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41.
构建一种基于荧光量子点免疫探针的小麦面粉中赭曲霉毒素A(OTA)的检测方法。以EDC为活化剂将CdSe/ZnS半导体荧光量子点与OTA单克隆抗体偶联,制备OTA检测探针并评价其检测性能。结果表明:制备的检测探针在3d保存期内具有良好的稳定性。当OTA质量浓度为0.1~0.9μg/L时,检测探针的荧光强度与OTA质量浓度呈线性关系,其相关系数为0.996 3,检出限为0.017 7μg/L。与国标法对照的结果说明,此方法具有可行性。小麦面粉中的加标回收试验结果显示,OTA的回收率为96.0%~102.7%,相对标准偏差不超过4.1%,说明准确性良好。可见,OTA的CdSe/ZnS荧光量子点免疫探针检测方法操作简单、准确度高、结果稳定,有望应用于小麦面粉中OTA的检测。 相似文献
42.
近年来,伴随我国畜牧业蓬勃发展的同时,为了更大限度的提高母畜的受胎率和充分利用优秀种畜的基因,人工授精技术得到了不断完善和发展,并在全国各地广泛推广和应用。本文结合实践经验总结出的一些技术措施,阐述了对早胜牛这一地方优良种质资源进行保种和开发利用的重要意义,同时指出人工授精技术和低温冷冻保存精液技术在早胜牛上的实践,对于进一步开发利用培育优质高产肉牛新品种,促进肉牛产业高质量发展具有非常重要的现实意义。通过早胜牛种公牛采精的全过程以及细管冻精制作要点的详细讲解,低温冷冻保存精液技术,可更经济、可靠地实现家畜品种资源的保护,让优良基因长时间保存使得后续研究需要得到保障。 相似文献
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Gut-lung axis injury is a common finding in patients with respiratory diseases as well as in animal model of influenza virus infection. Influenza virus damages the intestinal microecology while affecting the lungs. Rifaximin, a non-absorbable derivative of rifamycin, is an effective antibiotic that acts by inhibiting bacterial RNA synthesis. This study aimed to determine whether rifaximin-perturbation of the intestinal microbiome leads to protective effects against influenza infection, via the gut-lung axis. Our results showed that influenza virus infection caused inflammation of and damage to the lungs. The expression of tight junction proteins in the lung and colon of H1N1 infected mice decreased significantly, attesting that the barrier structure of the lung and colon was damaged. Due to this perturbation in the gut-lung axis, the intestinal microbiota became imbalanced as Escherichia coli bacteria replicated opportunistically, causing intestinal injury. When influenza infection was treated with rifamixin, qPCR results from the gut showed significant increases in Lactobacillus and Bifidobacterium populations, while Escherichia coli populations markedly decreased. Furthermore, pathology sections and western blotting results illustrated that rifaximin treatment strengthened the physical barriers of the lung-gut axis through increased expression of tight junction protein in the colon and lungs. These results indicated that rifaximin ameliorated lung and intestine injury induced by influenza virus infection. The mechanisms identified were the regulation of gut flora balance and intestinal and lung permeability, which might be related to the regulation of the gut-lung axis. Rifaximin might be useful as a co-treatment drug for the prevention of influenza virus infection. 相似文献
46.
Two 60‐day experiments were conducted sequentially to determine (i) lysine requirement of juvenile bluegill, Lepomis macrochirus based on the dose–response method, (ii) requirements for other essential amino acids (EAAs) using whole‐body amino acid profile and (iii) whether differences in growth rates of group‐housed versus individually‐housed bluegills lead to different lysine requirement levels because of the presence and absence, respectively, of social hierarchies. Seven, semi‐purified, experimental diets (isonitrogenous, isocaloric) were prepared to contain graded levels of digestible lysine (10–31 g kg−1). Experiment‐1 involved group‐housed bluegills (approximately 27 g, n = 10 fish/chamber, 4 chambers/diet) whereas experiment‐2 involved individually‐housed bluegills (approximately 30 g, n = 1 fish/chamber, 14 chambers/diet). Fish were fed twice daily to apparent satiation. Bluegill growth responses in both experiments generally improved (P < 0.05, anova ) with increasing dietary lysine levels from 10 to 16 g kg−1, and then levelled off with further increase in lysine level (P > 0.05). Optimal dietary lysine level (digestible basis) was estimated to be 15 g kg−1 based on broken‐line regression analyses of relative growth rate and feed conversion ratio with no differences being observed between the two rearing methods. Determined dietary requirement levels for other EAAs ranged from 2.4 g kg−1 (tryptophan) to 15.3 g kg−1 (leucine). 相似文献
47.
