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中草药添加剂饲喂猪肉中CPK,GPI活性和肉质关系的研究 总被引:9,自引:0,他引:9
对添加中草药和常规抗生素试验猪的背最长肌进行相关酶测定,发现磷酸肌酸激酶(CPK),磷酸葡萄糖异构酶(GPI)的活性,试验组明显高于对照组,分别提高了30.54%和20.04%(P<0.05).屠宰及肉质试验表明,试验组的瘦肉率及眼肌面积分别比对照组提高5.79%和8.48%(P<0.05),而脂肪率、膘厚、失水率和贮存损失则分别下降31.40%,28.21%,50.03%和20.35%(P<0.05). 相似文献
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Masahiro Hatamoto Ryo Aizawa Kogomi Koda Toshiki Fukuchi 《Journal of Pesticide Science》2021,46(2):198
Aminopyrifen is a novel 2-aminonicotinate fungicide with unique chemistry and a novel mode of action. The fungicide showed high antifungal activity mainly against Ascomycetes and its related anamorphic fungi under in vitro and pot conditions (EC50 values: 0.0039–0.23 mg/L and 1.2–12 mg/L, respectively). The active ingredient strongly inhibited germ-tube elongation of Botrytis cinerea below 0.1 mg/L and invasion into a plant. The compound exhibited no cross-resistance to commercial fungicides in B. cinerea. The antifungal agent showed high preventive efficacy and translaminar action. In the field, aminopyrifen controlled gray mold and powdery mildew at 150 mg/L. Our findings suggest that aminopyrifen is useful for protecting crops from various plant pathogens. 相似文献
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采用水平淀粉凝胶电泳方法对虾夷马粪海胆(Strongylocentrotus intermedius)、刺参(Apostichopus japonicus Selenka)、半滑舌鳎(Cynoglossus semilaevis)、文蛤(Meretrix meretrix)和虾夷扇贝(Pecten yessoensis)不同组织中的葡萄糖磷酸变位酶(PGM)和磷酸葡萄糖异构酶(GPI)进行了检测分析。结果发现,虾夷马粪海胆肌肉组织和成熟性腺组织中的PGM有较强的表达活性和表达位点差异,两种组织中GPI活性稍差。刺参的肠道组织和肌肉组织只有PGM和GPI表达活性的差异,而无位点差异。半滑舌鳎肌肉组织检测到两个PGM位点,三个GPI位点。文蛤闭壳肌组织PGM表现出非常高的多态性,而GPI表现为单态。虾夷扇贝闭壳肌组织的PGM和GPI各只检测到一个位点,遗传变异程度比较低。以PGM和GPI为指标,对我国北方5种重要海水养殖品种的同工酶遗传变异水平和其它相关遗传参数进行了分析,对群体遗传结构可能的变化规律以及与某些性状的相关性进行了探讨,这些参数对它们的遗传选育有一定的指导作用。 相似文献
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根据乳酸乳球菌密码子的偏好性,在不改变编码蛋白的基础上,优化设计并合成枯草芽孢杆菌磷脂酰肌醇特异性磷脂酶C基因,将其与大肠埃希菌 乳酸乳球菌穿梭质粒pAMJ399连接,构建重组质粒pAMJ399 PIPLC,并电转化至乳酸乳球菌中进行诱导表达。SDS PAGE分析显示:重组蛋白以可溶性蛋白的形式分泌于胞外,分子质量约35 ku,与预期蛋白大小一致。重组蛋白在PI 李斯特氏菌显色平板上显现明显的乳白色晕圈,证明重组蛋白具有酶活性,磷脂酰肌醇特异性磷脂酶C(PI PLC)在重组乳酸乳球菌中成功获得表达。通过优化培养条件,以2%转接量,在含有1%红霉素抗性的GM17液体培养基中,于32 ℃静止培养24 h,测得培养基上清液中PI PLC的浓度为1.092 μg·mL-1。 相似文献
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A 2193-nucleotide cDNA encoding acetylcholinesterase (AChE) of the stable fly, Stomoxys calcitrans (L.) was sequenced and expressed in the baculovirus system. The open reading frame encoded a 91 amino acid secretion signal peptide and a 613 amino acid mature protein with 96% and 94% identity to the AChEs of Haematobia irritans (L.) and Musca domestica (L.), respectively. Structural characteristics of M. domestica, H. irritans, and Drosophila melanogaster AChEs were conserved in the S. calcitrans AChE. The recombinant enzyme was inhibited by eserine, coroxon, and paraoxon and exhibited Km values of 63.9 μM for acetylthiocholine and 96.7 μM for butyrylthiocholine, confirming its biochemical identity as an acetylcholinesterase (EC 3.1.1.7). These data will enable rapid identification and assay for mutations that reduce AChE sensitivity to organophosphate (OP) pesticides, potentially aiding resistance management efforts to prevent fixation of the mutations in pest populations. 相似文献
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