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961.
Summary The aim of the present study was to investigate whether the resistance of beech foliage(Fagus sylvatica) against oxidative stress was affected by soil quality, nitrogen or CO2 fertilisation, or lachnid infestation(Phyllaphis fagi). For this purpose young beech trees were grown for four years in reconstructed calcareous or acidic forest soils in open
top chambers under ambient or elevated CO2 concentrations with two levels of nitrogen fertilisation. At harvest lachnid colonisation was observed, preferentially on
leaves from trees in calcareous soil and on leaves from trees fertilised with the high nitrogen level. General leaf characteristics
such as pigment concentrations, dry mass, and leaf mass ber area were not affected by the soil type, nitrogen fertilisation
or CO2 regime. Leaves colonised with lachnids displayed slightly increased leaf mass per area. When the stress resistance was challenged
by exposure to paraquat — a herbicide inducing oxidative stress — leaves from trees grown on calcareous soil maintained significantly
longer membrane integrity and, thus, were better protected against stress than leaves from trees on acidic soil. Other experimental
variables had negligible or no effects on the resistance against oxidative stress.
相似文献
962.
Idaho locust (Robinia pseudoacacia 'Idaho') is an exotic multi-purpose tree used in landscaping, soil and water conservation, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions.Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride (TTC) reductants in roots and soil water content (SWC). The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees. 相似文献
963.
翻译起始因子是一类翻译起始所必需的特异蛋白因子,前期研究表明柽柳翻译起始因子 (TheIF1A) 基因能对外界盐和干旱等非生物胁迫做出响应,且过表达 TheIF1A基因能提高酵母和烟草的抗旱耐盐能力。为进一步研究TheIF1A基因的抗逆机制,本研究通过酵母双杂交对柽柳翻译起始因子(TheIF1A) 基因的互作蛋白进行了筛选,共获得5个互作蛋白,分别为RNA聚合酶β II亚基 (RNA polymerase beta II subunit)、ATP合成酶CF1α亚基蛋白 (ATP synthase CF1 alpha subunit protein)、细胞色素b6/f 复合物亚基IV(cytochrome b6/f complex subunit IV)、核酮糖1,5-二磷酸羧化酶/加氧酶小亚基蛋白(ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit)和组蛋白乙酰转移酶(histone acetyltransferase)。利用实时荧光定量PCR对这5个蛋白基因及 TheIF1A 基因在盐和干旱胁迫处理下的表达模式进行分析,结果表明:这些蛋白基因在盐和干旱胁迫下的表达模式与 TheIF1A基因基本一致,表明TheIF1A可能通过与这些蛋白相互作用来参与逆境胁迫应答。为进一步研究TheIF1A 基因的抗逆机理奠定了基础,有利于完善林木抗逆机制的研究,并为通过基因工程手段提高林木抗逆性提供了候选基因。 相似文献
964.
Supercritical CO(2) fluid extraction (SFE-CO(2)) was used to extract the sapogenins after acid hydrolysis from Smilax china tubers. The influence of extraction variables, such as modifier, pressure, temperature and extraction time, were studied. SFE-CO(2) was found to produce higher yield than conventional solvent extraction. The highest yield (0.454%) of sapogenins, mostly containing diosgenin, was obtained using 35 MPa pressure, 65 degrees C and 95% EtOH as a modifier for 180 min, higher than that obtained with conventional extraction methods (0.385%). 相似文献
965.
966.
4 香豆酸 :CoA连接酶 (4CL)是木质素生物合成过程中重要的酶 .该文利用简并寡核苷酸PCR法结合cDNA末端快速扩增PCR法直接获得了紫穗槐 4CLAcDNA全长序列 ,避免了复杂的构建、筛选cDNA文库的过程 .先用简并PCR得到了 4CLA1片段 ,又据此片段设计反向嵌套引物 ,用RACE方法获得未知的 5′和 3′端序列 .所获得的全长 4CLAcDNA ,编码 5 40个氨基酸 .氨基酸序列同源性分析表明4CLA是典型的 4CL蛋白 ,含有预计的AMP binding位点、催化反应区和保守的Cys . 相似文献
967.
