灰飞虱保幼激素结合蛋白基因的克隆及表达动态

克隆了灰飞虱的1个保幼激素结合蛋白(JHBP)基因,命名为LstrJHBP(GenBank登录号:JF728808)。该基因全长1 514 bp,开放阅读框编码289个氨基酸,5’和3’UTR区分别为124和522 bp,预测的相对分子质量为32 599,等电点5.47。综合信号肽预测、氨基酸序列比对及聚类分析结果,推测LstrJHBP是一个细胞质JHBP。qRT-PCR测定表明,LstrJHBP在灰飞虱若虫5个龄期及雌雄成虫均有表达,但4龄若虫期显著高于其他龄期,雌雄成虫间没有显著差异。研究结果为进一步明确灰飞虱的发育及变态调控机制奠定了基础。     

新疆伊犁河谷地区50a来气候变化特征分析

根据伊犁河谷地区10个气象代表站点的气温及降水资料,利用线性趋势函数及t检验法分析了该地区近50 a的气候变化特征。结果表明,伊犁河谷除夏季平均最高气温与年平均最高气温无明显变化趋势外,年平均气温、其他各季平均气温、平均最高气温、平均最低气温均呈上升趋势,平均最低气温上升的幅度远大于平均最高气温的上升幅度,其中,冬季增幅最大,为0.59℃/10 a,秋季次之;暖温年多发生在20世纪80年代后,且近10 a是50 a中最暖的时期,冷温年多发生在20世纪六七十年代;平均气温异常年份为:1969年为异常偏冷年,2010年为异常偏暖年;50 a全年降水量变化呈增湿趋势,变化倾向率为15.4 mm/10 a,其中,夏季降水量为50 a中最多时期,冬季次之,秋季降水量为最少;20世纪六七十年代为枯水期,八九十年代至今为丰水期,但分布不均匀,阶段性干旱时有发生,降水偏多趋势还将继续。     

Waterbird movement across the Great Dividing Range and implications for arbovirus irruption into southern Victoria

Background Waterbirds are the major hosts of various arboviruses. Murray Valley encephalitis virus (MVEV) is an arbovirus native to northern Australia, the major hosts of which are Phalacrocoraciformes (cormorants), Ciconiiformes (herons) and other waterbirds. MVEV is transmitted to humans by mosquitoes and can cause acute encephalomyelitis. In Victoria, MVEV is restricted to the northern side of the Great Dividing Range (GDR), suggesting that waterbirds cannot cross the high country. Methods and results We tested this hypothesis by analysing data on waterbird banding and recovery and discovered that 12 species can cross the GDR. Conclusion Waterbirds have the potential to carry arboviruses, including MVEV, into southern Victoria.     

云南富宁县白背飞虱早期迁入虫源地及降落机制分析

【目的】研究云南富宁县白背飞虱早期迁入虫源地及其降落机制,为白背飞虱的早期预警和有效防控提供依据。【方法】运用大气质点轨迹分析平台HYSPLIT4.8对2010年云南富宁县早期3次灯下主要迁入峰进行了轨迹模拟,并运用气象图形处理软件GrADS对迁飞高峰期进行气象背景分析。【结果】云南富宁县白背飞虱5月上旬开始大规模迁入,迁入高峰期在5月中下旬。2 000m高度轨迹能有效的模拟白背飞虱迁入途径。【结论】云南富宁县白背飞虱早期迁入虫源主要来自缅甸中部,部分来自泰国北部和越南北部。偏南及西南气流为白背飞虱大规模北迁提供了运载气流。风向切变、低温屏障及降雨是迫使白背飞虱大规模集中降落的主要原因。     

Gastrointestinal parasitic worms in equines in the Paraíba Valley, State of São Paulo, Brazil

