向日葵副产物的营养特性及在反刍动物中的应用

饲料资源缺乏一直以来都是制约中国畜牧业发展的主要因素。从长远看,常规饲料已不能维持中国畜牧业的可持续发展,开发新的饲料资源是解决饲料不足的重要途径之一。向日葵副产物营养价值较高、来源广、价格低,是一种非常具有开发价值的新型饲料。向日葵副产物包括向日葵饼粕、向日葵盘、向日葵秸秆及葵花籽壳：向日葵饼粕蛋白质含量高、纤维含量低,是一种优质的植物蛋白来源,可以提高奶牛乳汁的营养价值、促进肉牛生长、提高羊的瘤胃发酵效果;向日葵盘粗脂肪含量高,含有大量的膳食纤维,可以提高奶牛的产奶量和乳脂率,促进肉牛增重,提高羊瘤胃挥发性脂肪酸浓度以及粗蛋白质、粗纤维、中性洗涤纤维的消化率;向日葵秸秆富含氮、磷、钾、钙、镁等矿物质元素以及少量的蛋白质,可以为肉牛提供一些常量元素和微量元素,提高羊的日增重,降低料重比;葵花籽壳的主要成分是纤维素和木质素,含有丰富的生物活性物质,可以提高奶牛日增重、肉牛饲料转化率。作者具体介绍了向日葵副产物饼粕、葵盘、秸秆、葵花籽壳的营养价值,以及目前它们在反刍动物饲料中的应用情况,以期为今后向日葵副产物的开发利用提供理论依据。     

不同生长条件下切花向日葵生产效益和碳足迹分析

为探究切花向日葵生产碳足迹和经济效益,通过调研切花向日葵生产企业,利用生命周期评价法评估露地,拱棚和日光温室3种不同生长条件下切花向日葵碳排放,核算生产成本和效益,结果表明：露地切花向日葵生产碳足迹为2174.46kg/hm2,生产成本51994元/ hm2,收入178608元/ hm2;拱棚向日葵生产碳足迹为2041.13kg/ hm2,生产成本59443元/ hm2,收入175973元/ hm2;日光温室向日葵生产碳足迹为2038.92kg/ hm2,生产成本58465元/ hm2,收入179544元/ hm2。3种不同生长条件下切花向日葵生产的主要成本均来自人工,种子,地租和肥料,碳排放最主要的来源均为厩肥和氮肥。在未来切花向日葵生产中,可通过优化生产技术提高肥料利用率,研发专用机械降低劳动力投入,增加轮作等途径来实现减排增收的目的。     

Seed viability determination in parasitic broomrapes (Orobanche and Phelipanche) using fluorescein diacetate staining

Fluorescein diacetate (FDA) was investigated as a potential viability stain for seeds of holoparasitic broomrapes Orobanche and Phelipanche (Orobanchaceae), using four weedy and two non-weedy taxa. FDA viabilities were compared with the currently used 2,3,5-triphenyl tetrazolium chloride (TTC) test, and in vitro germination induced by the strigolactone analogue GR-24. Viable FDA-stained seeds were characterised by bright yellow–green fluorescence under 450–490 nm blue light. These viable seeds could be discriminated from non-viable seeds, in which low levels of fluorescence, similar to non-stained control seeds were observed. FDA viabilities were not significantly different from those obtained using the TTC stain; however, viability values for both stains were consistently higher than levels of artificially induced germination. Positive TTC-staining showed continuous variation that was difficult to interpret. Nevertheless, the TTC test does not involve grinding seeds, and therefore probably remains a practical alternative to FDA for screening soil samples contaminated with Orobanche and Phelipanche seeds, which indicates different applications for the two viability tests. Interestingly, the strigolactone analogue GR-24 only induced germination in O. crenata , P. ramosa and O. minor , suggesting a high specificity towards this germination inducer among the species investigated. We suggest that FDA-staining provides a potential alternative to the currently used TTC test for seed viability assays for Orobanche and Phelipanche , and other parasitic weeds which are an obstacle to crop cultivation.     

Accumulation of soluble phenolic compounds in sunflower capitula correlates with resistance to Sclerotinia sclerotiorum

Disease symptoms and total soluble phenolics content have been analysed in four sunflower (Helianthus annuus L.)lines with different resistance levels(from highly susceptible to resistant) to head rot caused by Sclerotinia sclerotiorum (Lib.) de Bary. At the beginning of the flowering stage, capitula were inoculated by spraying with a water suspension of ascospores, and disease symptoms were evaluated from day 6 to day14 after inoculation. The most susceptible genotypes showed all their ovaries to be necrosed and abundant lesions in corollas, bracts and receptacle. In the resistant line, the ovary and corolla were only partially necrosed with no symptoms in the bracts or the receptacle. Total soluble phenolics were extracted and quantified from different parts of the capitulum in both inoculated and non-inoculated plants. The amount of phenolic compounds depended on the sunflower line, the time after inoculation, and the tissue. Higher constitutive and induced phenolic content as well as phenylalanine ammonia-lyase activity were present in the most resistant line, these differences correlated with the absence/presence of disease symptoms. This revised version was published online in July 2006 with corrections to the Cover Date.     

