全文获取类型
收费全文 | 1030篇 |
免费 | 130篇 |
国内免费 | 124篇 |
专业分类
林业 | 21篇 |
农学 | 59篇 |
11篇 | |
综合类 | 282篇 |
农作物 | 46篇 |
水产渔业 | 135篇 |
畜牧兽医 | 348篇 |
园艺 | 6篇 |
植物保护 | 376篇 |
出版年
2024年 | 9篇 |
2023年 | 23篇 |
2022年 | 57篇 |
2021年 | 73篇 |
2020年 | 81篇 |
2019年 | 78篇 |
2018年 | 52篇 |
2017年 | 56篇 |
2016年 | 52篇 |
2015年 | 55篇 |
2014年 | 57篇 |
2013年 | 49篇 |
2012年 | 52篇 |
2011年 | 52篇 |
2010年 | 51篇 |
2009年 | 47篇 |
2008年 | 24篇 |
2007年 | 60篇 |
2006年 | 33篇 |
2005年 | 48篇 |
2004年 | 37篇 |
2003年 | 34篇 |
2002年 | 22篇 |
2001年 | 20篇 |
2000年 | 22篇 |
1999年 | 23篇 |
1998年 | 17篇 |
1997年 | 11篇 |
1996年 | 15篇 |
1995年 | 4篇 |
1994年 | 11篇 |
1993年 | 10篇 |
1992年 | 15篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 5篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1956年 | 5篇 |
1955年 | 4篇 |
排序方式: 共有1284条查询结果,搜索用时 15 毫秒
1.
2.
大肠杆菌毒力因子研究概况 总被引:17,自引:0,他引:17
致病性大肠杆菌的毒力因子主要有黏附素、毒素、外膜蛋白 ( OMP)及铁转运系统等。在大肠杆菌侵袭宿主组织并引起其发病的过程中 ,这些毒力因子相互协调、发挥作用。文章对毒力因子的致病作用、致病机理、免疫原性及其在疾病防制中的应用几个方面的研究概况作了综述 ,为防制大肠杆菌病、研制新型高效的大肠杆菌疫苗提供了理论依据。另外 ,文章还介绍了近年来已相继研制成功的全菌体疫苗、纯化菌毛疫苗、单价或多价基因工程菌毛疫苗以及重组肠毒素双价基因工程苗、OMP亚单位疫苗等。展现了新型疫苗的研制 ,给防制大肠杆菌病带来了新的前景以及大肠杆菌作为工程菌的应用对畜禽其他疾病防制的推动作用 相似文献
3.
The pathogenicity and virulence of ten GreekPseudomonas syringae pv.syringae strains from different hosts (citrus, pear, apple, peach and cherry) were evaluated using three different laboratory methods,
which produced results in good agreement. All ten strains were virulent on apple, pear, cherry and peach trees. The extent
of tissue colonized varied considerably among strains and cultivars. On excised shoots and twigs of apple and pear, strains
BPI 176, BPI 203, PI 2 and PI 14 were the most virulent and strains BPI 689, BPI 992, BPI 4, BPI 20, PI 18 and PI 19 were
the least virulent. On excised shoots and twigs of peach and cherry, strains BPI 176, BPI 203, PI 2, PI 14, PI 18 and PI 19
were the most virulent and strains BPI 4 and BPI 20 were the least virulent. Moderate virulence was evinced by strains BPI
689 and BPI 992. These pathogenicity assays are proposed as rapid and reproducible screening systems to evaluate the susceptibility
of apple, pear, cherry and peach cultivars to this bacterial pathogen. 相似文献
4.
