EDTA辅助小藜修复Pb及Pb-Cd复合污染土壤的研究

通过盆栽试验,研究了Pb及Pb-Cd复合污染土壤添加EDTA(乙二胺四乙酸)对小藜生长和转运、富集Pb、Cd的影响。结果表明,高浓度的EDTA对小藜的生长有抑制作用,Pb及Pb-Cd复合处理下EDTA最佳添加浓度均为2.5mmol·kg-1,此时小藜对Pb的转运系数达2.66和2.41,富集系数达1.51和1.82,分别比对照提高554%和493%、8431%和2367%;对Cd的转运系数达1.87和3.47,富集系数达1.78和10.8,分别比对照提高165%和355%、77%和283%。2.5mmol·kg-1EDTA辅助小藜修复Pb-Cd复合污染土壤的效果优于修复Pb污染土壤的效果。     

昆明小鼠单细胞胚胎体外培养的研究

本试验研究了葡萄糖、EDTA和小鼠血清对昆明小鼠单细胞胚体外发育的影响.结果发现:不含葡萄糖HECM-1组桑椹率为40.05％,对照组为8.14％;含EDTA的CZB组桑椹率为61.18％,对照组为47.44％;小鼠血清对昆明小鼠单细胞胚体外发育无促进作用。可见,葡萄糖抑制昆明小鼠单细胞胚胎体外发育;在不含葡萄糖的条件下,EDTA支持小鼠早期胚胎体外发育;无论有无葡萄糖小鼠血清对小鼠早期胚体外发育无促进作用。     

Plasma calcium, inorganic phosphate and magnesium during hypocalcaemia induced by a standardized EDTA infusion in cows

The intravenous Na2EDTA infusion technique allows effective specific chelation of circulating Ca2+ leading to a progressive hypocalcaemia. Methods previously used were not described in detail and results obtained by monitoring total and free ionic calcium were not comparable due to differences in sampling and analysis. This paper describes a standardized EDTA infusion technique that allowed comparison of the response of calcium, phosphorus and magnesium between 2 groups of experimental cows. The concentration of the Na2EDTA solution was 0.134 mol/l and the flow rate was standardized at 1.2 ml/kg per hour. Involuntary recumbency occurred when ionised calcium dropped to 0.39-0.52 mmol/l due to chelation. An initial fast drop of ionized calcium was observed during the first 20 min of infusion followed by a fluctuation leading to a further drop until recumbency. Pre-infusion [Ca2+] between tests does not correlate with the amount of EDTA required to induce involuntary recumbence. Total calcium concentration measured by atomic absorption remained almost constant during the first 100 min of infusion but declined gradually when the infusion was prolonged. The concentration of inorganic phosphate declined gradually in a fluctuating manner until recumbency. Magnesium concentration remained constant during infusion. Such electrolyte responses during infusion were comparable to those in spontaneous milk fever. The standardized infusion technique might be useful in future experimental studies.     

The effects of different anticoagulants on routine canine plasma biochemistry

The effects of heparin, ethylenediaminetetraacetic acid (EDTA), sodium citrate and sodium fluoride/potassium oxalate on plasma biochemistry results in dogs were studied and compared with serum. Blood specimens from 10 apparently clinical healthy dogs were collected and placed in different tubes containing each anticoagulant tested. Differences in albumin, acetylcholinesterase, ionized calcium and potassium were found between serum and heparinized plasma. Most metabolites and enzymes did not show any variation, but significant decreases in electrolytes, alkaline phosphatase, acetylcholinesterase, bile acids, fructosamine and albumin were found when EDTA was used. Sodium citrate produced a 10-15% decrease in most metabolites and enzymes, possibly due to a sample dilution effect. Sodium fluoride/potassium oxalate produced haemolysis which may have influenced changes in some biochemical parameters.     

Hematologic evaluation of normal and anemic lambs with the Technicon H*1 using EDTA or heparin as anticoagulants

Abstract: A study was performed to evaluate blood from young lambs using the Technicon H*1 hematology analyzer, with emphasis on RBC parameters, comparison of tripotassium EDTA and heparin, and the effects of storage on heparinized blood. Blood samples from lambs 2 days to 18 weeks of age were analyzed within 6 hours, revealing a high precision, except for WBC counts in heparinized blood. The HCT values estimated by the H*1 correlated well (r²= .90) with those obtained by the microhematocrit method. Mean hematologic values obtained for heparinized blood differed by up to 4% from values obtained for blood collected into EDTA. WBC counts decreased 8.4% in heparinized blood stored at 4°C for 1 day, but differences observed in RBC counts were ≤ 2%. Problems occurred when analyzing blood from young lambs with low hemoglobin values because the H*1 incorrectly counted microcytes with volumes of < 10 fL as platelets. When the necessary corrections were performed, the H*1 was useful for analyzing RBC parameters in lamb blood collected both into EDTA and into heparin.     

