全文获取类型
收费全文 | 17437篇 |
免费 | 684篇 |
国内免费 | 1301篇 |
专业分类
林业 | 710篇 |
农学 | 1090篇 |
基础科学 | 88篇 |
657篇 | |
综合类 | 5169篇 |
农作物 | 765篇 |
水产渔业 | 760篇 |
畜牧兽医 | 6335篇 |
园艺 | 2135篇 |
植物保护 | 1713篇 |
出版年
2024年 | 126篇 |
2023年 | 346篇 |
2022年 | 609篇 |
2021年 | 579篇 |
2020年 | 632篇 |
2019年 | 693篇 |
2018年 | 454篇 |
2017年 | 558篇 |
2016年 | 636篇 |
2015年 | 693篇 |
2014年 | 773篇 |
2013年 | 852篇 |
2012年 | 1092篇 |
2011年 | 1073篇 |
2010年 | 905篇 |
2009年 | 1046篇 |
2008年 | 809篇 |
2007年 | 917篇 |
2006年 | 771篇 |
2005年 | 637篇 |
2004年 | 631篇 |
2003年 | 479篇 |
2002年 | 445篇 |
2001年 | 425篇 |
2000年 | 480篇 |
1999年 | 327篇 |
1998年 | 251篇 |
1997年 | 227篇 |
1996年 | 338篇 |
1995年 | 262篇 |
1994年 | 230篇 |
1993年 | 168篇 |
1992年 | 161篇 |
1991年 | 127篇 |
1990年 | 129篇 |
1989年 | 81篇 |
1988年 | 86篇 |
1987年 | 53篇 |
1986年 | 42篇 |
1985年 | 31篇 |
1984年 | 29篇 |
1983年 | 32篇 |
1982年 | 25篇 |
1981年 | 28篇 |
1980年 | 32篇 |
1979年 | 43篇 |
1978年 | 17篇 |
1977年 | 8篇 |
1956年 | 14篇 |
1955年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 140 毫秒
61.
李厚达 《扬州大学学报(农业与生命科学版)》1987,(4)
用酶联免疫吸附试验(ELISA),对2群鸡的鸡蛋清和1株鸡的马立克氏病疫苗进行检测,发现2群鸡的鸡蛋清中,鸡白血病病毒的阳性率分别是11%和29%,鸡马立克氏病冻干苗隐藏鸡白血病病毒群体特异性(gs)抗原的阳性率为100%。本文指出我国禽苗可能带有鸡的白血病病毒,分析讨论了鸡白血病病毒的垂直传递和水平传播的规律,提出在曾祖代和祖代鸡群中,采用ELISA试验,检测鸡蛋清,能减少以至根除鸡的白血病。 相似文献
62.
Jeanne Dijkstra L. Bos H. J. Bouwmeester Tutung Hadiastono H. Lohuis 《European journal of plant pathology / European Foundation for Plant Pathology》1987,93(3):115-133
Two potyvirus isolates, one from germplasm of yard-long bean (Vigna unguiculata ssp.sesquipedalis) introduced into the Netherlands, and another one from soybean plants (Glycine max) in Indonesia, were compared with two virus isolates of blackeye cowpea mosaic virus (BICMV) from the USA and a Moroccan isolate of cowpea aphid-borne mosaic virus (CAMV). It is proposed that all five isolates be now considered BICMV on the basis of host ranges, symptoms and serology. From our results, and a reassessment of the literature it is suggested to drop the name CAMV in favour of BICMV.Samenvatting Twee potyvirussen, de een in Nederland ingevoerd met genenmateriaal vanVigna unguiculata ssp.sesquipedalis en de ander uit planten van sojaboon (Glycine max) in Indonesië, werden vergeleken met twee isolaten van blackeye cowpea mosiac virus (BICMV) en een Marokkaans isolaat van cowpea aphid-borne mosaic virus (CAMV). Op grond van waardplantenreeksen, symptomen en serologie stellen de auteurs voor om alle vijf isolaten te beschouwen als BICMV. Gebaseerd op de verkregen resultaten en een kritische beschouwing van de literatuur wordt de aanbeveling gedaan om de naam CAMV te laten vallen ten gunste van BICMV. 相似文献
63.
