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41.
进行了安普霉素耐药大肠杆菌耐药表型的研究.采用常规方法和生化鉴定管对有过安普霉素用药史的鸡场分离的鸡源病原性大肠杆菌进行鉴定,并用试管二倍稀释法测定其最低抑菌浓度(MIC),筛选出鸡源安普霉素耐药大肠杆菌;采用药敏纸片法研究了这些耐药菌对安普霉素等14种抗菌药物的敏感性.共筛选出7株对安普霉素耐药的鸡源性大肠杆菌,这些耐药菌株全部对安普霉素、妥布霉素、奈啶酸、多西环素和阿莫西林耐药;大部分对庆大霉素、链霉素、卡那霉素、壮观霉素也呈现耐药.对新霉素的耐药较低,对阿米卡星高度敏感.部分交叉耐药现象的存在揭示对安普霉素等氨基糖苷类药物产生耐药性的菌株,其他抗生素也可能对它们失去疗效.  相似文献   
42.
将3个苏拉灭敏感的伊氏锥虫原种的克隆连续培养于改良Baltz无细胞培养系统中,通过逐步提高培养基中苏拉灭的含量,培育了3个抗苏拉灭的伊氏锥虫克隆─JGc1-160、JX-1c1-160和ZJc1-140。它们体外药敏试验的IC50依次为358.5、412.3和246.4μg/mL,是各自亲本克隆的1292.5、1874.1和1760.o倍;小鼠治疗试验的CD100,对免疫功能正常小鼠依次为80、120和30mg/kg,为各自亲本克隆的5.3、8.0和3.0倍,对免疫抑制小鼠为250、300和100mg/kg,分别是相应免疫正常小鼠的3.1、2.5和3.3倍。试验结果表明,抗锥虫药治疗剂量不足和宿主免疫功能不全是导致产生抗药虫株的重要因素,各自既可单独发挥作用,又可相互协同。本文报道了体外培育伊氏锥虫抗药虫株的方法,这一方法对研究锥虫抗药性具有重要作用。  相似文献   
43.
In 4 dairy farms located in Baotou area, clinical medicine investigation and drug resistance of pathogenic bacteria were conducted, and then curative effects of different treatment methods were analyzed.Epidemic materials were collected from diary cows infected by endometritis, from which pathogenic bacteria were isolated using conventional microbiology technologies.Broth microdilution method was used to detect the sensitivity and resistance of pathogenic bacteria to antibiotics commonly used in clinic.On this basis, the sensitive drugs were used to treat mastitis or endometritis, the clinical curative effect of traditional treatment group was compared with that of therapeutic test group.The result of traditional treatment group showed 13 antibiotics were used to treat 160 cases of endometritis, florfenicol was most effective, penicillin was most ineffective.The results of therapeutic test group showed that Staphylococcus aureus, Escherichia coli and Streptococcus were the main pathogenic bacteria of endometritis, which were sensitive to gentamicin and cefalotin, etc.The results of statistical analysis showed that the curative effect of therapeutic test group was extremely significantly different from that of traditional treatment group(P<0.01).Sensitive drugs screened by drug sensitive test could be used to target therapy, and obtain satisfied curative effect.Moreover, this approach could avoid antibiotic abuse.  相似文献   
44.
试验主要比较研究了6株乳酸杆菌对热(55℃和65℃)、酸(pH值1.0、2.0和3.0)、胆盐(0.1%和0.3%)、微量元素铜(0、10、50和250mg/L)和锌(0、120、600和3 000mg/L)的耐受性。结果表明:各菌株对这些因子的耐受性有不同程度的差异,如菌株LGG增殖速度、产酸性、耐酸性和耐温度55℃的能力都较其他菌株好,但该菌株在65℃下存活率极低;菌株ZJ614耐高温能力较强,在65℃处理20min后存活率能达到19.2%,但Cu2+(50 mg/L)对其增殖的不良影响较大;菌株ZJ621虽对酸的耐受性较强,但对胆盐的耐受性较差。经全面考察各菌株对各逆性因子的耐受性,初步推断,菌株ZJ610和ZJ617的综合耐逆性相对较强。  相似文献   
45.
抑制鹿源多药耐药结核菌中药的筛选   总被引:1,自引:0,他引:1  
为了筛选对鹿源多药耐药结核菌有效的中药耐药抑制剂,通过MABA法检测24种中药的水和乙醇提取物对多药耐药结核菌及标准菌H37Rv的体外抗菌效果,将筛选出的抗菌效果好的黄连醇提物和黄连水提物采用紫外分光光度法进行活性成分含量测定,同时以MABA法与标准品比较体外抗结核菌活性。结果显示,黄连、独活和侧柏叶有明显的抗结核活性,其中黄连水粗提物和乙醇粗提物效果最佳,测得它们活性成分即总生物碱含量分别为22.74%和35.23%,并显示二者与小檗碱的体外抗结核作用相同。  相似文献   
46.