乳猪料氧化酸败原因分析及其防止措施研究

采用快速贮存法研究了乳猪全价日粮中鱼粉来源、油脂来源和添加高铜、高锌对氧化酸败的影响,并研究了添加不同剂量的 EDTA、乙氧基喹啉及其不同配伍对防止乳猪料氧化酸败的作用。结果表明：①由于乳猪料含有较高的能量和蛋白质,经 60℃快速贮存 3d（相当于常温下贮存 30d）,酸值会成倍增加,乳猪料中添加的油脂和鱼粉会导致贮存后的酸值大幅度地增加,其中添加山东鱼粉的影响较进口鱼粉明显的多;②预混料中高剂量的 ZnO会加剧快速贮存过程的酸败,而 CuSO4· 5H2O的作用则不明显;③ EDTA、乙氧基喹啉均能不同程度地阻抑乳猪料酸败,但后者的作用更为明显,乳猪料中乙氧基喹啉的适宜添加量为 300mg/kg～ 500mg/kg, EDTA、乙氧基喹啉同时配伍使用效果不理想。     

利用EDTA分析大麦发芽过程中蛋白质的溶解

EDTA是一种良好的金属离子螯合剂。本试验分别用不同浓度的EDTA水溶液培养大麦,并与水培养做对比研究。结果表明,EDTA可以通过螯合维持蛋白酶活力所必须的金属离子降低麦芽中蛋白酶活力,并改变麦芽中各蛋白含量。运用SDS-PAGE电泳进一步分析麦芽蛋白,从其组分的变化上分析出贮藏蛋白向可溶性蛋白转化的具体方式。     

锦州市自来水硬度特点及净化对策研究

通过表征锦州自来水,阐述了锦州自来水的特点,主要是高硬度特性,同时为了得到精确数值,采用EDTA络合滴定的方法进行了测定 ,得到总硬度数值为368.4,并采用煮沸法得到了锦州城市自来水的暂时硬度和永久硬度 ,分别为61.2和307.2.通过对数据分析,永久硬度所占比重大,提出了为去除永久硬度,建议采用离子交换吸附法去除.     

超声波辅助EDTA法提取淡水小龙虾壳中甲壳素的工艺研究

以烹饪前后淡水小龙虾壳为原料,研究超声辅助EDTA法提取淡水小龙虾壳中甲壳素的最佳工艺条件,采用EDTA法提取甲壳素,以提取物的灰分含量为评价指标,通过单因素试验和正交试验选择最佳的EDTA浓度、料液比、反应pH值、反应时间,结果表明:EDTA法提取甲壳素的最佳条件为,料液比1∶26(W/V)、EDTA浓度18％、反应时间2.5 h、反应pH值13.在此最佳提取条件下辅助超声波法提取甲壳素,以提取物灰分含量和含氮量作为提取效果的评价指标,筛选最佳超声时间、料液比和超声处理温度,结果表明:超声波辅助EDTA提取甲壳素的最佳工艺条件为处理温度30℃、pH值13、EDTA浓度18％、料液比1∶24(W/V)、超声处理时间45 min、超声频率60kHz、功率180 W.在此条件下,烹饪后甲壳素提取物灰分含量和含氮量均较烹饪前低,分别为0.13％和3.51％.     

钙离子和EDTA对小麦耐锌力的影响

水培结果表明,绵阳11和15号小麦耐有效锌的临界值为600ppm。在0.05—400ppm以内。幼苗生长正常,400ppm时,便出现锌中毒症状,至600ppm时,症状最严重,显症时间最早,从培养第3日起,植株开始枯萎,到第9日,焦叶指数和蔫萎指数均达三级,植株含锌量达6674ppm;而400ppm处理的,症状较轻,植株含锌量明显下降,为6185ppm。加钙处理(500ppmCa~(2+)),使症状加剧,显症提前,植株含锌量相应增高。加0.1％EDTA处理,使症状减轻,推迟显症,植株含锌量下降;在最高锌量(600ppm)加EDTA处理,较其它处理的植株,症状最轻、最迟,含锌量最低。