C. Soria M. L. Gomez-Guillamon J. E. Duffus 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(5):289-296
The agent causing a yellowing disease of melon (Cucumis melo), which results in severe losses in crops under plastic on the coastal plains of southeast Spain, was shown to be transmitted in a semipersistent manner by the greenhouse whitefly (Trialeurodes vaporariorum Westwood). The agent was transmitted by grafting, but not by mechanical inoculation or through seeds. The agent was acquired in the minimum period tested (2 h) and could infect plants in an infection feeding interval of 6 h.
Capsella bursa-pastoris, Cucumis melo, C. sativus, Cucurbita moschata, Cichorium endivia, Lactuca sativa andTaraxacum officinale were found susceptible.Results suggest that the yellowing disease affecting melon crops in the southeast of Spain is due to a pathogen similar to beet pseudo yellows virus, but this has to be confirmed by serology. 相似文献
64.
家蚕质型多角体病毒苏州核包涵体小种的纯化 总被引:1,自引:0,他引:1
从BmCPV_t内发现和分离出一株新的核包涵体小种,初步命名为家蚕质型多角体病毒—苏州核包涵体小种(Cytoplasmic Polyhedrosis Virus——Suzhou nuclear polyhedra forming strain,简称CPV——Suzhou N strain),家蚕感梁CPV——Suzhou N Strain引起的多角体病,与国内外已知的CPV各变异株不同。本病蚕的多角体只在中肠组织圆筒形细胞核内检出,多角体内有许多病毒粒子,病毒粒子球状,具有核蛋白紫外吸收特征性光谱和较强的感染活性,病毒核酸初步鉴定为dsRNA。 相似文献
65.
G Castrucci F Frigeri S Ranucci M Ferrari V Cilli B Pedini P Nettleton F Caleffi V Aldrovandi A J Herring 《Comparative immunology, microbiology and infectious diseases》1984,7(1):1-10
Three strains (479 C, 778 TL, 982 LE) of infectious bovine rhinotracheitis (IBR) virus isolated from latently infected calves were compared with the prototype strain of IBR virus (LA strain) in studies which included restriction endonuclease analysis, experimental infection, and reciprocal cross protection tests in cattle. From the restriction endonuclease analysis it appeared that the 3 "latent" viruses were derived from the same isolate, and that it differed slightly from the LA strain. However, latency does not seem to have affected the pathogenicity or the immunogenicity of the virus. This is demonstrated by the identical clinical and virologic response of calves subjected to experimental infection with the various strains under study, and by the finding that when the LA strain and a "latent" strain (982 LE) were tested in cross protection tests in cattle, they proved to be mutually protective. 相似文献
66.
J. F. Antoniw R. F. White 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(6):255-264
This review describes the discovery and identification of the pathogenesis-related proteins (PRs) from tobacco. In crude leaf extracts the PRs are distinguished from the proteins in uninfected plants by their solubility at pH 3, resistance to a range of proteases, and mobility in polyacrylamide gels upon electrophoresis (PAGE) in non-denaturing conditions. PAGE has been used as a qualitative and semi-quantitative assay for PRs, and their migration in gels made from different acrylamide concentrations has been used to identify charge and size isomers and electrophoretically identical PRs in different tobacco cultivars. The subunit composition and molecular weight (mol. wt) of the four PRs identified first in Xanthi-nc were determined by SDS-PAGE; staining the gels has shown that these same four proteins in Samsun NN did not contain carbohydrate, lipid or nucleic acid, nor were they isozymic forms of twenty five enzymes known to increase in activity following infection with TMV. Evidence suggests that most of the PRs in Xanthi-nc and Samsun NN are extracellular.The purification of several PRs from Xanthi-nc, Samsun NN and other tobaccos is described, as well as their mol. wt, subunit and amino acid composition. PRs 1a, b and c consist of a single polypeptide and have similar mol. wt and amino acid compositions. Antisera prepared against purified Xanthi-nc b1 protein have been used to determine serological relationships between PRs and form the basis of a very sensitive quantitative assay using ELISA. The regulation of synthesis of some PRs has been shown to involve translational control. 相似文献
67.