抗旱耐热白三叶新品种选育初报   总被引:5,自引:0,他引:5  
鄂牧一号白三叶是以美国瑞加和路易斯安那两个白三叶品种为原始材料,经单株抗性造反份系比较鉴定,多系混合杂交,品种比较和区域试验选育而成,历时10年。该品种具有高产,抗旱,耐热和粗蛋白含量高等特性,是南方低山丘陵地区有推广应用前景的优良牧草和草坪植物。  相似文献   
47.
Reasons for performing study: Type 2 diabetes mellitus (T2DM) is diagnosed rarely in equine practice although it may be under‐recognised. A greater awareness of the condition and therapeutic considerations would be to the benefit of such cases presenting in practice. More investigation into the pharmacological management of these cases is needed. Objectives: Three cases of diabetes mellitus were investigated using a specific test for insulin sensitivity and pancreatic β cell function in order to define accurately and characterise the existence of T2DM in all 3 subjects. Methods: The insulin‐modified frequently sampled i.v. glucose tolerance test was performed in each case and the data so obtained were subject to minimal model analysis of insulin‐glucose dynamics. Cases were then monitored following treatment using a combination of dietary modification, metformin, glibenclamide and pergolide. Results: Marked insulin resistance was identified in each case and, furthermore, severe pancreatic β cell dysfunction was present therefore classifying each case as end stage T2DM. Treatment was nevertheless associated with restoration of normoglycaemia in all cases. Conclusions: T2DM in horses may be more common than generally considered. In some cases individuals may respond to therapy aimed at restoring insulin sensitivity and pancreatic function. Drugs used in other species for the treatment of T2DM have not yet been adequately tested in horses. Potential relevance: T2DM should be considered as an important differential diagnosis in mature to elderly horses and ponies suffering from weight loss, polydipsia and polyuria. Clinicians should be encouraged to offer treatment and management advice when such cases are encountered.  相似文献   
48.
BoLA基因同牛多种疾病和生产性状相联系,因此如何利用BoLA基因进行牛抗病育种是育种学家关心的问题,作者综述了BoLA基因同牛一些疾病的关系,并对BoLA基因进行牛抗病育种的现状及发展方向作了探讨。  相似文献   
49.
The tetracycline resistance gene, tet(B), has been described previously in Gram negative bacteria. In this study tet(B) was detected in plasmid extracts from 17/111 (15%) Streptococcus suis isolates from diseased pigs, representing the first report of this resistance gene in Gram positive bacteria.  相似文献   
50.
Reasons for performing study: Hyperinsulinaemia is detected in horses with insulin resistance (IR) and has previously been attributed to increased pancreatic insulin secretion. Connecting peptide (C‐peptide) can be measured to assess pancreatic function because it is secreted in equimolar amounts with insulin and does not undergo hepatic clearance. Hypothesis: A human double antibody radioimmunoassay (RIA) detects C‐peptide in equine serum and concentrations would reflect responses to different stimuli and conditions. Methods: A validation procedure was performed to assess the RIA. Six mature mares were selected and somatostatin administered i.v. as a primed continuous rate infusion, followed by 50 nmol human C‐peptide i.v. Insulin and C‐peptide concentrations were measured in horses (n = 6) undergoing an insulin‐modified frequently sampled i.v. glucose tolerance test, and in horses with insulin resistance (n = 10) or normal insulin sensitivity (n = 20). Results: A human RIA was validated for use with equine sera. Endogenous C‐peptide secretion was suppressed by somatostatin and median (range) clearance rate was 0.83 (0.15–1.61) ml/min/kg bwt. Mean ± s.d. C‐peptide‐to‐insulin ratio significantly (P = 0.004) decreased during the glucose tolerance test from 3.60 ± 1.95 prior to infusion to 1.03 ± 0.18 during the first 20 min following dextrose administration. Median C‐peptide and insulin concentrations were 1.5‐ and 9.5‐fold higher, respectively in horses with IR, compared with healthy horses. Conclusions: Endogenous C‐peptide secretion decreases in response to somatostatin and increases after dextrose infusion. Results suggest that relative insulin clearance decreases as pancreatic secretion increases in response to dextrose infusion. Hyperinsulinaemia in insulin resistant horses may be associated with both increased insulin secretion and decreased insulin clearance. Potential relevance: Both C‐peptide and insulin concentrations should be measured to assess pancreatic secretion and insulin clearance in horses.  相似文献   
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