Genetic variability within Phaeoisariopsis griseola from Central America and its implications for resistance breeding of common bean 总被引:1,自引:0,他引:1
The genetic and virulence variability of 112 isolates of Phaeoisariopsis griseola , collected from various locations in Central America, were studied using seven random amplified polymorphic DNA (RAPD) primers and 12 common-bean differential genotypes. Broad molecular diversity ( H = 0·92) among isolates was found using RAPD markers. Fifty pathotypes were identified on 12 differential bean genotypes, 29 of which were represented by only one isolate. Only 18 pathotypes were found in two or more countries. Pathotype 63-63 was the most virulent and caused leaf spots on all 12 common-bean differential genotypes. Comparison of virulence phenotypes and RAPD profiles to known Andean P. griseola isolates confirmed that all isolates belonged to the Mesoamerican group. Pairwise comparison between individual RAPD loci showed that the majority were in gametic phase linkage disequilibrium, revealing that P. griseola maintains a genetic structure that is consistent with asexual reproduction. The molecular and virulence diversities of P. griseola isolates from Central America imply that using single resistance genes to manage angular leaf spot is inadequate and stacking resistance genes may be necessary to manage the disease effectively. 相似文献
5.
Genetic analysis of resistance of wheat seedlings to two races of Puccinia striiformis was conducted on F1 , F2 and F3 generations from crosses Carstens V (CV) × Lee, Spaldings Prolific (SPA) × Lee and CV × SPA. F2 generations from crosses of CV and SPA with Strubes Dickkopf (SD) were also studied. The plants were classified into six resistance classes and analysed by factorial correspondence analysis and nonhierarchical classification. The two P. striiformis isolates tested were a French isolate of race 43E138 and a Lebanese isolate of race 2E16, selected for the differences in their virulence spectra for the common differential cultivars Strubes Dickkopf and Nord Desprez. Resistance of CV and SPA was recessive and dominant to races 43E138 and 2E16, respectively. CV possessed three or four resistance genes, one of them being expressed with both races. Two genes of CV had a cumulative effect for resistance to 43E138 and two or three gave dominant resistance to 2E16. SPA had three resistance genes, all of which gave resistance to 2E16 and two of which also gave resistance to 43E138. SPA had one gene in common with CV for resistance to both races. Furthermore, the gene for resistance to race 2E16 in CV and SPA was allelic with a gene in SD, and was probably Yr25 . 相似文献
6.
Thomas Thomidis 《Phytoparasitica》2002,30(2):191-193
The virulence ofPhytophthora citrophthora isolated from various host-plants on three peach rootstocks (GF677, PR204, KID I) was examined. There was no significant
difference among the rootstocks with respect to their susceptibility to testedP. citrophthora isolates. The most virulent isolate originated from sycamore (Acer pseudoplatanus); isolates from pistachio trees (Pistacia vera) also showed high virulence but were significantly less virulent than the sycamore isolate. Isolates originating from plum
(Prunus domestica), almond (Prunus amygdalus) and lemon (Citrus limon) trees were moderately virulent on peach rootstocks; those from cyclamen (Cyclamen persicum) showed the lowest virulence of those tested. There was thus great variation in virulence among the testedP. citrophthora isolates. It is possible that the isolates ofP. citrophthora from sycamore, pistachio, plum, almond and lemon trees are a threat to peach trees, whereas the low virulence of the isolates
from cyclamen hosts suggests that these pathogens are not a serious threat to peach trees.
http://www.phytoparasitica.org posting Jan. 3, 2002. 相似文献
7.
