豫麦10号高产栽培技术调查总结

通过对小麦新品种豫麦1O号的观察,认为该品种在上蔡县增产潜力较大。为充分发挥其增产优势,从整地播种,施肥浇水,中、后期管理等方面提出了措施和建议。     

小麦新品种银春10号综合评价及应用前景

银春10号是白银市农业科学研究所通过种子卫星搭载,经太空诱变,多年选育而成的春小麦新品种,于2017年通过甘肃省农作物品种审定,2021年通过国家农作物品种审定。银春10号株型紧凑,穗层整齐,具有高产稳产,适应性广,产量构成三要素协调等特点,适宜在西北水地春麦区种植,有较广阔的推广应用前景。     

大豆新品种宝豆10号特征特性与良种繁育技术

宝豆10号具有茎秆坚韧、籽粒较大、蛋白含量高、抗逆性强等优点,适合陕西省夏播地区种植。审定后,宝豆10号生产经营权得到转让,种植面积不断扩大。为确保宝豆10号种子纯度不降低,提高宝豆10号种子纯度和制种产量,本文介绍了大豆新品种宝豆10号的特征特性,提纯复壮技术,以及包括播前准备、播种与施肥、田间管理、病虫害防治、去杂去劣、适时收获和储藏等关键环节的良种繁育技术,旨在推进大豆新品种宝豆10号的示范与推广     

Thr-Ser-Ser位点对猪BCL10募集功能的影响

在完整克隆出猪BCL10基因的基础上,用大引物PCR法构建3个真核表达载体：pEGFP-B（完整猪BCL10）载体、pEGFP-D（缺失CARD结构域）载体和pEGFP-P（缺失Thr-Ser-Ser位点）载体,转染猪血管内皮细胞（SU-VEC）,观察其表达情况,以此研究Thr-Ser-Ser位点（富含TS区）对猪BCL10募集功能的影响。结果显示,pEGFP-B仅在胞质中表达,并呈短棒状,而pEGFP-D与pEGFP-P则在胞质和核内均有表达。表明CARD结构域参与猪BCL10募集过程,且Thr-Ser-Ser为CARD结构域中关键氨基酸位点,这为进一步研究猪BCL10功能和作用机制奠定了基础。     

Hmgn3和Hoxa10对小鼠子宫基质细胞蜕膜化过程中Foxo1表达的调控

利用小鼠子宫基质细胞体外诱导蜕膜化模型转染Hmgn3或Hoxa10过表达载体及siRNA片段,通过荧光定量PCR方法检测Hmgn3和Hoxa10对Foxo1表达的调控。结果显示:转染Hmgn3或Hoxa10过表达载体可促进Foxo1在子宫基质细胞蜕膜化过程中的表达,而转染Hmgn3或Hoxa10siRNA则可抑制Foxo1的表达。在小鼠子宫基质细胞中转染Hmgn3或Hoxa10siRNA后再添加孕酮,Foxo1的表达显著下降。同样,干扰Hmgn3或Hoxa10也可减低cAMP对Foxo1表达的调控。结果表明:Hmgn3和Hoxa10可通过Foxo1来影响小鼠子宫基质细胞的蜕膜化,孕酮和cAMP可通过Hmgn3和Hoxa10来调控Foxo1在子宫基质细胞中的表达。     

High‐level exogenous trans10, cis12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells

Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75–150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P < 0.05), but did not have inhibiting action on cell proliferation (P > 0.05). Treatment with 150 μmol/L trans10, cis12 CLA for 48 h resulted in a 17.1% reduction (P < 0.0001) of medium chain fatty acids (MCFA, C14 < C < C16), a 26.5% reduction (P < 0.0001) of unsaturated fatty acids (UFA) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A (acetylCoA) carboxylase (ACC) (P = 0.046), fatty acid synthase (FAS) (P = 0.017) and stearoylCoA desaturase1 (SCD1) (P = 0.002). Another finding was that trans10, cis12 CLA elevated expression of diacylglycerol acyltransferase2 (DGAT2) (P = 0.020) and long chain acylCoA synthetases (ACSL) (P = 0.032). In conclusion, higher trans10, cis12 CLA, not low trans10, cis12 CLA, inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS, ACC, SCD1, DGAT2 and ACSL.     

黄土丘陵区白羊草与达乌里胡枝子混播草地土壤呼吸日变化特征

土壤呼吸是反映土壤质量和肥力性状的重要指标,阐明禾―豆混播草地土壤呼吸作用的变化规律及其影响因素,可以为准确评估混播草地的环境效应及响应提供依据。在2011和2012年6月份,采用动态密闭气室分析法,比较了白羊草(Bothriochloa ischaemum)与达乌里胡枝子(Lespedeza davurica)不同比例间作混播草地总土壤呼吸速率的日变化特征、混播植物贡献率以及温度敏感性等。结果表明,不同混播比例草地的总土壤呼吸速率日变化为单峰型曲线,峰值出现在12:00~14:00。总土壤呼吸速率日均值高低顺序为单播白羊草白羊草和达乌里胡枝子间作单播达乌里胡枝子。不同间作比例草地的总土壤呼吸速率与白昼气温相关显著(P0.05),而与5、10和15 cm土层白昼土壤温度关系不明显;单播条件下,白羊草草丛的土壤呼吸对温度的敏感性高于达乌里胡枝子;各草地土壤呼吸Q10值的变化范围为1.48~2.61,以单播白羊草最高,单播达乌里胡枝子和两者间作混播草地间无显著差异(P0.05)。     

Effects of Wnt10b on dermal papilla cells via the canonical Wnt/β‐catenin signalling pathway in the Angora rabbit

Wnt10b is a member of Wnt family that plays a variety of roles in biological functions, including those in the development of hair follicles. To investigate the effect of Wnt10b on hair growth in the Angora rabbit and to determine the underlying molecular mechanism, we cultured dermal papilla (DP) cells with exogenous Wnt10b in vitro. We observed the expressions of downstream critical gene β‐catenin and lymphoid enhancer‐binding factor 1 (LEF1) in Wnt/β‐catenin pathway. The levels of β‐catenin mRNA and protein were higher in the Wnt10b group of DP cells than in the Control group, and the mRNA level of LEF1 in the Wnt10b group was higher than in the Control group. Moreover, translocation of β‐catenin from cytoplasm to nucleus was activated in the Wnt10b group. Furthermore, the mRNA levels of the hair follicle‐regulatory genes, insulin‐like growth factor‐1 (IGF‐1) and alkaline phosphatase (ALP), and the protein activity of ALP was also upregulated in the Wnt10b group compared to their corresponding levels in the Control group. These data suggest that Wnt10b could activate the canonical Wnt/β‐catenin signalling pathway to induce DP cells in the Angora rabbit. In addition, the proliferation of DP cells was significantly promoted when cultured with Wnt10b for 48 and 72 hr, suggesting that Wnt10b plays a pivotal role in the proliferation and maintenance of DP cells in vitro. In conclusion, this study demonstrates that Wnt10b may promote hair follicle growth in Angora rabbit through the canonical Wnt/β‐catenin signalling pathway that promotes the proliferation of DP cells.