生姜癞皮病的发生危害及病原初步鉴定

生姜癞皮病在山东7月开始发病,8月中旬至9月中旬为盛发期。病株植株矮小、茎细、分枝少,叶色变淡,根茎表皮疣裂,根系腐烂,产量品质降低。病原物为南方根结线虫。砂质土壤、连作重茬、过量施钾肥均可加重该病的发生。     

Effect of atrial natriuretic peptide on acute lung injury induced by lipopolysaccharide

AIM: To investigate effect of atrial natriuretic peptide (ANP) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rat. METHODS: Mean arterial blood pressure (MAP) was recorded with model 6280 physiology intelligentialize grapher, nitric oxide (NO) and endothelin (ET) concentrations in plasma were measured after lipopolysaccharide (LPS) or following LPS ,ANP was injected into vein in rats. After experiment,lung water as well as pulmonary histopathological changes was measured and observed, respectively. RESULTS: Administration of LPS elicited a persistence decrease in MAP (8.1 kPa±2.6 kPa,at 4 h,P<0.01 vs control); NO and ET concentration in plasma was evident higher than that in control group, respectively (P<0.01); Wet-dry ratio of lung was higher than that in control group (5.15±0.43,at 4 h) (P<0.05); Alveolus detelectasis was observed and pulmonary mesenchyme was thicker than that in control group. No erythrocyte and leukocytes in alveolus,which show an interstitial pulmonary edema, was observed in LPS+ANP group, ANP maintained MAP at higher levels (13.35 kPa±2.93 kPa, at 4 h, P<0.05 vs LPS) after an transient decline when LPS was injected; NO and ET concentration of plasma had all significantly decrease, respectively (P<0.05 vs LPS, at 4 h); Wet-dry ratio of lung was lower than LPS group (4.57±0.35, P<0.05). Compared with control group the ratio was not evident difference (P>0.05); The histopathological of lung displayed markedly improved. CONCLUSION: ANP attenuates ALI induced by LPS in the rat. The effect of ANP may be via decreasing secretion of ET,NO and regulation arterial blood pressure.     

Factors affecting the development of disease symptoms in potatoes infected by <Emphasis Type="Italic">Tobacco rattle virus</Emphasis>

Infection of potato plants with Tobacco rattle virus by its nematode vector can have different outcomes, including the development of spraing symptoms in progeny tubers. A novel syndrome described here comprises a generalized mottle in leaves on all stems, together with virtually total failure to produce daughter tubers. The outcome of infection depends partly on the potato genotype, but field trials with 15 varieties showed that the incidence and severity of spraing symptoms at three sites differed. There was no evidence that this was due to differences in virulence among the virus isolates at the sites, but it was probably the result of environmental differences that influenced the numbers and activity of the vector nematodes. At the most severely affected site, spraing symptoms were found in all varieties tested, except Record, including several that were not affected at the other two sites. Taking both severity and incidence of symptoms into account, the ranking of varieties was similar at each site. The incidence of spraing symptoms was greater in larger than in smaller tubers, and increased with later harvest dates, but did not increase when early harvested tubers were stored. Tobacco rattle viruswas detected in the roots of many of the weeds growing at one of the sites and in the roots of a few plants of the subsequent barley crop.     

蜱传性疾病--莱姆病螺旋体研究进展

莱姆病是一种蜱传性人兽共患病,在我国北方各省广泛流行,其病原体为伯氏疏螺旋体。莱姆病可使皮肤、心脏、关节和神经系统等多种组织器官受损。本文综述了莱姆病螺旋体的形态、种下分类、生化特征、传播媒介和贮存宿主等方面的研究进展"     

