基于SPR生物传感器的H5N1流感病毒快速检测方法

为利用表面等离子共振(Surface Plasmon Resonance,SPR)生物传感器建立一种快速检测H5N1流感病毒的新方法。该研究利用SPR生物传感器以及H5N1单克隆抗体,对H5N1流感病毒样品进行检测分析。结果表明:该方法特性如下:1)可实现针对H5N1流感病毒的直接、快速和实时检测,整个过程无需任何标记;2)检测限为104.44 TCID50/mL,低于ELISA方法的103.24 TCID50/mL;3)在抗体消耗量方面,SPR技术要优于ELISA技术近100倍。     

生物传感器在食品分析检测中的应用

本文简介了生物传感器的组成构造及工作原理,并对各类生物传感器的特性及在食品分析检测中的应用进行了综述。生物传感器具有较好的敏感性、特异性,操作简便、响应速度快等优势,并可以现场在线检测,在食品分析检测中有巨大的应用潜力。     

生物传感器在农药残留检测中的应用

农药的广泛使用需要可靠的工具对其进行监测,从而保护人类和环境安全。传统的分析方法由于繁琐耗时或仪器的昂贵有其局限性。生物传感器是一种以生物活性单元为敏感元件,结合化学、物理转换元件,对被分析物具有高度选择性的仪器,可以用来检测农药残留等污染物。该文综述了近年来生物传感器在环境、食品和农产品农药残留检测中的研究进展。     

Enzymatic Hydrolysis of Alaska Pollock Proteins Based on Kinetics Model and Lysine Biosensor–Neural Network Model

Controlled proteolysis is important for target hydrolysates. The hydrolysis kinetics of pollock bone protein (PBP) was obtained by mathematic deduction and experimental analysis. The equation of degree of hydrolysis was as follows: DH = 2.801 ln|1 + (0.06044E₀/S₀?0.1005)t|, which could predict the hydrolysis process of PBP when hydrolysis condition was strictly controlled. However, the hydrolysis reaction was affected by several factors, and the predicted value may be against the experimental result. Therefore, online monitoring was necessary for obtaining the target peptides. Biosensor and artificial neural network (ANN) were employed for monitoring hydrolysis process online. Free lysine level was chosen as the monitoring factor determined by immobilized lysine oxidase electrodes. Based on the lysine biosensor and ANN, the hydrolysis monitoring model LYS-ANN was built, including 3 input layer nodes (E₀, S₀, and LYS), 1 output layer node (DH), and 11 hidden layer nodes. R² value between sample value and simulation value was 0.9964. Simulation error was in the range of 0–4.56%, and the average relative error was 0.94%. The verification tests of PBP hydrolysis showed that the LYS-ANN model could forecast hydrolysis process successfully with high efficiency and accuracy even if the hydrolysis conditions varied.     

抗微生物药物残留检测方法研究新进展

抗微生物药物残留检测一直是食品安全监督体系重要的组成部分。为了建立安全、高效、可靠的监测体系,近年来相继出现了一些快速、灵敏、高效的检测方法,极大地促进了抗微生物药物残留检测技术的发展。首先对抗微生物药物残留的安全风险进行了评价,并回顾了近年来抗微生物药物残留检测方法研究的最新进展。最后对生物传感器、色谱-质谱联用、分子印迹聚合物和微阵列芯片检测方法的优缺点进行了比较分析,并对这些技术的发展进行了展望。     

适配体传感器的研究进展

适配体作为传统抗体的替代物,在药物残留检测、临床诊断、靶向治疗等许多领域有广范的应用。适配体与生物传感器检测技术相结合,可研制出一系列适配体传感器。介绍了适配体的概念和优点,适配体传感器的种类,如荧光传感器、电化学传感器、比色传感器、SERS（surface enhanced raman scattering）传感器和SPR（surface plasmon resonance）传感器,以及它们的原理、检测限和应用情况,展望适配体传感器在兽药残留检测和动物疾病诊断等方面的应用前景。     

基于生物传感技术测定植物激素的研究与展望

利用生物传感器定量分析样品中植物激素含量,其具有灵敏度高、操作简便,准确迅速的特点,可以达到原位、实时测定的要求。以电流型免疫生物传感器为例介绍生物传感器在植物激素定量分析领域的应用和发展前景。     

竞争性抑制酶联反应在量热式农残生物传感器中的试验研究

以量热式农残生物传感器为研究对象,借鉴免疫分析方法,进行竞争性抑制酶联反应在量热式农残生物传感器中的试验研究。结果表明,在甲胺磷浓度为0~0.004 mg.kg-1范围内,甲胺磷含量和放热量之间的线性较好,竞争性抑制酶联反应分析法测农药残留的最低检测限为0.0011 mg.kg-1,每次测定时间为20 min。     

天然除虫菊酯检测方法的研究进展

天然除虫菊酯已成为当今无公害生物杀虫剂的研究热点之一,广泛应用于农业、林业、生物农药和食品卫生安全等多个领域。本文总结了目前相关研究的现状与成果,分析了目前应用于检测天然除虫菊酯的薄层色谱法、气相色谱法、液相色谱法及联用技术的优缺点。重点讨论了在天然除虫菊酯残留检测方法中,代表其发展趋势的免疫学方法及生物传感器技术,并在此基础上提出实现检测的设想。     

Comparison of chemical, electrophoretic and in vitro digestion methods for predicting fish meal nutritive quality

Chemical, electrophoretic and in vitro digestion methods were compared with respect to predictions given regarding fish meal (FM) quality. FMs were manufactured by mixing a press-cake, with spray dried stickwater concentrate from the identical raw material, thereby providing samples containing different quantities of water-soluble protein (wsp). A low-temperature-dried FM was employed as a reference. Acquired chemical data for each of the FMs included amino acid analysis and proximal composition (protein, fat, ash, ammonia, titration, salt, moisture). Biological methods in rat (net protein utilization, NPU, biological value, BV, and true digestibility, TD), capillary electrophoresis (sodium dodecyl sulphate-capillary gel electrophoresis, SDS-CGE)) and an in vitro enzymatic assay (trinitrobenzene sulphonic acid-based closed system with rainbow trout enzyme extract) were used for further comparisons with FM wsp content. A high correlation ( R  = 0.97; P  < 0.001) between FM wsp content and titration volume was observed. In contrast to BV and NPU ( R  = 0.98; P  < 0.001), TD ( R  = 0.2; P  = 0.63) did not correlate with FM wsp. The peak area of a 50 kDa signal derived from SDS-CGE showed significant correlation ( R  = 0.98; P  < 0.001) with wsp content. The fish-based in vitro system provided correlations with wsp content with respect to predigestion ( R  = 0.97; P  < 0.0001) and post digestion ( R  = 0.77; P  < 0.03) and for enzymatic liberation of amino groups as post digestion minus predigestion ( R  = 0.97; P  < 0.0001) from the FM examined.