Isolation of peroxisomal enoyl-CoA hydratase in rainbow trout and immunochemical identification with the bifunctional enzyme

Peroxisomes are the sites for β-oxidation of long-chain fatty acids. The peroxisomal bifunctional enzyme (PBE) enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase catalyzes the second and third reactions of the β-oxidation system. Originally termed PPA-80 for peroxisome-proliferation associated 80,000 MW polypeptide, PBE levels are monitored to measure peroxisome proliferation in rodents and other species. The quantity of a 79,000 MW polypeptide in the light mitochondrial fraction of the liver, as analyzed by SDS-PAGE, increases when rainbow trout are exposed to peroxisome proliferating agents. This correlates with increases in acyl-CoA oxidase activity and peroxisome volume density. In the present study, peroxisomal enoyl-CoA hydratase was purified from trout liver and analyzed by immunoblotting with anti-PBE. A positive reaction with the 79,000 MW polypeptide band was observed providing strong evidence that this is the bifunctional enzyme.     

DMSO和Tween-80对黄芩悬浮细胞系的影响

采用植物细胞悬浮培养技术研究了有机溶剂二甲基亚砜（DMSO）和表面活性剂Tween-80对黄芩细胞悬浮系的影响。结果表明：0.5%，1%，5%（v/v）DMSO对黄芩悬浮细胞的生长没有促进作用。在黄芩细胞培养的后期（12d）添加浓度分别为0.5%、1%（v/v）的Tween-80对细胞的生长具有促进作用。5%（v/v）的DMSO和5%（v/v）的Tween-80对悬浮细胞内黄芩甙的积累都具有抑制作用，但能显著促进黄芩甙的胞外释放。     

Development and characterization of mouse monoclonal antibodies reactive with chicken CD80

This study was carried out to develop and characterize mouse monoclonal antibodies (mAbs) against chicken CD80 (chCD80). A recombinant plasmid containing a chCD80/horse IgG4 fusion gene was constructed and expressed in CHO cells to produce recombinant chCD80/IgG4 protein. Chicken CD80 was purified from the chCD80/IgG4 fusion protein following enterokinase digestion, and used to immunize BALB/c mice, resulting in 158 hybridomas that produced mAbs against chCD80. Three mAbs with high binding specificity for recombinant chCD80/IgG4-transfected CHO cells were identified by flow cytometry, and one of these (#112) was selected for further characterization. Immunoprecipitation of CD80/IgG4-CHO cell extract, or lipopolysaccharide (LPS)-treated monocytes identified 35.0 kDa proteins. Immunohistochemical analysis revealed chCD80-expressing cells exclusively in the bursal follicles at the outer portion of the cortex, and throughout the red pulp and the outer boundary of the white pulp in the spleen. By immunofluorescence microscopy, chCD80 was observed on intestinal dendritic cells. LPS treatment of bursa or spleen monocytes for 24 or 48 h increased chCD80 expression. Finally, addition of chCD80 mAb to Con A-stimulated spleen cells inhibited the expression of major histocompatibility complex class II antigens and IL-2-driven proliferation of lymphoblast cells. In summary, these chCD80 mAbs will serve as valuable immunological reagents for basic and applied poultry immunology research.     

Z80CPU构成实时测控专用微机优化设计

讨论用 Z80 CPU 构成最小系统的若干优化对策.通过软硬件的密切配合简化了接口设计,从而大量节约了芯片、降低了成本,并提高了抗干扰能力.该方法在小系统实时测控专用微机设计及智能仪器设计中具有较现实的意义.     

长白山原始红松阔叶林及其次生林细根分解动态和氮元素的变化

该文采用网袋分解法对长白山不同演替状态的原始红松阔叶林、白桦山杨成熟林和白桦山杨幼林中优势树种的细根分解动态及其氮元素的变化进行了研究.研究表明:在3块样地中,细根分解初期速率较快,然后趋于缓慢.在原始红松阔叶林中,水曲柳细根的分解最快,红松次之,而紫椴细根的分解最慢.在白桦山杨成熟林和幼林中,山杨的细根分解均比白桦细根快.细根的分解不仅受其本身化学性质的影响,而且也受到周围环境的影响.在原始红松阔叶林的演替过程中,细根分解过程中会释放或富集氮元素.温度较低和降雨较少的春秋季节,细根分解过程中主要表现为释放氮元素,而温度较高和降雨较多的夏季,则表现为富集氮元素.      

