青贮玉米蠕形菌的分离鉴定及其致病性分析

本研究为初步明确引起青贮玉米(Zea Mays L.)叶斑病的蠕形菌种类,采集疑似病斑叶片样本,对病原菌进行分离纯化,形态特征观察,ITS-rDNA序列和GADPH基因序列系统发育分析及致病性测定。形态学和分子生物学鉴定结果表明分离得到的142株病原菌为3属6种真菌,其中麦根腐平脐蠕孢(Bipolaris sorokiniana)和玉米生平脐蠕孢(B.zeicola)为优势病原菌,分别为77株和48株;其次为大斑病菌(Exserohilum turcicum)9株,疣状弯孢(Curvularia verruculosa)2株,新月弯孢(C.lunata)3株,穗状弯孢(C.spicifera)3株。致病性测定发现麦根腐平脐蠕孢和玉米生平脐蠕孢对青海省8个主栽青贮玉米品种均有致病性,其余4种病原菌的致病性各有差异。本研究初步明确引起青贮玉米叶斑病的蠕形菌种类及其致病性,为后续开展病害诊断及其综合防控奠定了基础。     

A Spot Price Model with Var Cost

This paper has applied the var cost to the theory of spot price.An augmented Lagrange method is employed to develop the exact model of spot price of active power and reactive power,and the physical means of Lagrange multipliers are explained.The numerical examples are given to demonstrate the validity of the proposed model.     

猕猴桃溃疡病生防芽孢杆菌B2的鉴定及应用

猕猴桃溃疡病是一种毁灭性细菌病害,该病发展迅速,防治难度大,严重制约了猕猴桃产业的发展。为找到一种有效防治该病的方法,针对化学防治易产生抗药性、污染环境、杀伤天敌等特点,从猕猴桃根际土壤中分离共获得25株芽孢杆菌,采用点菌法筛选出4株对猕猴桃溃疡病菌(Pseudomonas syringae pv.actinidiae)具有拮抗效果的菌株(抑菌圈直径达到5 mm以上),其中B2拮抗作用最强。根据菌落(体)形态特征、生理生化鉴定以及16S r DNA序列分析,初步鉴定B2为蜡样芽孢杆菌(Bacillus cereus)。将B2的发酵原液稀释成1×109cfu/m L的发酵液,并在此基础上依次配制50、100、500、1000、2000倍的稀释液,用于盆栽试验。盆栽试验采用喷雾法,30天后的防治效果分别达到了90.45%、86.52%、80.90%、67.98%、47.19%、15.73%,60天后尽管病斑有少量增加,旧病斑有所增大,但与对照相比,病斑增长速度明显减缓。大田试验结果证明,该菌具有较好的生防潜力,喷雾法选择B2发酵液的50倍稀释液,涂抹法选择B2发酵液的100倍稀释液,可有效降低猕猴桃溃疡病的危害。     

Quality and maximum profit of industrial tomato as affected by distribution uniformity of drip irrigation system

The tomato industry reformed its system of payment by weight of tomato, introducing a corrective system based on percent level of fruit dry matter produced. Such a decision implies significant changes in the management of irrigation systems, with a need to emphasize the technological quality of the marketable product. Three levels of distribution uniformity of the irrigation system are analysed, and related production functions of crop yield and percent of dry matter are presented as well as their use on the optimisation of dry matter, expected revenues and seasonal applied water. Results are critically influenced by the distribution uniformity. They demonstrate the inter-relationship between crop production, percent fruit dry matter and irrigation management, and the importance of considering non-uniformity in the economic analysis of industrial tomato production. Decreases in uniformity lead to a reduction in dry matter production per unit land. Decreases in dry matter are also observed with increasing levels of seasonally applied water, with the optimal level always lower than the required for maximum yield. Such interaction suggests a continuous and inverse relationship between profit and water applied. However, due to the corrective system of payment, by levels of percent of dry matter produced, for some uniformity, the expected revenue follows the yield-water production function instead of the dry matter function. This fact introduces disturbances in the optimal water applied inducing higher than expected levels of water applied for profit maximisation. The simulated data also show that incentives to switch to new systems or management practices able to raise the distribution uniformity result more from profit losses than increases in water price.     

Outbreak of shoot blight and dieback of Eucalyptus spp., caused by Pseudoplagiostoma eucalypti in Brazil

An outbreak of a new and severe disease was observed in Eucalyptus plantations of Bahia state, Brazil. An Ascomycota fungus has been frequently associated with the main symptoms of the disease namely leaf spot, branch cankers, shoot blight, defoliation, and dieback. Based on morphological characteristics, phylogenetic analysis (ITS and TEF-1α genes), and pathogenicity test on Eucalyptus plants, Pseudoplagiostoma eucalypti was identified as the causal agent of the disease. Although P. eucalytpi has been known from in Brazil since 1998, this is the first report of it causing severe disease and die-back on Eucalyptus spp. and we also record new symptoms associated with the pathogen.     

‘福山包头’大白菜叶柄黑点病发生特点及抗性分析

以‘福山包头’大白菜为试材,利用田间调查、显微观察和叶片离体培养的方法,对‘福山包头’系列大白菜叶柄黑点病的发生特点及抗性进行了研究,以期为改善‘福山包头’大白菜抗病性及新品种选育提供参考依据。结果表明:(1)大白菜叶柄黑点病主要发生于叶柄表皮细胞且起始于细胞周质或细胞壁,该病可发生于大白菜的整个生育期,且进入包心期后发展较快;(2)氮素能诱导叶柄黑点病的发生,且铵态氮较硝态氮诱导效果更明显;(3)氮素浓度与叶柄黑点病的发病程度呈正相关,但氮素并不是影响叶柄黑点病发生的唯一因素;(4)‘福山包头’系列大白菜种质资源中,‘福东2号’‘福山82’对叶柄黑点病的抗性较强,‘福小87-13-13’‘皱福’的抗性较弱。     

高压静电预处理技术对番茄保鲜的影响

研究了高压静电预处理后的番茄在保鲜过程中的呼吸强度、抗压强度以及失重情况的变化规律。结果表明，利用电场强度为150kV/m的高压静电场预处理番茄45min后，可使自然条件下番茄的呼吸高峰推迟4d后出现，并发现高压静电预处理能有效地保持番茄较高的表面抗压强度和较低的失重率，延长番茄的贮藏保鲜时间。