鲜食制汁兼用型苹果优系选育初报

以4个苹果杂交组合后代为试材,对各杂种单株果实的外观品质、内在品质、加工指标以及浓缩汁的贮藏稳定性进行了评价。结果表明,杂种编号为97-060的1个苹果优系适于鲜食和制汁兼用。优系97-060的果实于9月底成熟,果实扁圆形,果面盖色红、全面着色,平均单果重156.8g;果肉松脆,出汁率为78.7%,风味甜酸;原汁可溶性固形物含量为13.5°Brix,原汁酸度为0.546%,浓汁酸度为2.981%。     

L929细胞无血清悬浮驯化机器在流产衣原体灭活疫苗中的应用研究

为了给规模化生产流产衣原体提供悬浮细胞培养方法,本研究通过逐步降血清悬浮驯化法使贴壁L929细胞逐步适应悬浮培养环境,最终获得了一株可无血清悬浮培养的L929细胞株;将流产衣原体接种于L929悬浮细胞上,研究不同的促感染试剂以提高收菌量,并选择出最佳的培养方法;制备流产衣原体灭活疫苗,以25 μL 、50 μL、100 μL的剂量免疫Balb/c小鼠,攻毒后检测其免疫保护效果。结果显示,当该L929悬浮细胞初始接种密度为5×105个/mL 时,培养72 h后细胞浓度可达到约7×106个/mL,细胞活力在95%以上;在细胞密度为6×106个/mL,接菌量为5.5×104 IFU/mL,加入脂质体2000的量为1.7 μL/mL、放线菌酮浓度为0.4 μg/mL时,菌量增殖倍数最大,48 h内约扩增了52倍。经流产衣原体悬浮细胞灭活疫苗免疫后,小鼠产生的抗体水平随免疫剂量的增加而提升;接种50 μL、100 μL疫苗组小鼠的脾淋巴细胞增殖水平、IFN-γ、IL-2的含量远高于接种25 μL疫苗和注射PBS组的小鼠;对免疫后的小鼠进行攻毒,接种疫苗组小鼠的脾脏、十二指肠、子宫中的流产衣原体基因组含量远低于未接种疫苗组小鼠。注射PBS组及接种25 μL疫苗组的小鼠子宫出现了不同程度的坏死及炎症,而其余两组小鼠的子宫无异常变化。结果表明,本试验成功驯化出１株L929悬浮培养细胞株,并且通过悬浮培养工艺制备的流产衣原体灭活疫苗免疫效果良好,接种50 μL、100 μL该灭活疫苗可以有效抑制流产衣原体的感染,该L929细胞全悬浮培养株的成功驯化为疫苗规模化生产提供了科学依据。     

Studies on green gram (Phaseolus aureus) protein concentrate and flour

Protein isolate from green gram (Phaseolus aureus) was prepared and the chemical composition was determined. It contained 64.04% protein, 1.8% total lipids, 27.64% total carbohydrates, 1.68% crude fibre and 4.84% ash. Iron, calcium, magnesium, copper, zinc, potassium and sodium were determined. The limiting amino acid in the protein isolate was lysine. In vitro digestibility pepsin followed the pancretion was the highest and the lowest was the digestion by pepsin alone. Water absorption, oil absorption, emulsion capacity and nitrogen solubility index (NSI) of the protein isolate were 2.26g/g, 1.24g/g, 31.4g/g and 6.8g/g, respectively. For comparison the same functional properties were determined for the flour of green gram. Replacing 5 and 10% of the wheat flour with green gram flour improved the mixing properties of dough and produced good acceptable bread. However, the addition of 15% green gram flour weakened the dough and lowered the quality of bread. Replacing 2.5, 5 and 7.5% of wheat flour with protein concentrate also weakened the mixing properties of the wheat dough and decreased the bread quality.     