Bruno Vila Franck Torre Frdric Guibal Jean-Louis Martin 《Forest Ecology and Management》2003,180(1-3):413-424
Taking advantage of the introduction of the black-tailed deer to the Queen Charlotte Islands (British Columbia, Canada), we used dendrochronological analyses to understand the consequences of deer browsing on Sitka spruce growth. We compared shape, radial growth, height growth and age of young spruce in three sites. We identified two types of trees growing side by side: (1) stunted and heavily browsed spruce, smaller than the browsing limit and (2) escaped spruce that were taller than the browsing limit but still browsed in their lower part. The compact and heavily ramified shape in stunted spruce was the result of repeated and intense browsing. In escaped spruce this was also the case below the browsing limit (1.16 m±0.07 m), in sharp contrast with the normal shape that escaped spruce resumed above the browsing limit. We show that the release of browsing pressure, once the tree reaches the browsing limit, is characterised by an abrupt increase in radial growth. Before release, trees show a growth stagnation characterized by narrow rings (0.5 mm per year) and small annual height increments (<5 cm per year). After release, trees show a growth stabilisation characterised by wider rings (3 mm per year) and larger annual height increments (20 cm per year). We use this pattern to estimate frequency and age at release and their possible variation over time. Age differences between stunted and escaped spruce are highly significant and indicate that, despite of browsing, most if not all trees will ultimately reach the browsing limit and escape. Heavy deer pressure (30 deer per km2) delays spruce sapling recruitment by about 8 years. This delay varies in relation to site quality and seems to have increased over time, suggesting an increase in browsing pressure. 相似文献
968.
竹活性炭处理六价铬废水的试验研究 总被引:5,自引:1,他引:5
用竹子加工的下脚料制得的竹活性炭,对六价铬废水进行净化处理。结果表明,竹活性炭对六价铬废水处理效果良好,实验探讨了竹活性炭处理六价铬废水的一般规律及影响因素。 相似文献
969.
Dynamic properties of bamboo, Phyllostachys pubescens, with moisture content (MC) ranging from −130 to 130°C, were studied by dynamic mechanical analysis (DMA). The results showed
that the hygrothermal effect on dynamic mechanical properties was negative. The storage modulus decreases with increasing
temperature and MC, and glass transition temperature decreases with increasing MC. The glass transition temperature and tan
delta of bamboo were 30.5°C, 0.02 and 10.61°C, 0.04, when MC was 10% and 34%, respectively.
__________
Translated from Journal of Nanjing Forestry University (Natural Sciences Edition), 2006, 30(1): 65–68 [译自: 南京林业大学学报(自然科学版), 2006, 30(1): 65–68] 相似文献
970.
Sheng-Yang Wang Wan-Chi Lai Fang-Hua Chu Chien-Tsong Lin Shi-Yen Shen Shang-Tzen Chang 《Journal of Wood Science》2006,52(6):522-526
This is the first article to report the evaluation of a natural product used as an antisilverfish agent. Silverfish (Lepisma saccharina), primitive wingless insects, feed on a variety of materials, including paper, cotton, starch, and cereals. They can be a
problem in libraries and other places where books, documents, and papers are stored. In this pilot study, the essential oil
from leaves of Cryptomeria japonica was investigated to test its properties as a silverfish repellent and insecticide. The results from a repellency bioassay
show that the essential oil significantly repelled silverfish. The repellent activity was 80% at a dosage of 0.01 mg/cm3. When silverfish were exposed to a concentration of 0.16 mg/cm3 of essential oil, they were killed within 10h. The chemical composition of essential oil, the emissions from a test chamber,
and the residue left on filter papers previously soaked with the essential oil in a chamber were analyzed by gas chromatography-mass
spectrometry. The components of the essential oil were found to be: elemol (18.22%), 16-kaurene (11.63%), 3-carene (9.66%),
sabinene (9.37%), 4-terpineol (9.06%), β-eudesmol (5.70%), α-pinene (5.62%), and limonene (5.26%). Only some constituents of the essential oil compounds collected by solid-phase microextraction
were found to be emitted in the test chamber. The main constituents were: 3-carene (21.03%), p-cymene (10.95%), limonene (9.49%), β-myrcene (9.39%), γ-terpinene (9.10%), α-terpinene (8.57%), and 4-terpineol (7.97%). 相似文献