压砂西瓜间套马铃薯油葵栽培试验

针对宁夏压砂西瓜产业发展的需要,探索压砂西瓜高效栽培模式技术(间套)。结果表明:压砂西瓜套种油葵共生期长,前期油葵与西瓜争光和肥水,西瓜坐瓜和大小受限制,影响西瓜产量;压砂西瓜套种马铃薯共生期长,马铃薯与西瓜争光、争肥水不明显,西瓜坐瓜和大小不受限制,西瓜产量不受影响,每667 m2套种马铃薯生产比对照多收入231.2元。     

Sclerotinia resistance in sunflower: I. Genotypic variations of hybrids in three environments of Argentina

Sclerotinia sclerotiorum infections on sunflower capitula produce white rot, one of the most dangerous diseases sunflower bears in all humid areas. Therefore, disease quantification on developed genetic materials is a very important action in sunflower breeding. Given the horizontal type of resistance these evaluations should be made in different environments. Several hybrids obtained after crossing seven female × seven male sunflower inbred lines in a factorial mating design were evaluated for resistance to white rot in two locations in the southeast of the province of Buenos Aires, Argentina. Disease incidence and the symptom appearance index indicated significant genotypic effects and genotype × environment (G × E) interactions. Genotypic effects were a greater source of variance than the G × E interaction effects. The G × E interactions only represented changes in magnitude. These results were used to identify the best genotypes for both resistance factors. Four hybrids showed low disease incidence values under both artificial and natural infections, of which only two had high values of the symptom appearance index at both locations. These hybrids are considered to have adequate resistance attributes for the ascosporic penetration and the mycelium extension in the capitulum tissue and could therefore be sown in the province of Buenos Aires without increasing risks of S. sclerotiorum attacks.     

不同油葵种植方式对银北灌区盐碱地土壤温度、含水率和电导率的影响

为了探究油葵不同种植方式对盐碱地土壤水、热、盐分布状况的影响,以平播（T1）为对照,开展了半膜平播（T2）、半膜平播+膜间覆秸秆（T3）、起垄沟播（T4）、起垄沟播+沟覆秸秆（T5）、垄膜沟播（T6）、垄膜沟播+沟覆秸秆（T7）等6种种植方式对油葵田土壤温度、水分和电导率的影响研究。结果表明：与对照相比,不同种植方式均提高了早上7:00时5cm深度的土温,但油葵花期土壤增温效果明显弱于苗期,垄膜沟播种植方式更有利于减缓地 表5cm深度土温的剧烈变化。与对照相比,在油葵苗期,T7和T6的0~20cm土层土壤含水率分别提高了6.30%和5.01%,20~40cm土层分别提高了6.00%和3.38%,在油葵开花期,土壤含水率较对照提高幅度有所降低。与播种前0~20cm土层土壤电导率相比,T6在苗期和开花期均有所下降,其中开花期下降了33.81%,T7在苗期略有增加,在开花期则略有下降,其他处理在苗期增加幅度较大。因此,在银北灌区油葵生产中,垄膜沟播是一种适宜的油葵种植方式。     

Precision phenotyping of imidazolinone-induced chlorosis in sunflower

Chlorosis level is a useful parameter to assess imidazolinone resistance in sunflower (Helianthus annuus L.). The aim of this study was to quantify chlorosis through two different methods in sunflower plantlets treated with imazapyr. The genotypes used in this study were two inbred lines reported to be different in their resistance to imidazolinones. Chlorosis was evaluated by spectrophotometrical quantification of photosynthetic leaf pigments and by a bioinformatics-based color analysis. A protocol for pigment extraction was presented which improved pigment stability. Chlorophyll amount decreased significantly when both genotypes were treated with 10 μM of imazapyr. Leaf color was characterized using Tomato Analyzer^® color test software. A significant positive correlation between color reduction and chlorophyll concentration was found. It suggests that leaf color measurement could be an accurate method to estimate chlorosis and infer chlorophyll levels in sunflower plants. These results highlight a strong relationship between imidazolinone-induced chlorosis and variations in leaf color and in chlorophyll concentration. Both methods are quantitative, rapid, simple, and reproducible. Thus, they could be useful tools for phenotyping and screening large number of plants when breeding for imidazolinone resistance in this species.