Effects of CENP-W down-regulation on human glioma U87 cells

AIM: To study the effect of centromere protein W (CENP-W) down-regulation on human glioma U87 cells.METHODS: Small interfering RNA (siRNA) was used to inhibit the expression of CENP-W in the U87 cells. The interference effect of siRNA was evaluated by RT-qPCR and Western blot. The proliferation of the cells was analyzed by MTT assay, BrdU staining and colony formation experiment. Transwell chamber assay was used to detect the invasion ability of the cells. The cell migration ability was measured by a scratch test. The changes of the cell cycle distribution and apoptosis were analyzed by flow cytometry.RESULTS: The results of MTT assay, colony formation experiment and BrdU staining showed that the cell proliferation and colony formation abilities in experimental group were significantly lower than those in control group and negative control group. The results of Transwell and scratch experiments showed that the migration and invasion abilities in experimental group were weaker than those in blank control group and negative control group. The results of flow cytometry analysis showed that the cell cycle distribution in experimental group was arrested in G₀/G₁ phase. The percentage of apoptotic cells in experimental group was higher than that in control group (P<0.05).CONCLUSION: Down-regulation of CENP-W expression inhibits the proliferation, migration and invasion of human glioma cells and promotes the apoptosis of the cells, suggesting that CENP-W may be a potential target of gene therapy for human glioma.     

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.     

微囊型甲醛捕捉剂对薄木饰面人造板甲醛释放量的影响

随着薄木饰面板在家具、装修领域中的使用量越来越大,人造板的游离甲醛释放问题也愈发引起人们关注,现有的甲醛捕捉剂一般只能在短期内发挥效果,而对人造板甲醛的长期释放控制效果并不理想。为解决人造板甲醛释放的长期性问题,以尿素为芯材、乙基纤维素为壁材制备微囊型甲醛捕捉剂,探讨不同乳化剂及芯壁比制备的微囊型甲醛捕捉剂对饰面人造板甲醛控释效果的影响。结果表明,当乳化剂为十二烷基硫酸钠、芯壁比为3∶1,乳化剂用量1%、壁材质量分数3%、搅拌速度1000 r/min时,制备出的微胶囊芯材负载效果最好,负载量达到36.24%、负载效率为8.05%。采用该微胶囊以2%的质量比与脲醛树脂胶黏剂混配进行饰面板贴面热压时,得到的薄木贴面板的甲醛释放量相比未处理饰面板降低了37.9%。经过20 d后,微胶囊对其饰面板甲醛的释放仍表现出一定的控制效果,饰面板的甲醛释放量为0.99 mg/L,仅为未处理饰面板的56.9%。说明用微囊型甲醛捕捉剂对饰面人造板进行处理不仅能够对饰面板中的甲醛进行控释,而且在长期的放置、使用过程中也能够持续发挥作用,从而达到降低室内空气中甲醛浓度的目的。     

一种复合微生物菌剂在甜脆豌豆中的田间应用效果

为了探究复合微生物菌剂615在甜脆豌豆栽培中的田间应用效果,以甜脆豌豆品种‘长寿仁’为试材,研究了4种施肥处理（不施肥对照,单施复合菌剂615,单施化肥,化肥+复合菌剂615配施）对主要农艺性状、产量和品质、根际微生物数量的影响。结果表明：所有施肥处理可显著提高结荚率（幅度为7.90%～12.49%）,单施菌剂的病害率减少10.83%;所有施肥处理均可显著增加小区产量（幅度为10.75%～19.82%）,其中化肥与菌剂配施增幅最大(19.82%)。单施菌剂使鲜食籽粒的总糖、蛋白和淀粉含量增加50.00%、9.24%和59.37%(p<0.05),其开花结荚期根际中的细菌、真菌和放线菌数量是苗期的1.93倍、2.38倍和2.36倍,化肥与菌剂配施可提高总糖和淀粉含量(p<0.05)。综合经济和生态效益,甜脆豌豆施肥推荐化肥与复合菌剂615配施。     

