藿香植物组织培养技术研究

选用藿香健壮枝条和叶片作为试验材料,进行组培技术的研究.结果表明:藿香取材季节以夏季较好,适宜的外植体种类为茎段.诱导愈伤组织形成的激素组合为6-BA 1.0mg/L NAA 0.3 mg/L 2,4-D 0.3 mg/L,具有愈伤组织生长速度快并易于分化;不定芽分化激素组合为6-BA 0.5 mg/L NAA 0.1 mg/L,继代培养组合6-BA 0.1 mg/L NAA 0.5 mg/L B90.3mg/L,增殖在8倍以上;生根培养组合1/2MS NAA0.6 mg/L,一般20 d左右生根,平均根量4～5条,生根率达92%.     

Kinetics of Interferon-Gamma Production and its Comparison with Anti-Listeriolysin O Detection in Experimental Bovine Listeriosis

The kinetics of the production of interferon gamma (IFN-ORDER="0">) in whole blood culture and its comparison with anti-listeriolysin O (ALLO) detection by ELISA were studied during oral infection of calves with Listeria monocytogenes. Culture filtrate antigen (CFA), listeriolysin O (LLO), and sonicated antigen (SA) were used to prime the peripheral blood mononuclear cells (PBMCs) and the plasma from orally infected calves. IFN-ORDER="0"> and ALLO appeared as early as day 7 of an oral infection. IFN-ORDER="0"> was detected earlier with LLO than with SA. The Max50 interleukin (IL-2) activity and IFN-ORDER="0"> estimated in the culture supernatant from PBMCs primed in vitro with different antigens of L. monocytogenes revealed high induction of IL-2 and IFN-ORDER="0"> by CFA, LLO and live antigen. IFN-ORDER="0"> assay and ALLO detection were used for testing cases of repeat breeding in dairy cattle. It appeared that detection of IFN-ORDER="0"> employing LLO can be used to diagnose listerial infections.     

Ni/Al2O3-MxOy催化剂催化松香氢化反应研究

研究了自制非贵金属纳米催化剂Ni/Al2O3-MxOy催化松香的氢化反应。结果表明,松香氢化的最适工艺条件为:反应温度210℃、反应压力4.5 MPa、反应时间2 h、催化剂用量2.5%、搅拌转速500 r/min,在此条件下枞酸转化率可达98%以上,催化剂可重复使用4次。     

泰安市污水处理厂处理城市污水的研究

本文详细阐述了AB工艺的原理及A2O工艺的特点。并就测得的数据分析了CODcr与BOD5之间显著的线性关系;对年间、季度间的CODcr去除率进行方差分析,结果表明CODcr年间去除率存在一定差异,季度间去除率稳定;通过比较TP与TN的去除率,分析了具有脱氮除磷功能的A2O工艺在实际运行中存在的问题。     

Comparison of sampling and isolation procedures for recovery of Yersinia enterocolitica serotype O:3 from the oral cavity of slaughter pigs

Yersinia enterocolitica serotype 0:3/biotype 4 was isolated from the oral cavity of altogether 32 (68.1 %) of 47 freshly eviscerated slaughter pigs. Most efficient recovery was achieved by cultivation of tissue samples from both tongue and tonsils of the same individual. The isolation rate so obtained was significantly higher than that obtained by separate examination of either tonsil swabs or tongue swabs. However, the isolation frequency achieved by combined swabbing of the 2 sites was not significantly different. In general, tonsils were more productive for the recovery of 0:3 strains than were tongues, and tissue samples yielded higher isolation rates than did swabs. Three-week cold enrichment in a low selective medium proved essential for optimal recovery. However, the highest number of isolates was obtained using a combination of methods, including direct plating and selective enrichment in a modified Rappaport broth in addition to cold enrichment.     

内蒙古草原N2O和CH4排放通量及其季节变化特征研究

１９９８年５－１２月，作者在内蒙古半干旱原用静态箱法进行原位Ｎ２Ｏ和ＣＨ４排放通量观测，分析了Ｎ２Ｏ、ＣＨ４排放量及其季节变化和相关过程，定时估计草原Ｎ２Ｏ和ＣＨ４收支在人类活动下的变化。重点讨论了内蒙古古草原型群落羊草，大针茅和退化草原Ｎ２Ｏ和ＣＨ４的排放通量及其季节变化特征。     

铅丹药学研究进展

在广泛文献检索的基础上，对铅丹的临床应用、毒性和注意事项等进行了综述，为全民养生保健提供科学参考。     

焊接机器人三维防碰撞力传感器的设计

设计了一种三维防碰撞力传感器,其可利用一个输入实现x、y、z三个方向的碰撞力测试,用在6-D.O.F串联焊接机器人末端时,既可以实现对机器人本身的保护,也可以安装在其他设备的末端执行器上实现对设备的保护.     

一种制备(3R,4R)-4,7,7-三甲基-6-氧杂二环[3.2.1]辛烷-3,4-二醇的新方法

在催化剂过氧磷钨酸十二烷基吡啶盐(Cat-PW4)的作用下,α-蒎烯与H2O2反应生成主要产物(3R,4R)-4,7,7-三甲基-6-氧杂二环[3.2.1]辛烷-3,4-二醇。不同反应条件对反应转化率和选择性的实验结果表明,最佳反应条件为:12.8 mmolα-蒎烯、5 m L溶剂三氯甲烷、0.2 g催化剂、3.3 m L 30%H2O2,反应温度40℃,反应时间3 h,α-蒎烯转化率和产物的选择性分别为94.7%和39.8%。反应结束后,该产物存在于水相和有机相中,通过萃取和重结晶分离提纯,得率11%,纯度达到98%;其分子结构通过红外光谱、紫外光谱、1H核磁共振谱、13C核磁共振谱、低分辨率质谱及高分辨率质谱确证。     

某牛场粪源大肠杆菌0157∶H7的分离鉴定

为了解重庆市某牛场牛群大肠杆菌O157∶H7的感染情况,我们以牛场犊牛为研究对象,采集其新鲜粪便,经LB培养基增菌培养、免疫磁珠富集后,在山梨醇麦康凯琼脂上划线培养,最后PCR扩增eaeA基因目的片段,以此分离鉴定粪源大肠杆菌O157∶H7。结果显示：在所采集的39份犊牛粪便样品中,分离得到一株符合大肠杆菌O157∶H7生长特点且含有eaeA基因的菌株。