不同强度匹配的X80钢环焊接头力学性能及变形能力

环焊接头的强度匹配与韧性对管道服役安全至关重要。为了更深入掌握环焊缝的失效机理,采用力学性能测试、微观分析等方法,测试了两种不同强度匹配的高铌X80钢环焊接头的组织和性能,并借助数字图像相关法(Digital Image Correlation,DIC)研究了焊接接头在拉伸载荷下的应变行为。结果表明:低强匹配与高强匹配的环焊接头均具有较好的冲击韧性,二者的夏比冲击吸收能量平均值相当。在拉伸载荷下,应变集中最先出现在环焊缝的根焊及热影响区部位。随着拉伸载荷的增加,高强匹配环焊接头的应变集中逐渐转移至母材,管道承受轴向载荷及变形的能力大于低强匹配环焊接头;低强匹配环焊接头虽具有较好的韧性,但因其在焊缝及热影响区存在塑性应变累积效应,易发生断裂。(图9,表4,参22)     

抗病优质丰产甘蓝新品种秦甘80选育及其目标性状鉴定

秦甘80是利用具有抗病优质和配合力高的两个自交不亲和系B2523-3-2和FT6358-3-3配育成的杂交一代,为中熟甘蓝新品种,定植到叶球收获80d左右。植株综合经济性状优良,抗TuMV、Br、CMV 3种病害;中心柱6.2cm,紧实度0.53,帮叶比25.8％;叶质脆甜,富含营养物质,100g鲜重含粗蛋白1.125g,粗纤维0.514g,可溶性糖2.21g,VB_1208.97mg;平均产量75 980.3kg/hm~2,比对照京丰一号增产18.8％。     

酸乳中乳酸菌计数培养基的优选与改良

比较了5种乳酸菌计数培养基对乳酸菌的检测效果,其中TJA培养基乳酸菌检测率比LCM培养基、MRS培养基、改良CHALMERS部养基和LAB培养基分别高11.7%、44.8%、43.1%、和121.3%;在优选出的TJA培养基上分别添加不同质量分数的分散剂吐温-80进行改良,结果表明,改良TJA培养基以含0.1%吐温-80的检测效果最佳,对部分市售酸乳的检测与分析表明,酸乳成品中的乳酸菌数普遍大于1×108cfu/ML,个别品牌酸乳中的活性菌达到1×109cfu/ML.     

Poly(vinylidene fluoride)/poly(ethylene-co-vinyl acetate) (20/80) blend. I. Miscibility and crystallization behavior

The miscibility and crystallization behavior of the blends of poly(vinylidene fluoride) (PVDF) and ethylene/vinyl acetate(20/80) copolymer (EVAc80) have been studied using a differential scanning calorimeter and a polarizing microscope equipped with a heating stage. From the melting point depression, the values of interaction energy densityB were calculated to be −1.3004 (cal/cm³) and the Flory-Huggins interaction parameterχ ₁₂ was found to be −0.0818 at 445.6 K. With increasing concentration of EVAc80, the radial growth rate of spherulite was reduced drastically. The FT-IR analysis of samples quenched from the melt to various temperatures showed increasing content ofβ-phase with increasing amount of blended EVA80 along with lower quenching temperature.     

冷驯化和冷冻条件下不同抗寒性冬小麦品种三个COR基因表达差异的实时荧光定量分析

COR基因是含有CRT/DRE调控元件的一类冷响应基因,在植物抗寒耐寒过程中发挥着重要作用.为了分析不同抗寒性的冬小麦品种中COR基因表达的差异,明确在抗寒中起关键作用的COR基因,本实验以抗寒品种东农冬麦1号和不抗寒品种济麦22为材料,设定一系列低温处理,用实时荧光定量PCR(qRT-PCR)分析低温下Wrab17、Wcor80和Wcs120三个基因的表达差异.结果表明,在所有冷处理中,这三个基因在抗寒品种东农冬麦1号的相对表达量均比不抗寒品种济麦22高,上调表达时间更长,响应时间更短.处理组和对照组中也出现较大差异,在某些处理组中的相对表达量明显更高.其中冷驯化处理8～12 h和冷冻处理4h时Wrab17在东农冬麦1号中分别上调表达11.2、14.7和26倍,同时期同处理的济麦22中此数值分别为2.7、6.2和4.3倍;冷冻处理6h时Wcor80在东农冬麦1号中上调表达6.5倍,在济麦22中则出现小幅下调;冷驯化处理1h后Wcs120在东农冬麦1号中上调8.7倍,在济麦22中未发现上调表达.