Genetic variation among asexual progeny of Phytophthora infestans detected with RAPD and AFLP markers 总被引:6,自引:1,他引:6
Genotypic variation among 32 single-zoospore isolates (SZI) of Phytophthora infestans , derived asexually from two hyphal-tip parental isolates (PI-105 and PI-1) of the US-8 genotype, was assessed with 80 random amplified polymorphic DNA (RAPD) primers and 18 amplified fragment length polymorphic DNA (AFLP) primer pairs. In previous investigations, the SZIs from parental isolate PI-105 showed high levels of virulence variability and were differentiated into 14 races, whereas the SZIs from PI-1 showed identical virulence to the parent. The purpose of this investigation was to determine if phenotypic variation observed among SZIs of P. infestans could be detected at the DNA level in these isolates. Polymorphism was detected with 51 RAPD primers and with all 18 AFLP primer pairs in PI-105 SZIs. In SZIs from PI-1, polymorphism was also detected with 25 RAPD primers and 17 AFLP primer pairs. Cluster analysis using the unweighted pair-group method with arithmetic averages (UPGMA) separated the SZIs from parent PI-105 into six virulence groups, 11 RAPD groups and three AFLP groups. Cluster analysis of PI-1 SZIs, which all belong to the same virulence group, differentiated them into four RAPD groups and six AFLP groups. No close correlation among RAPD, AFLP and virulence groups could be established within the two progenies of SZIs. Results of this study suggest that there is a considerable level of inherent genetic variability among SZIs derived asexually from the same parental isolate. The possible mechanisms and implications of this genetic variation are discussed. 相似文献
8.
Henk-jan Schoonbeek Johannes G.M. van Nistelrooy Maarten A. de Waard 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(9):1003-1011
The role of multiple ATP-binding cassette (ABC) and major facilitator superfamily (MFS) transporter genes from the plant pathogenic fungus Botrytis cinerea in protection against natural fungitoxic compounds was studied by expression analysis and phenotyping of gene-replacement mutants. The expression of 11 ABC (BcatrA–BcatrK) and three MFS genes (Bcmfs1, Bcmfs2 and Bcmfs4) was studied. All genes showed a low basal level of expression, but were differentially induced by treatment with cycloheximide and the plant defence compounds camptothecin, eugenol, psoralen, resveratrol and rishitin. The latter compounds induced expression of BcatrB at a high level. Eugenol was more toxic to BcatrB gene-replacement mutants than to the control isolates. Eugenol also caused an instantaneous increase in mycelial accumulation of the fungicide fludioxonil, a known substrate of BcatrB. However, there was no difference in virulence between the wild-type and BcatrB gene-replacement mutants on Ocimum basilicum, a plant known to contain eugenol. The results indicate that BcatrB is a transporter of lipophilic compounds, such as eugenol, but its role in virulence remains uncertain. 相似文献
9.
将蜡蚧轮枝菌Vp菌株在PDA培养基上23℃黑暗培养,分别于第5、15、20、25、30天后采收。将不同时间采收的分生孢子置于2%葡萄糖培养液内培养,16 h后检测其萌发率,结果表明,采收于第5天和第15天的分生孢子萌发率分别达到89.76%和94.98%,显著高于采收于第25天(30.27%)和第30天(6.12%)的孢子的萌发率;在PDA培养基培养5 d和15 d的分生孢子在相同浓度下对温室白粉虱的致病力分别达到83.40%和74.44%,显著高于培养了30 d的分生孢子的致病力(8.76%)。 相似文献
10.
During 1997–2000 the virulence variation and distribution of virulence phenotypes of Bremia lactucae (lettuce downy mildew) in natural populations of Lactuca serriola (prickly lettuce) were studied. Altogether 139 isolates of B. lactucae originating from the Czech Republic, France and Germany were examined for the presence of 27 virulence factors (v-factors) and their combinations. In the Czech population, 37 different v-phenotypes (P1–P37) of B. lactucae were found to occur on L. serriola . Most v-phenotypes were characterized by v-factors that match resistance ( Dm genes/R-factors) carried by L. serriola . A wide diversity of v-phenotypes was recorded every year, but most were rare and did not reappear in other populations of B. lactucae . The three v-phenotypes P21, P28 and P29 were most frequent and widely distributed. There was variation both between spatially isolated populations and within populations of the pathogen. Geographic differences in virulence were found for the southern parts of Moravia, where the phenotypic composition of pathogen populations was completely different from the remaining part of the area investigated. However, some populations had v-phenotypes similar to those of spatially distant populations in Moravia. 相似文献