改进的CTAB法提取胡杨（Populus euphratica）总DNA

以胡杨幼叶为材料,对影响胡杨DNA提取质量的主要因子进行研究,以期建立适宜胡杨DNA提取的优化反应体系,结果表明,在胡杨基因组DNA提取过程中,CTAB缓冲液中增加EDTA的用量及添加完异丙醇沉淀时间是影响胡杨基因组DNA提取的最主要因子。     

生态系统健康评价研究进展

生态系统健康是对生态系统状态特征的一种系统诊断方式。目前,生态系统健康评价是该领域的研究难点,尚处于方法论探索阶段,在研究方法和理论上均有待于进一步发展。该文在国内外关于生态健康理论研究的基础上,重点对生态系统健康评价方法进行归纳分析。生态系统健康评价方法一般包括指示物种法和指标体系法两类方法。指示物种法简便易行,但由于指示物种的筛选标准及其对生态系统健康指示作用的强弱不明确,且未考虑社会经济和人类健康因素,难以全面反映生态系统的健康状况。指标体系法根据生态系统的特征及其服务功能建立指标体系进行定量评价,是目前区域生态系统健康评价的主要方法。并对具体不同评价方法的应用情况和优缺点进行评述。     

油炸食品中丙烯酰胺检测方法的研究进展

丙烯酰胺是高淀粉类食物经油炸处理后产生的小分子有机化合物,具有潜在致癌性.通过介绍油炸食品中污染物丙烯酰胺的检测方法,并对相关检测方法进行简要分析和评述,认为丙烯酰胺的最佳分析方法是GC-MS、LC-MS法,但这两种方法所用的仪器比较昂贵且分析要求高,寻求快速准确且成本低的检测方法是目前丙烯酰胺检测技术方面研究的重点.     

Evaluation of a citrate-based anticoagulant with platelet inhibitory activity for feline blood cell Counts

Abstract: Aggregation of feline platelets in vitro results in difficulty assessing platelet number. A citrate-based anticoagulant containing the platelet inhibitors theophylline, adenosine, and dipyridamole (CTAD; Diatube-H, Becton Dickinson, Oxford, UK) has been developed for use in human platelet studies and heparin assays. To evaluate the efficacy of CTAD in reducing platelet aggregation in feline blood samples, aliquots of blood from 51 cats were anticoagulated with EDTA, CTAD, and for 12 samples, citrate solution. Samples preserved in CTAD had significantly higher (P ≤ .001) platelet counts, as determined by an impedance counter, hemacy-tometer, and smear estimation, than samples preserved in EDTA. In addition, subjective assessment of blood smears showed significantly fewer platelet aggregates (P<.001) in CTAD-treated samples compared with EDTA samples. Although values were similar, automated platelet counts and smear estimates of platelet number were significantly higher (P < .05) and platelet aggregation was significantly less (P < .05) in CTAD samples than in citrate samples. These results suggest that the platelet inhibitory activity of CTAD reduced feline platelet aggregation. Automated total WBC counts in CTAD samples were significantly lower (P<.001) than automated counts in EDTA samples but were similar to manual WBC counts in EDTA samples. Differences in both platelet and WBC counts between CTAD and EDTA or citrate samples were clinically relevant. Mean platelet volume and MCV were significantly lower (P< .05) in CTAD samples than in EDTA samples. No effect was seen on cell morphology or staining characteristics. The anticoagulant CTAD offers an advantage over both EDTA and citrate for feline hematologic analysis, by decreasing pseudothrombocytopenia and pseudoleukocytosis.     

主要晾晒烟调制理论和技术研究进展

综述了我国主要晾晒烟调制的生理、生化基础,调制方法与烟叶质量关系和调制工艺研究,并针对研究现状提出了今后主要晾晒烟调制研究需加强的几个方面。     

高职院校英语教学现状分析及策略

分析了目前高职院校英语教学现状,提出了适合当前英语课堂教学的策略和方法。通过创新教法,最大限度挖掘学生学习潜能,培养他们可持续学习的能力。     

大叶白麻叶总黄酮提取方法研究

通过不同的方法提取大叶白麻叶的总黄酮,比较不同提取方法的提取率及提取物中总黄酮的含量,从而探讨最佳提取方法。     

静海县西瓜设施栽培的演变与实践

通过对静海县西瓜栽培方式演变的分析,论述了不同生产方式下西瓜栽培特点,阐述了设施栽培对西瓜产量、种植效益的影响和对农业增效、农民增收的作用。     

楸树不同嫁接方法对苗木成活率和生长量的影响

为探索出繁育楸树苗木的理想方法。通过劈接、插皮接和带木质部芽接的多组重复试验,研究不同嫁接方法培育的苗木成活率和生长量。在嫁接成活率上,劈接与插皮接有显著性差异,与芽接有极显著性差异,插皮接与芽接差异不显著。芽接与插皮接培育出的楸树苗木在地径和苗高生长上均存在着极显著差异,劈接与插皮接在苗木高生长上存在着极显著差异。带木质部芽接培育的苗木成活率为99%,地径3.15 cm,苗高308 cm。楸树春季用带木质部芽接法培育的苗木成活率高,长势好,应大力推广应用。     

蚂蚁采集方法初探

该文阐述了野外采集蚂蚁的一些常用方法,旨在为有关人员提供一些更加有效的蚂蚁采集方法。