E8启动子是番茄果实成熟乙烯响应性基因E8的启动子区。这个区域中存在着一些响应乙烯以及在果实发育和成熟过程中起调节作用的顺式作用元件,调节E8基因在果实成熟过程中表达(Deikmaa et al.,1998)。本研究根据GenBank中E8基因的启动子区序列设计引物,从番茄基因组中克隆了该启动子,构建了番茄果实特异表达载体,并通过RT-PCR和gus基因检测了该表达载体的果实特异表达活性。 相似文献
Scientific interest in geophysical information about land surface temperature (LST) is ever increasing, as such information provides a base for a large number of applications, including environmental and agricultural monitoring. Therefore, the research of LST retrieval has become a hot topic. Recent availability of Landsat-8 satel- lite imagery provides a new data source for LST retrieval. Hence, exploring an adaptive method with reliable ac- curacy seems to be essential. In this study, basing on features of Landsat-8 TIRS thermal infrared channels, we re-calculated parameters in the atmospheric transmittance empirical models of the existing split-window algorithm, and estimated the ground emissivity with the help of the land cover classification map of the study area. Further- more, a split-window algorithm was rebuilt by virtual of the estimation model of the updated atmospheric transmit- tance and the ground emissivity, and then a remote sensing retrieval for the LST of Shihezi city in Xinjiang Uygur autonomous region of Northwest China was conducted on the basis of this modified algorithm. Finally, precision validation of the new model was implemented by using the MODIS LST products. The results showed that the LST retrieval from Landsat-8 TIRS data based on our algorithm has a higher credibility, and the retrieved LST is more consistent with the MODIS LST products. This indicated that the modified algorithm is suitable for retrieving LST with competitive accuracy. With higher resolutions, Landsat-8 TIRS data may provide more accurate observation for LST retrieval. 相似文献
Comparative tests such as FAT, ELISA, RIA, IEO and CF in the diagnosis of dourine in the horse have proved a satisfactory concordance ratio of the ELISA with CF, which seems to be the most reliable test. Discrepancies have been observed as to the other tests which appear less sensitive than CF test. 相似文献
The latent periods of Visna virus and of progressive pneumonia virus were demonstrated by one-cycle growth studies to end 14–16 hr after infection of sheep choroid plexus cell cultures. Maedi virus was demonstrated to have a 14–18-hr latent period. 相似文献
Human infection by Malassez and Vignal's bacillus (Yersinia pseudotuberculosis) can take many clinical aspects, the most frequent of which is mesenteric adenitis with pseudo-appendicular syndrome, but occasionally also appearing as a tumor of the right lower abdominal quadrant.
In a subsequent stage this mesenteric adenitis is often accompanied by erythema nodosum. There also exist some septicemia-like forms, which have become more and more frequent of late, always appearing on a particular terrain (predisposed persons).
These various forms all correspond to contamination via the digestive route. Other, more exceptional modes of contamination may result in much rarer forms, such as ocular or pulmonary forms.
Diagnosis is based on: (a) demonstrating the presence of Yersinia pseudotuberculosis, (b) serodiagnosis, (c) a positive intradermal reaction, (d) the characteristic aspect of lymph node lesions.
Pseudotuberculosis is extremely frequent in animals. Many species, most of them rodents or birds, can serve as healthy carriers. This is how cats, through their access to this natural “reservoir”, get contaminated and thus act as a “conveyor belt” in transmitting the disease to man. 相似文献