提高威宁县马铃薯脱毒种薯质量的关键技术

从选种、播种密度、播种时间、去杂、蚜虫防治、种薯质量检测和适时收获等方面阐述了提高威宁县马铃薯种薯质量的关键技术。     

野生水禽鸭瘟病毒的分离与鉴定

取急性死亡的某动物园野生水禽进行病毒分离,通过鸡(鸭)胚接种、动物回归试验、病毒主要理化特性测定、免疫预防试验等证实分离株为鸭瘟病毒,揭示野生水禽完全有自然感染鸭瘟的可能性,家鸭与野生水禽在相互间传播鸭瘟病毒上具有双向性.     

Use of Chaetomium globosum for biocontrol of potato late blight disease

The efficacy of Chaetomium globosum as a biocontrol agent against the late blight pathogen Phytophthora infestans was evaluated in potato plants. Among eight Chaetomium isolates evaluated C. globosum isolate Cg-6 showed greater inhibition to mycelial growth of P. infestans in vitro. TLC studies showed that isolate Cg-6 produced an antibiotic called ‘Chaetomin’. Isolate Cg-6 showed greater exo- and endo-glucanase enzyme activity when compared to other isolates. PCR amplification of the ITS region and sequencing of the PCR product confirmed that isolate Cg-6 belongs to the C. globosum group. C. globosum Cg-6 was formulated as a liquid and applied as a tuber, soil and foliar treatment either individually or in combination against Phytophthora infection in potato plants. Among different treatments, combined application of C. globosum as a tuber treatment @ 1 ml/kg of tubers, as a soil application @ 1 ml/kg of Farm Yard Manure (FYM) and foliar spray @ 0.7% resulted in significantly less late blight infection (72%) compared to untreated control (100%) under field conditions. The application of C. globosum resulted in greater tuber yield by reducing late blight infection in two field trials when compared to untreated controls. The study clearly demonstrated the potential use of C. globosum as a biocontrol agent in the management of late blight disease in potato plants.     

表达传染性法氏囊病病毒VP2基因的重组鸡痘病毒的构建及其免疫保护性研究

以脂质体转染技术构建了表达鸡传染性法氏囊病病毒（ＩＢＤＶ）ＶＰ２基因的重组鸡痘病毒ＦＰＶ－ＶＰ２，该病毒在鸡胚成纤维细胞及鸡体内均能稳定产生子代病毒，经翅皮下５×１０５ＰＦＵ／羽免疫１日龄ＳＰＦ鸡，免疫后４周以１００ＬＤ５０／羽ＩＢＤＶ超强毒株Ｇ株攻毒，获得了５／６的保护，但不能有效预防临床发病及法氏囊受损萎缩。实验结果证明了ＶＰ２是ＩＢＤＶ的宿主保护性抗原，提示Ｔ细胞介导的免疫可能在ＩＢＤＶ的免疫中起着较为重要的作用。本研究为ＩＢＤＶ重组病毒疫苗研制进行了有益探索。     

Improved detection of citrus psorosis virus using polyclonal and monoclonal antibodies

Citrus psorosis is a serious and widespread disease associated with citrus psorosis virus (CPsV), a novel filamentous negative-stranded virus in the genus Ophiovirus . Laborious and costly indexing on test plants has been the only routine diagnostic method available, but recently an antiserum usable in double antibody sandwich (DAS) ELISA has been prepared. Here, major improvements to the DAS-ELISA protocol, a new purification method, and production of two monoclonal antibodies (mabs) to CPsV, an IgG and an IgM are reported. A highly sensitive triple antibody sandwich (TAS) ELISA making use of the mabs is described. In glasshouse citrus the homologous virus was still detectable at a tissue dilution of 1/6250 in DAS and at 1/31250 in TAS-ELISA. Both the DAS and IgG mab-TAS formats detected all CPsV isolates so far tested (from Argentina, Italy, Lebanon, Spain and the USA). A few isolates were not detected by the IgM mab.     

马铃薯包衣技术研究初报

不同药剂包衣对马铃薯影响不同。用58％甲霜灵锰锌可湿性粉剂、20％病毒A可湿性粉剂包衣可防治马铃薯晚疫病和病毒病,显著提高出苗率和产量。     

Associations of Gonadotropin-Releasing Hormone Receptor (GnRHR) and Neuropeptide Y (NPY) Genes'''' Polymorphisms with Egg-Laying Traits in Wenchang Chicken

Single nucleotide polymorphisms (SNP) of chicken gonadotropin-releasing hormone receptor (GnRHR) and neuropeptide Y (NPY) were selected to identify the genotypes of Wenchang (Chinese indigenous breed) chicken with restricton fragment length polymorphisms. The associations of the SNPs with the total egg production (NE), average days of continual laying (ADCL), and number of double-yolked eggs (DYE) traits were analyzed. The frequency of restriction enzyme A/a alleles in the population was for GnRHR 0.69 (Bpu1102 Ⅰ A) and 0.31 (Bpu1102 Ⅰ a) and for NPY 0.46 (Dra Ⅰ B) and 0.54 (Dra Ⅰ b). Trait data from a total of 120 hens, which were purebred introduced from Hainan Province, China from one generation were recorded. Two significant effects of genes' marker were found for GnRHR and number of eggs (dominant; t= 2.67, df= 116) and NPY and number of eggs (additive; t= 1.97, df= 116). The current research supports the effects of GnRHR and NPY genes on egg-laying traits of chickens.     

禽网状内皮组织增生病病毒感染和鸡群的免疫抑制

近几年来，我国鸡群中一些免疫抑制性综合症候，越来越常见。不久前，我们分别在江苏和山东的二个种鸡场遇到了典型的例。虽然二个鸡群对网状的内皮增生病病毒（ＲＥＶ）和鸡贫血病毒都有较高的感染率。但在对鸡群的系统病史、病理剖解变化实验室检查结果做综合分析判断的基础上，确定这二个鸡群的免疫抑制主要是由于整个鸡群在早期感染了网状内皮增生病病毒所致。本文还讨论了造成这种早期感染的可能原因。     

Prokaryotic Expression and Bioinformatics Analysis of Nonstructure Protein 3C from Porcine Encephalomyocarditis Virus

To obtain the 3C protein of encephalomypcarditis virus (EMCV) and predict its structure,3C gene was amplified by RT-PCR with the RNA as template from HB10 strain. And the target gene inserted into pMD18-T vector. The plasmids were sequenced after verification by PCR and double enzyme digestion. The target gene was cleaved from the correct plasmid,and inserted into the pET32a vector. The recombinant pET32a-EMCV-3C was transformed into TranSetta (DE3) and then induced by IPTG. Then the size was identified by SDS-PAGE and the antigenicity was analyzed by Western blotting. According to the sequence results,the length of 3C gene was 615 bp,which encoded 205 amino acids. The results of SDS-PAGE showed that His-3C protein mainly existed in the form of inclusion body with the molecular of 40 ku. The Western blotting results verified that purified His-3C could react with rabbit anti-EMCV serum prepared with EMCV. Bioinformatics analysis indicated that 3C protein was non-secretory,and had multiple phosphorylation sites,but it had no transmembrane region. Thus,the 3C protein mainly involved in protein hydrolysis process.3C protease,as the only protease in the EMCV genome,played an indispensable role in viral replication. In this study,we successfully constructed the prokaryotic expression vector of 3C protein and predicted its structure,which provided a basis for further study of the role of 3C protein and catalytic mechanism.