30%阿维·灭幼脲悬浮剂配方的研制及田间药效试验

在室内生测验证具有明显增效的基础上,按照悬浮剂的制备工艺和技术指标控制试验的要求,进行了配方筛选试验,确定了30%阿维.灭幼脲悬浮剂的配方：29.7%灭幼脲,0.3%阿维菌素,1%NNO,4%农乳1600#,1%农乳500#, 1%凹凸棒土,1.2%拉开粉,0.1%黄原胶,5%乙二醇,0.3%硅酮类,水补至100%。配方产品的验证试验结果表明,30%阿维.灭幼脲悬浮剂的各项指标均能达到质量技术标准。田间药效试验结果表明,30%阿维.灭幼脲悬浮剂以有效成分180~220 g/hm2防治甘蓝小菜蛾,防效可达73.39%~80.19%吗,优于或等同于1.8%阿维菌素乳油（8.1 g/hm2)、20%灭幼脲悬浮剂（225 g/hm2）的防效,持效期达14 d。     

5%噻虫嗪水乳剂防治稻飞虱田间药效试验

采用5%噻虫嗪水乳剂防治稻飞虱田间药效试验。结果表明,5%噻虫嗪水乳剂有效成份用量为15.00、11.25、7.50 g/hm2对稻飞虱的防治效果均显著优于对照药剂25%噻虫嗪水分散粒剂有效成份用量7.5 g/hm2,药后第1天防效高达90.34%,药后第3天防效高达95.03%,药后第7天防效高达92.43%,且在试验作物上未见药害发生。     

哈尔滨市环境空气分析初报

根据哈尔滨市环境空气监测材料，对主要污染物质及其季节动态和空间分布进行了分析和研究，结果表明，哈尔滨市主要污染物质为二氧化硫、氮氧化物、悬浮颗粒物。其中，二氧化硫以一、四季度较高，空间分布上以道里区、道外区及太平区污染严重，南岗区污染最轻；氮氧化物在供暖期的一、四季度明显高于非供暖期的二、三季度，空间分布上以道里区、道外区污染相对严重；总悬浮颗粒物（TSP）以供暖期及初春秋末污染严重，空间分布上各行政区间差别较小。根据城市污染状况，笔者还对环境空气污染重点问题进行分析，并提出了相应的对策。     

Effects of dietary incorporation of potato protein concentrate and supplementation of methionine on growth and feed utilization of rainbow trout

Four diets (1, 2, 3 and 4) were formulated to contain different potato protein concentrate (PPC) levels (0, 22, 56, and 111 g kg⁻¹). Diet 5 contained 56 g kg⁻¹ PPC and 17 g kg⁻¹ methionine. A growth trial was conducted to investigate the effect on growth and feed utilization of incorporation of PPC and supplementation of methionine in the diet of rainbow trout. When the proportion of PPC exceeded 56 g kg⁻¹ the growth of fish decreased while both growth and feed utilization decreased when the dietary PPC was 111 g kg⁻¹. Protein productive value and condition factor of the fish decreased and mortality increased with the increase in the proportion of dietary PPC.     

三种热雾剂对橡胶树炭疽病大田防治试验

田间试验比较10%百菌清、20%氟硅唑·咪鲜胺、16%百菌清·咪鲜胺·三唑酮三种热雾剂对大田橡胶树炭疽病的防治试验结果,其中前两种处理防效(相对防效68.14%和66.31%)显著优于16%百菌清·咪鲜胺·三唑酮和对照处理。     

香蕉假茎外植体愈伤组织诱导的初步研究

[目的]研究香蕉假茎外植体愈伤组织的最佳培养基.[方法]以香蕉组培无菌苗假茎为外植体,接种在含不同浓度配比激素的固体培养基中进行诱导培养,再将培养获得的胚性愈伤组织接种到液体培养基中进行悬浮培养.[结果]使用低浓度噻苯隆（TDZ）诱导出胚性愈伤组织的效果最好,最佳培养基为T2：MS＋0.02 mg/L TDZ ＋7 mg/L2,4-D.[结论]悬浮培养使用ZT2能较早建立ECS,且继代效果较好.     

台湾金线莲细胞悬浮培养及其培养条件优化的研究

[目的]研究台湾金线莲细胞悬浮培养B5培养基的配方.[方法]以新鲜叶片为材料,经过初代培养、固体培养和继代培养,建立悬浮细胞培养体系,正交设计试验优化培养基配方.[结果]B5培养基中,影响悬浮培养因素的大小依次为大量元素浓度、6-BA、2,4-D、蔗糖、IAA.[结论]选用2％蔗糖、0.5 mg/L 6-BA、0.2 mg/L IAA、0.6 mg/L 2,4-D、1/2浓度大量元素的B5培养基培养悬浮细胞,15 d后吸光度达4.425.