干旱胁迫下保水剂对雪茄烟叶碳氮代谢和中性香气物质含量的影响

为研究干旱胁迫下保水剂种类和用量对雪茄烟叶碳氮代谢和香气质量的影响,以德雪3号为试材,采用盆栽试验研究不同保水剂种类(SAP保水剂、沃特保水剂和自制保水剂TS-PAA)与用量(2、4、6 g·株^-1)下的雪茄烟叶碳氮代谢和中性致香成分含量变化。结果表明：1)使用保水剂可有效提高雪茄烟生长发育前期(45 d以前)氮代谢强度,加速氮素营养向烟碱的转化与积累,削弱后期(60 d以后)氮代谢强度,使烟碱的积累程度明显减弱,75 d时各处理的烟碱含量较对照均有大幅度下降,以TS-PAA 2 g·株^-1处理降幅最大,为32.26%。2)使用保水剂能加速中后期(45 d以后)碳代谢进程,促进雪茄烟叶中淀粉、总糖、还原糖含量增加,其中以TS-PAA 4 g·株^-1处理增幅最高,较CK增幅分别为327.28%、80.51%、79.54%,糖碱比增幅161.90%,两糖比(还原糖/总糖)均维持在0.9左右。3)使用保水剂还能显著延迟叶片衰老,提升烟株的抗旱能力,促进雪茄烟叶中性致香成分积累。4)在干旱协迫条件下,保水剂的使用有利于促进雪茄烟...     

微生物菌剂浸种联合生物菌肥防控玉米茎腐病的应用效果

采用微生物菌剂浸种后撒施生物菌肥M1、M2预防玉米茎腐病,检测对玉米田土壤微生物量及酶活性的应用效果。结果表明,五谷丰素浸种联合菌肥M1、M2撒施处理显著提高防控玉米茎腐病的应用效果,乳熟期的预防效果分别为66.3%和68.1%,蜡熟期的预防效果分别为72.9%和74.6%,显著促进玉米出苗率、株高、有效穗数、穗长、穗重、百粒重以及产量。土壤中的微生物碳、微生物氮以及土壤碱性磷酸酶、脲酶、蛋白酶、多酚氧化酶活性均显著增加,其中,五谷丰素浸种联合M2菌肥处理后土壤微生物氮达64.2 mg/kg,脲酶活性达3.3 mg/(g·h),蛋白酶活性达86.5μg/(g·h),多酚氧化酶活性达111.6μg/(g·h)。     

保鲜剂对香石竹切花生理生化特性的影响

试验表明,2种含有不同物质的保鲜剂均能不同程度地增加香石竹切花鲜重和花径,改善体内水分状况,延缓可溶性蛋白的降解,延长瓶插寿命,其中以保鲜剂1(2%S+200mg·L-1 8-HQ+150mL AC+50 mg·L-1 Al2(SO4)3+100 mg·L-1VC+50 mg·L-1 SA)的效果较好,属环保型保鲜剂.     

不同抗旱保水剂及其用量对高粱保水效果及产量的影响

为了探讨不同抗旱保水剂及其用量对高粱保水性能及产量的影响,采用陕西杨凌抗旱保水剂和河北唐山抗旱保水剂1～2 kg/667m2在高粱上试验.结果表明,陕西杨凌抗旱保水剂保水效果优于河北唐山抗旱保水剂,且以陕西杨凌抗旱保水剂施2 kg/667m2对高粱土壤保水效果较好;高粱产量以处理3(陕西杨凌抗旱保水剂施2 kg/667m2)最高,为346.4 kg/667m2,较对照增产13.2%.     

ZTC1+1-Ⅱ澄清剂对银杏叶醇提液的絮凝工艺研究

[目的]采用ZTC1＋1-Ⅱ型天然澄清剂对银杏（GinkgobilobaLinn.）叶醇提液进行絮凝提取,优化生产工艺,提高药液的澄清度和药液质量。[方法]ZTC1＋1-Ⅱ型澄清剂组分A、B2种。用蒸馏水和1%醋酸溶液配制成1%的溶液,预溶胀24h,用双层纱布过滤,即得澄清剂组分A或B。之后,以絮凝物重量为衡量指标,研究不同影响因素对絮凝效果的影响。[结果]确定了ZTC1＋1-Ⅱ型天然澄清剂对银杏叶醇提液进行絮凝的最佳工艺条件为：澄清剂组分加入次序为先加组分B后加组分A,银杏叶醇提液pH值为5.5,100ml醇提液中组分B、A的用量分别为1.6、0.8g/L,澄清温度为40℃,澄清时间为40min。[结论]该研究可简化后续工艺程序和降低生产成本。     

红壤旱地施用保水剂和稻草覆盖对花生生长和产量的效应

在江西丘陵红壤坡地上进行的试验结果表明:施用保水剂和稻草覆盖均可促进花生的生长发育,明显提高花生的产量,但对花生的出苗率影响不大.施用保水剂、覆盖稻草以及两者叠加处理分别比空白对照增加花生产量26.6%、7.69%和29.1%,其中以单施保水剂处理的经济效益最佳,比对照增加纯收入6330.60元/hm2.