息烽县住宅黄胸鼠种群数量监测

黄胸鼠种群数量发生动态分析结果可以为该鼠的预测预报及防治提供依据。本文分析了1986-2015年间贵州省息烽县黄胸鼠种群数量调查数据,总结了其季节性及年际种群数量动态。结果发现,在息烽县,黄胸鼠主要栖息于住宅区,作物区仅有零星分布。期间共捕获黄胸鼠938只,总捕获率为1.07%,住宅区黄胸鼠占总捕获鼠数的15.20%,不同年度间其群落占比为1.14%~32.49%,变化差异极显著。从季节性捕获率看,种群数量夏季(6~8月)最高,总平均捕获率为1.32%,其中7月捕获率为1.55%,是全年最高点。冬季(12月至翌年2月)最低,总平均捕获率为0.66%,其中1月数量最低,平均捕获率为0.49%。     

入侵种黄胸鼠(Rattus flavipectus)在山西省的分布特征及发展趋势

自1991年山西省农业科学院植物保护研究所科研人员发现黄胸鼠(Rattus flavipectus Milne Ed-wards)入侵山西省临汾市以来,黄胸鼠的分布区域现已扩展到运城市、长治市、晋中市、太原市等地区。对黄胸鼠入侵山西省后的分布特征、栖息环境、发展趋势等方面进行了研究,发现黄胸鼠极有可能进一步成为山西省的主要家栖鼠,对其有必要进行深入研究。     

新疆南部农区鼠害调查与成因分析

南疆农区鼠害严重,但基础资料不足。为摸清当地鼠害发生特点,提供有效治理依据,本研究于2018年-2019年在南疆5地州39乡64村开展实地与问卷调查,并采用夹捕法调查害鼠种群构成。结果表明,南疆5地州农村普遍遭受鼠害影响,喀什与和田地区农田受害比例达89.4%,问卷结果表明农户和农田鼠害发生率均大于75%,且西部地区重于东部,约一半受访农户估计每年因鼠害损失超过500元。褐家鼠Rattus norvegicus是农户主要害鼠,占比超过70%;农田则以小家鼠Mus musculus和乌拉尔姬鼠Apodemus uralensis为主。这些结果表明,南疆农区鼠害处于重发态势,褐家鼠是主要鼠害来源,并有外来入侵风险。下一步应聚焦于监测主要害鼠种群动态规律,阐明褐家鼠来源和侵入机制,并探索建立适于当地的害鼠防治模式。     

Evaluation of truncated LipL32 expressed by Escherichia coli and Pichia pastoris for serodiagnosis of Leptospira infection in rodents

The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then, truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared between two recombinant expression hosts Escherichia coli and Pichia pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris (tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E. coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results from flaB gene PCR of kidney samples and WB with whole Leptospira cell. Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32 expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of background reaction.     

Brodifacoum is effective against Norway rats (Rattus norvegicus) in a tyrosine139cysteine focus of anticoagulant resistance in Westphalia,Germany

BACKGROUND: The tyrosine to cysteine amino acid substitution at location 139 of the vkorc1 protein (i.e. tyrosine139cysteine or Y139C) is the most widespread anticoagulant resistance mutation in Norway rats (Rattus norvegicus Berk.) in Europe. Field trials were conducted to determine the incidence of the Y139C mutation at two rat‐infested farms in Westphalia, Germany, and to estimate the practical efficacy against them of applications, using a pulsed baiting treatment regime, of a proprietary bait (Klerat^?) containing 0.005% brodifacoum. RESULTS: DNA analysis for the Y139C mutation showed that resistant rats were prevalent at the two farms, with an incidence of 80.0 and 78.6% respectively. Applications of brodifacoum bait achieved results of 99.2 and 100.0% control at the two farms, when measured by census baiting, although the treatment was somewhat prolonged at one site, possibly owing to the abundance of attractive alternative food. CONCLUSION: The study showed that 0.005% brodifacoum bait is fully effective against Norway rats possessing the Y139C mutation at the Münsterland focus and is likely to be so elsewhere in Europe where this mutation is found. The pulsed baiting regime reduced to relatively low levels the quantity of bait required to control these two substantial resistant Norway rat infestations. Previous studies had shown much larger quantities of bromadiolone and difenacoum baits used in largely ineffective treatments against Y139C resistant rats in the Münsterland. These results should be considered when making decisions about the use of anticoagulants against resistant Norway rats and their potential environmental impacts. Copyright © 2012 Society of Chemical Industry     

晋西南农林生态区社鼠种群数量的季节消长研究

1993年5～11月份、1999年4～10月份和2006年的5～11月份,在地处晋西南的隰县阳头升乡竹干村和后堰村,以铗捕率或笼捕率的变化作为衡量种群数量的标准,开展了对社鼠(Rattus confucianus sacer)种群数量季节消长的研究。结果表明,社鼠种群数量存在着明显的季节变化,主要是繁殖和死亡两大因素相互作用的结果。种群每年在9月份出现一次数量高峰,由5、6、7、8月份的繁殖行为形成。10月份以后,由于气候和食物等条件恶化,致使部分鼠体死亡,特别是上一年越冬鼠的大量死亡,使社鼠种群数量随之下降。     

增效溴敌隆对几种害鼠的毒杀作用

本文涉及一种抗凝血增效灭鼠剂的研制和应用.该灭鼠剂是以溴敌隆为主剂、以双氯芬酸钠、丙三醇等为辅剂,可制成液剂和粉剂.经生物测定表明,对大白鼠、小白鼠、褐家鼠、大仓鼠和布氏田鼠均有很好的毒效,毒杀率基本达到100％,且适口性良好.现场试验对褐家鼠防效达96％以上.该药中溴敌隆含量为0.002％时,防效与0.005％溴敌隆相当起到了增效的作用,降低了成本,减少了环境污染,保证了人畜安全.     

Habitat preferences and distribution of the brown rat (Rattus norvegicus Berk.) in the city of Salzburg (Austria): implications for an urban rat management

The population of Rattus norvegicus was assessed using live traps and hair sampling tubes in 71 discrete patches distributed in the urban area of the city of Salzburg, Austria. R. norvegicus occurred in 35% of the examined habitats and reached a maximum density of 113.12 individuals/km along water banks. The occurrence of rats was strongly influenced by vegetation, habitat modification and man-made impact in the area. The results of this study could be used to support the development of a new, more scientifically based rat management plan in cooperation with the city of Salzburg.