《International Journal of Fruit Science》2013,13(1):91-105
Abstract This paper focuses on the cropping systems in central European countries such as Belgium, The Netherlands, UK, Ireland, Germany, Switzerland and the northern part of France and Italy. During the last decade there has been a consistent increase in programmed “out-of-season” strawberry production in several central European countries. This is being achieved in part, by using cultivars with different cropping seasons, but mainly by sequential planting of cold-stored plants and by growing strawberries in greenhouses and plastic tunnels. These methods have made it possible to extend the strawberry production for 11 months, from the end of February until mid January in central European countries with moderate climates. 相似文献
48.
鸭甲型肝炎(duck hepatitis A,DHA)是危害养鸭业的主要疾病之一,是一种雏鸭的急性、高度致死性传染病。本研究旨在研制一种对我国流行的鸭甲型肝炎血清1型(DHAV-1)和血清3型(DHAV-3)病毒具有交叉中和作用的单克隆抗体(monoclonal antibody,McAb)。人工合成12条DHAV-1和DHAV-3的VP1蛋白共有的抗原表位肽,分别与钥孔血蓝蛋白(keyhole limpet hemocyanin,KLH)载体偶联作为免疫抗原,免疫BALB/c小鼠制备McAb。通过检测McAb与DHAV-1和DHAV-3的交叉反应性,测定McAb对病毒增殖的抑制效率、中和效价以及攻毒保护率等来筛选McAb。本研究共获得了12株稳定分泌McAb的杂交瘤细胞株,其中6株(C1、C4、C7、C12、C13、C16)分泌的McAb同时与DHAV-1和DHAV-3发生特异性交叉反应; C1、C4、C7、C16可以抑制DHAV-1和DHAV-3在鸭胚中的增殖,抑制率在75.34%~100.00%不等;对DHAV-1和DHAV-3的中和效价:C1(1:3&1:5)、C4(1:3&1:3)、C7(1:10&1:11)和C16(1:9&1:9);C7和C16对DHAV-1和DHAV-3攻毒雏鸭的保护率较高,分别为“70%、80%”和“100%、60%”。本研究成功研制出对DHAV-1和DHAV-3具有交叉中和活性的McAb 4株,其中2株对病毒的感染具有良好的预防效果,为DHA的防控提供了新材料和新思路。 相似文献
49.
Genetic bleeding disorders can have a profound impact on a horse's health and athletic career. As such, it is important to understand the mechanisms of these diseases and how they are diagnosed. These diseases include haemophilia A, von Willebrand disease, prekallikrein deficiency, Glanzmann's Thrombasthenia and Atypical Equine Thrombasthenia. Exercise-induced pulmonary haemorrhage also has a proposed genetic component. Genetic mutations have been identified for haemophilia A and Glanzmann's Thrombasthenia in the horse. Mutations are known for von Willebrand disease and prekallikrein deficiency in other species. In the absence of genetic tests, bleeding disorders are typically diagnosed by measuring platelet function, von Willebrand factor, and other coagulation protein levels and activities. For autosomal recessive diseases, genetic testing can prevent the breeding of two carriers. 相似文献
50.
Malgorzata Grzesiak Magdalena Socha Anna Hrabia 《Reproduction in domestic animals》2021,56(1):193-196
This study aimed to examine 25OHD3 concentration in the fluid of follicular and follicular lutein cysts of sows in comparison with preovulatory follicles as well as immunolocalize vitamin D metabolic enzymes (CYP27B1 and CYP24A1) and determine their protein abundances in the cyst wall. We have shown for the first time that 25OHD3 level in the fluid of both cyst types was significantly lower than in preovulatory follicles. Furthermore, we have demonstrated CYP27B1 and CYP24A1 protein immunolocalization and abundance in follicular and follicular lutein cysts. The abundance of protein for both metabolic enzymes was decreased in ovarian cysts when compared to preovulatory follicles. We propose that altered VD metabolism in ovarian cyst might associate with their formation in sows. 相似文献