鸡痘病毒通用高效表达载体的构建及其初步应用

利用分子克隆技术对本室构建的高效鸡痘病毒表达载体 p1 1 S进行改造 ,在其人工合成禽痘病毒 ( FPV)强启动子 Ps的下游引入含 7个单一酶切位点的多克隆位点 ( MCS) ,构建了便于外源基因插入的通用性更强的高效单表达载体 p N1 1 S。然后将 FPV早晚期启动子 PE/ L 及其启动的鹅源新城疫病毒 NDV ZJ1株的 F基因一并插入 p N1 1 S中 ,使PE/ L 与 Ps反向串联 ,从而构建出 1个含 NDV ZJ1株 F基因的重组 FPV高效双表达载体 p N1 1 SEF,其 MCS可以用于插入其他外源基因。在此基础上 ,将 NDV ZJ1株的 HN基因插入 p N1 1 SEF的 Ps启动子下游的 MCS中 ,构建了共表达 NDV ZJ1株 F和 HN基因的重组 FPV双表达载体 p N1 1 SEFHN。将 p H1 1 SEFHN质粒 DNA与 FPV2 82 E4株共转染 CEF,得到共表达 NDV ZJ1株和 HN基因的重组 FPV,间接免疫荧光实验初步证明外源基因得到了较好的表达。表明所构建的通用高效 FPV表达载体有利于高效基因工程活载体疫苗的研制 ,具有广阔的应用前景     

Magnetic resonance imaging of a brain abscess in a 10-month-old filly

The purpose of this paper was to correlate the magnetic resonance imaging (MRI) characteristics of a mature brain abscess in a horse with histopathologic alterations of brain tissue. Eight months after the onset of clinical signs, MRI of the brain of a 10-month-old filly was performed. A large space-occupying lesion in the right cerebral hemisphere was identified. This space-occupying lesion was delineated by a thick and well-defined capsule that was isointense to brain parenchyma on the T1-weighted images and with a markedly hypointense on the T2-weighted images. The identification of such a capsule is highly diagnostic of a mature brain abscess. The lesion seen on MR images was confirmed at necropsy where a large abscess of the right hemisphere was observed. Streptococcus zooepidemicus and Pseudomonas aeruginosa were isolated from the abscess. Based on histopathologic examination, the signal characteristics of the capsule on T1-weighted and T2-weighted images were found to be due to the presence of numerous hemosiderin-laden macrophages. These results are in agreement with previous studies on human patients. This report confirms the value of MRI in the diagnosis of equine brain diseases.     

Intramedullary Intervertebral Disk Extrusion in a Cat

Intervertebral disk extrusion is rarely reported in the cat. In this case, the clinical, radiologic, and magnetic resonance imaging findings of an acute intramedullary intervertebral disk extrusion in a 5-year-old domestic shorthaired cat are described. Radiographically, there was mineralized disk material within the vertebral canal. On magnetic resonance images, the disk material was found to be within the spinal cord. A linear disk trail extending from the disk space into the spinal cord may be specific for intramedullary disk extrusion.     

Viral diseases of the Ostrich (Struthio camelus var. domesticus)

The ostrich is an important animal in many livestock industries. A significant threat to this industry is losses from diseases. Newcastle disease is a notifiable, highly contagious viral infection of ostriches. Avian influenza may be transmitted from waterfowl, shorebirds and gulls to ostriches. Borna disease virus is a viral neurotropic infection spread mainly by rodents and felines. Crimean‐Congo hemorrhagic fever is a viral disease transmitted by Hyalomma ticks to humans. Avipoxvirus afflicts ostrich chicks and is transmitted by mosquitoes or by direct contact with a pox lesion. Maintenance of a healthy and profitable enterprise requires the implementation, with assistance from the local veterinary authority, of comprehensive, practical and effective methods of health management and preventative medicine.     

Association of hyponatremia and hyperglycemia with outcome in dogs with congestive heart failure

Objective: To evaluate plasma sodium and glucose concentrations in dogs with congestive heart failure (CHF) prior to treatment and evaluate the differences between survivors and non‐survivors. Design: Retrospective study. Animals: Fifty‐nine dogs with CHF prior to receiving cardiac medication. Interventions: None. Measurements and main results: The mean plasma sodium concentration in dogs with CHF was below the reference range (144–156 mmol/L) and significantly lower (P=0.009) in non‐survivors (141±6 mmol/L) compared with survivors (147±4 mmol/L). The mean plasma glucose concentration was above the reference range (76–117 mg/dL) and significantly higher (P=0.004) in non‐survivors (128±52 mg/dL) compared with survivors (100±13 mg/dL). Forty‐four percent of non‐survivors had concurrent low plasma sodium and high plasma glucose concentrations, whereas no survivors had both abnormalities (P<0.0001). Conclusions: Lower plasma sodium and higher plasma glucose are associated with a worse outcome in dogs with CHF.     

Characterization and PCR-based Typing of Xanthomonas campestris pv. vesicatoria from Peppers and Tomatoes in Serbia

During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante–iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante–iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed.