Development of Nucleic Acid Lateral Flow Immunoassay Detection Method for Foot-and-mouth Disease Virus

In this article, through the combination of nucleic acid probes and immune chromatography, a simple, sensitive and specific detection system——nucleic acid lateral flow immunoassay (NALFIA) for amplifing foot-and-mouth disease virus (FMDV) 3D RT-PCR products was established.An ultrasensitive nucleic acid biosensor (NAB) based on streptavidin-labeled gold nanoparticles dual labels and lateral flow strip biosensor (LFSB) were used in this system.The biotinylated goat anti-rabbit IgG was marked to the NC membrane as the alleged strip and the anti-digoxin antibody was labeled to the NC membrane to capture the digoxin probe.After assemblying gold-labeled strip and detecting RT-PCR products, the detection limit of NALFIA was 0.3×10^-3 to 3×10^-3 μg/μL.The NALFIA was compared with agar gel electrophoresis analysis, the results showed that the sensitivity of NALFIA was higher than agar gel electrophoresis.There was an excellent agreement between the two methods.NALFIA was a method with high sensitive, low cost and short time.In conclusion, this method provided a good alternative to detect FMDV.     

利用QuantStudioTM 3D数字PCR分析转基因玉米MON863含量

QuantStudioTM 3D数字PCR (QuantStudioTM 3D digital PCR,3D-dPCR)是一种基于超高密度亲疏水微孔芯片实现数字PCR分液原理的新型核酸绝对定量平台,在转基因生物定量领域具有极大的应用前景.本研究基于3D-dPCR平台,以转基因玉米(Zea mays)MON863混合样品为例,建立基于单重和双重数字PCR体系的转基因生物(genetically modified organisms,GMOs)含量分析方法.与传统qRT-PCR比较发现,在缺乏样品纯度、纯合度信息的情况下,数字PCR能够较好地排除这些因素的影响,测定准确的量值.研究结果表明,QuantStudioTM 3D数字PCR是一种适用于转基因生物含量分析的精确定量方法,还可反映转基因玉米种子的基因型.本研究基于3D-dPCR建立的转基因玉米MON863单重和双重定量方法为转基因检测提供了新的方法和参考.     

Whole-genome sequence of a novel Chinese cyprinid herpesvirus 3 isolate reveals the existence of a distinct European genotype in East Asia

Cyprinid herpesvirus 3 (CyHV3), also known as koi herpesvirus (KHV), can be subdivided primarily into European and Asian genotypes, which are represented by CyHV3-U or CyHV3-I and CyHV3-J, respectively. In this study, the whole genome sequence of a novel Chinese CyHV3 isolate (GZ11) was determined and annotated. CyHV3-GZ11 genome was found to contain 295,119 nucleotides with 52.9% G/C content, which is highly similar to those of published CyHV3-U, CyHV3-I, and CyHV3-J strains. With reference to CyHV3-U, CyHV3-I, and CyHV3-J, CyHV3-GZ11 was also classified into 164 open reading frames (ORF), which include eight repeated ORFs. On the basis of the 12 alloherpeviruses core genes, results from phylogenetic analysis showed that CyHV3-GZ11 had closer evolutionary relationships with CyHV3-U and CyHV3-I than with CyHV3/KHV-J, which were also supported by genome wide-based single nucleotide substitution analysis and the use of a series of developed molecular markers. This study was the first to reveal the presence of a distinct European CyHV3 genotype in East and Southeast Asia at a whole genome level, which will evoke new insights on exploring the origin, evolution, and epidemiology of the virus.     

鸡胚及禽类细胞系在生物医药领域的应用及研究进展

鸡胚因发育过程清楚，长久以来作为基础和应用科学研究领域重要的实验模型，尤其在鸡胚发育早期绒毛尿囊膜阶段，因其血管丰富，是天然的免疫缺陷宿主，是病理学、药理学和肿瘤学等研究领域的理想实验模型。作者简述了发育早期的鸡胚组织结构，并介绍了鸡胚绒毛尿囊膜在肿瘤研究、血管生成、器官移植、烧伤等疾病机理研究中的应用，以及在鸡胚病理模型基础上进行的抗肿瘤药物筛选的应用。重点介绍了鸡胚及禽类细胞系在病毒繁殖和疫苗生产、治疗性蛋白和单抗生产方面的应用研究进展。多种人源病毒、禽源病毒、支原体等可在鸡胚及禽类细胞上增殖，并用于疫苗生产。作者对常用的禽类纤维原细胞和多能干细胞的发展和特点进行了阐述，并总结了商业化的禽类细胞系来源以及部分易感病毒。鸡胚表达系统能够在目的蛋白特定位点产生人源化糖基，减少目的蛋白对人的过敏反应，且禽蛋廉价易得，可作为生产人用单克隆抗体和治疗性蛋白的合适供体。作者介绍了鸡胚及禽类细胞系在生物医药领域应用的最新进展，并对鸡胚作为动物模型在未来的应用进行了展望。     

不同基因型菠萝蜜种质资源挥发性香气成分分析

以马来西亚1号菠萝蜜、XYS17菠萝蜜、XYS18菠萝蜜和榴莲蜜为材料,采用HS-SPEM-GC-MS进行测定分析,以期揭示不同基因型菠萝蜜资源香气成分组成差异,为资源创新利用提供理论依据。结果表明：4种不同基因型菠萝蜜资源共鉴定出66种香气物质,异戊醇、月桂醛、3-甲基丁酸-2-甲基丁酯、乙酸异戊酯、异戊酸丙酯等9种共有香气成分相对含量存在极显著差异。PCA分析表明,马来西亚1号主要香气成分为3-甲基丁酸戊酯、乙酸丁酯、乙酸异戊酯、异戊酸丁酯和3-甲基丁酸-2-甲基丁酯,XYS17主要香气成分为异戊酸丁酯、3-甲基丁酸-2-甲基丁酯、3-甲基丁酸戊酯、醋酸辛酯和癸醛,XYS18主要香气成分为异戊酸乙酯、异戊酸丁酯、丁酸乙酯、异戊酸甲酯和正辛醛,而榴莲蜜主要香气成分为异戊醇、2-甲基-1-丁醇、3-甲基丁酸-2-甲基丁酯、异戊酸乙酯和异戊酸异戊酯。不同基因型菠萝蜜香气成分物质组成及含量存在一定差异,为今后新品种选育和优异资源开发利用提供参考依据。     

湘桂走廊白背飞虱早期虫源及灾变大气背景

为阐明湘桂走廊白背飞虱早期虫源及大气背景在其北迁过程中的影响,利用HYSPLIT软件模拟白背飞虱灾变性迁入高峰期、始见期后向轨迹,分别从个性及共性角度,分析湘桂走廊白背飞虱主要虫源地及其灾变大气背景。结果表明：（1）1991—2014年湘桂走廊白背飞虱的迁入始见期、首次迁入高峰期分别为0.9 d/10 a、1.6 d/10 a;（2）西南路径是湘桂走廊白背飞虱初始虫源迁入的主要路径,永福站、兴安站和全州站虫源西南路径占比分别为54%、50%、72%;（3）中南半岛白背飞虱借助微弱上升气流完成起飞,850 hPa高度上的强劲的西南气流、丰富的水汽条件有利于其北迁,降水是白背飞虱降落的重要大气动力机制。研究结果为白背飞虱的异地监测及科学防治提供科学依据。     

海南兰科植物新记录

本文报道了海南兰科植物3新记录种,分别是全唇叉柱兰[Cheirostylis takeoi (Hayata) Schltr]、兰屿芋兰（Nervilia lanyuensis S. S. Ying）和台湾隐柱兰（Cryptostylis taiwaniana Masam）。凭证标本存于上海辰山植物园标本馆（CSH）。     

In vitro susceptibility of Brachyspira hyodysenteriae to organic acids and essential oil components

The antibacterial potential of organic acids and essential oil components against Brachyspira hyodysenteriae, the causative pathogen of swine dysentery, was evaluated. Minimum inhibitory concentrations (MIC) of 15 compounds were determined at pH 7.2 and pH 6.0, using a broth microdilution assay. In addition, possible synergism was determined. MIC values for the three tested strains were similar. For organic acids, MIC values at pH 6.0 were lower than at pH 7.2. B. hyodysenteriae was most sensitive to cinnamaldehyde and lauric acid, with MIC values <1.5 mM. Most antibacterial effects of binary combinations were additive, however, for thymol and carvacrol, synergism could be observed. In vitro results demonstrate the antibacterial action of certain essential oil components and organic acids against B. hyodysenteriae.