下调表达。由此可见,橡胶树钙调蛋白与橡胶树叶片发育、胶乳乙烯信号通路具有一定的相关性。 相似文献
57.
结球甘蓝花粉钙调素基因的克隆与表达分析 总被引:1,自引:1,他引:0
根据芸薹属植物钙调素基因保守区域设计引物,采用同源克隆的方法从结球甘蓝自交不亲和系和自交系花粉中克隆得到一个钙调素开放阅读框cDNA 序列,该序列长450 bp,编码149 个氨基酸;编码蛋白不含跨膜区,无信号肽,具有4 个完整的EF-hand 结构域。构建了结球甘蓝花粉钙调素原核表达系统,钙调素基因及其3 个突变体在E. coli 中得到表达,均获得分子量约为16 kD 的可溶性融合蛋白,在EGTA 存在的条件下,各融合蛋白具有各自独特的凝胶迁移现象,活性检测表明甘蓝花粉钙调素活性依赖于钙离子。该基因在甘蓝自交不亲和系花粉萌发过程中表达量先上升后下降,在自交系中随花粉萌发增大而降低;在含有钙调素拮抗剂TFP 的培养基中,自交不亲和系和自交系花粉中钙调素基因表达量均受到抑制;在含有W-7 琼脂糖的培养基中无明显差异。 相似文献
58.
Insect peptide hormones: a selective review of their physiology and potential application for pest control 总被引:2,自引:0,他引:2
Our knowledge on primary structure, synthesis, release, receptor binding, structure-activity relationships, mode of action and degradation of, mainly, neuropeptides from insects has increased dramatically during the last 10 years or so. Here, five case studies are presented, which deal selectively with effects on: reproduction (trypsin modulating oostatic factor in mosquito); energy metabolism, locomotion and the immune system (adipokinetic hormones); water and ion balance, and feeding behaviour (diuretic hormones, kinins, sulfakinins); sex attraction (pheromone biosynthesis activating neuropeptide); and growth and development, and muscle activity (allatostatins). The literature is reviewed in the context of how the knowledge on neuropeptides has been and can be used for the design of novel, safe and selective compounds to control pest insects in the foreseeable future. 相似文献
59.
Lane IF Fischer JR Miller E Grauer GF Lappin MR 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(1):43-49
Three dogs with dysuria and urine retention caused by excessive functional urethral resistance are described. All dogs had clinical histories and urologic signs that previously would have been classified as detrusor-urethral dyssynergia. Diagnosis of functional urinary obstruction was established by exclusion of anatomic urinary obstruction and confirmed by urethral pressure profilometry. In 2 cases, multiple pressure deflections recorded in the urethral pressure profile suggested spasm of urethral musculature, whereas in a 3rd dog, abnormally high pressures were recorded along a portion of the proximal urethra. Functional urinary obstruction was associated with prostatitis in 1 dog and with a history of urethral calculi in 1 dog, and no underlying disorder could be identified in the remaining dog. All 3 dogs improved with medical treatments that included alpha adrenergic antagonists. The etiology, diagnosis, and pharmacologic management of functional urinary obstruction are discussed. 相似文献
60.
Cochliobolus miyabeanus forms a specialized infection structure, an appressorium, to infect rice. Contacting a hard surface induces appressorium
formation in C. miyabeanus, while the hydrophobicity of the substratum does not affect this morphogenic infection event. To determine whether the calcium/calmodulin-dependent
signaling system is involved in prepenetration morphogenesis in C. miyabeanus, the effects of a calcium chelator (ethylene glycol tetraacetic acid; EGTA), phospholipase C inhibitor (neomycin), intracellular
calcium channel blocker (TMB-8), calmodulin antagonists (chlorpromazine, phenoxybenzamine, and W-7), and calcineurin inhibitor
(cyclosporin A) on morphogenesis and infection were examined. Addition of Ca2+ and the calcium ionophore A23187 did not affect conidial germination, while the number of appressoria decreased with higher
concentrations. EGTA inhibited conidial germination and appressorium formation. The calcium channel blocker did not affect
appressorium formation at any concentration; however, calmodulin antagonists and the calcineurin inhibitor specifically reduced
appressorium formation at the micromolar level. One of the calmodulin antagonists, W-7, also inhibited accumulation of mRNA
of the calmodulin gene within germinating conidia and/or appressorium-forming germ tubes. Thus, biochemical processes controlled
by the calcium/calmodulin signaling system seem to be involved in the induction of prepenetration morphogenesis on rice. 相似文献