Rob W. Briddon Patricia Lunness Ian D. Bedford Leony C. L. Chamberlin Theo Mesfin Peter G. Markham 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(4):397-400
The cause of a streak disease of pearl millet (Pennisetum glaucum), originating from Nigeria, has been attributed to a geminivirus belonging to the African streak virus cluster. A full-length, infectious clone of the virus was obtained which was transmissible by the vectorCicadulina mbila (Naudé). Analysis of the complete nucleotide sequence of the coat protein gene of this virus shows it to be most closely related to sugarcane streak virus. The possible evolutionary implications of this finding are discussed. 相似文献
68.
M. Angeles Achon Vicente Medina Michael Shanks Peter Markham George P. Lomonossoff 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(2):157-165
In order to characterise and classify an unknown maize-infecting potyvirus isolated from fields in northeast Spain, the entire coat protein gene and the C-terminal twothirds of the large nuclear inclusion protein (NIb) gene were cloned and sequenced. Protein sequencing enabled the cleavage site between the two proteins to be deduced and also revealed that on storage the viral coat protein undergoes a specific degradation in which the N-terminal 39 amino acids are removed. Comparison of the nucleotide sequence of the 3 non-coding region of the viral RNA and the predicted amino acid sequence of the coat protein with the equivalent regions of other members of the potyvirus group revealed that the Spanish virus is closely related to maize dwarf mosaic virus strain A. 相似文献
69.
Pathogenic mechanisms of caprine arthritis-encephalitis virus 总被引:1,自引:0,他引:1
E. G. Mdurvwa P. O. Ogunbiyi H. S. Gakou P. G. Reddy 《Veterinary research communications》1994,18(6):483-490
Goats infected with caprine arthritis-encephalitis virus (CAEV) show chronic arthritis and cachexia, which are progressive in nature. The immunopathogenic mechanisms responsible for these progressive clinical symptoms have not been fully elucidated. Various haematological and immunological parameters were evaluated in experimentally-infected goats showing typical signs of CAEV-induced disease. Infected goats showed recurrent lymphocytosis that may be due to constant presentation of antigen by infected cells of a monocyte/macrophage lineage. The serum alkaline phosphatase and -glutamyl transferase concentrations were elevated in infected goats, a characteristic of hepatic and bone disorders. All other serum chemistry parameters were similar between infected and control goats. Importantly, the serum tumour necrosis factor- (TNF-) levels were higher in infected goats. The cachexia seen in infected goats may be at least partly due to altered metabolism as a result of prolonged elevation of serum TNF- levels. Depressed natural killer cell activity was observed in infected goats and may contribute towards the establishment of a persistent infection with CAEV.Abbreviations AIDS
acquired immunodeficiency syndrome
- CAEV
caprine arthritis-encephalitis virus
- GGT
-glutamyl transferase
- HBSS
Hanks' balanced salt solution
- HIV
human immunodeficiency virus
- NK
natural killer
- PBMC
peripheral blood mononuclear cells
- PCR
polymerase chain reaction
- SAP
serum alkaline phosphatase
- TNF
tumour necrosis factor 相似文献
70.
Movements of bank voles (Clethrionomys glareolus) were studied in a farmland mosaic in Poland. Distances crossed by animals in short-time periods are significantly longer in heterogenous than in homogenous habitats. In long-time and large-spatial scales, a significant portion of the animals in a population travel among habitat elements of the mosaic, reducing the degree of isolation of patch populations and decreasing the probability of local extinction. 相似文献