Over a period of 12 years, from 1988 to 2000, a total of 20 individual equines (16 horses and 4 mules) were selected at random, from 10 municipalities in the Paraíba Valley, in the State of São Paulo, Brazil, and then subjected to necropsy for collection of gastrointestinal worms. Individual samples of 10% of the intestinal contents were also taken for counting and identifying the species present, and to establish the prevalence of worms in equine species in the Paraíba Valley. In the sample considered, the presence of parasites ranged from 155 to 1249 worms. Tapeworms (Cestoidea) were present in about 85% of the animals studied, and roundworms (Nematoda) in 100% of the individuals. All the tapeworms collected were of one single species, Anoplocephala perfoliata. In the case of the roundworms, the prevalence of individual species was: 100% for Cyathostomineae, 90% for Oxyuris equi, 70% for Strongylus vulgaris, 45% for S. edentatus, 15% for Strongylus equinus, 60% for Triodontophorus sp., 50% for Gyalocephalus capitatus, 15% for Oesophagodontus robustus and Craterostomum acuticaudatum, and 5% each for Parascaris equorum, Probstimayria vivipara, Habronema muscae, and Trichostrongylus axei. No specimens of flukes (Trematoda) were found in any of the animals studied.     

新疆额尔齐斯河流域生态承载力研究

通过构建额尔齐斯河流域生态承载力评价指标体系,对各评价指标建立隶属模型,对指标进行正规化处理以消除量纲影响,并利用AHP法求得各指标权重值,利用生态承载力综合评价方法,对额尔齐斯河流域的系统弹性度、资源承载指数和资源承载压力度进行分级评价,最后对额尔齐斯河流域生态承载力进行了综合评价,并提出了建议。     

试论高校图书馆对社会读者实施文献资源共享

社会读者对文献的需求是整个全民文化素质提高的表现形式之一，开展高校图书馆为社会读者服务的研究，开辟为社会读者服务的窗口，是知识经济时代图书馆可持续发展的策略之一。     

大豆花叶病毒的侵染对大豆碳氨化合物代谢的影响

感染大豆花叶病毒（ＳＭＶ）的大豆在发病初期其叶片除还原糖以外，其它各类碳水化合物含量均下降，包括总糖，总可溶性糖、不溶性糖、非还原性糖、但总可溶性蛋白、总游离氨基酸均明显增加．这说明ＳＭＶ侵染明显地抑制了寄主碳水化合物代谢，但促进了寄主氮化合物代谢．因此ＳＭＶ侵染大豆后，在消耗碳水化合物的基础上，优先合成各类蛋白质、这有利于ＳＭＶ的复制．另外在感染ＳＭＶ的大豆中，还发现总氮量增加，这说明ＳＭＶ浸染刺激了寄主对氮的吸收并直接合成各种氨基酸，而有利于病毒的增殖．另一方面由于病毒的增殖不破坏寄主本身的蛋白质，所以氮吸收的增加对寄主起到一种保护作用。     

Phase coupling and spatial synchrony of subpopulations of an endangered dune lizard

Examining demographic phase coupling and spatial synchrony is important for understanding complicated spatiotemporal population dynamics. It is also necessary for protecting rare and endangered species; populations whose dynamics are controlled by resource flux will face increased extinction risk if environmental conditions that drive those resources become spatially synchronized. In this study, we studied the spatial synchrony of subpopulations of the threatened Coachella Valley fringe-toed lizard (Uma inornata), on its remaining sand dune habitat in the Coachella Valley of California. Our results indicated that there is a high level of spatial synchrony between lizard density and their mean reproductive effort for two subpopulations separated by a relatively short distance. High levels of spatial synchrony also exist between the mean lizards’ reproductive effort and annual precipitation. We measured spatial synchrony using four separate methods; using different methods allows understanding of complicated ecological interactions.     

热激处理对枇杷2n花粉发生率的影响

以龙泉1号枇杷为试材,用热激处理诱导小孢子2n配子的发生;并通过观察天然四倍体枇杷花粉,研究其中正常花粉与2n花粉在形态上的异同及其所占的比例。结果表明:30℃/12 h的处理可以使枇杷2n花粉的发生率由对照的0.26％提高到2.64％,败育率约为2.44％。32℃/12 h处理过后的枇杷花粉由于败育率达到20％以上,不能准确计算2n花粉所占比例;在天然四倍体枇杷花粉中存在四种直径不同的花粉,即45μm左右的特大花粉、31μm左右的大花粉。25μm左右的中型花粉和18μm左右的败育花粉,其比例分别为0.1％、54.52％、19.32％和26.06％。