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91.
A detailed study has been conducted to evaluate the residues of endosulfan and its principal metabolite (alpha-endosulfan, beta-endosulfan and endosulfan sulfate) which may have accumulated in environmental samples due to regular aerial spray application of endosulfan on cashew leaf plantation for a period of 20 years. Three months after the last spray of endosulfan 350 g litre-1 EC at 300 ml acre-1 (equivalent to 105 g AI acre-1 = 42.5 g ha-1), a total of 93 samples of cow milk, fish, water, soil and dried cashew leaf were collected from a village in Kasargode District, Kerala, India, where endosulfan contamination was likely to have occurred. All the samples were analyzed for total residues of endosulfan (comprising alpha- and beta-endosulfan), endosulfan sulfate and also the potential hydrolysis product endosulfan diol, using gas chromatography with electron capture detection. The minimum detection limits of total endosulfan was 0.001 microgram g-1. Analysis of soil samples showed the deposition of total endosulfan residues in the range < 0.001-0.010 microgram g-1, and dried leaf samples showed residues of endosulfan in the range < 0.001-3.43 micrograms g-1 dry weight. In cow milk, fish and water, endosulfan residues could not be detected above the minimum detection limit. Endosulfan diol was not observed in any sample. The data obtained was confirmed by GC-MS-EI using selective ion monitoring (SIM) mode. 相似文献
92.
Antonious GF 《Pest management science》2002,58(1):85-91
The efficacy of esfenvalerate (84 g litre-1 EC; Asana XL) at 7.0 g AI ha-1 on broccoli was tested against the flea beetle, Phyllotreta cruciferae Goeze (Chrysomelidae: Coleoptera) and the imported cabbage worm, Pieris rapae L (Pieridae: Lepidoptera) under field conditions. Insect populations were monitored before and after treatment. Periodic sweep-net collections and examination of the leaves in treated and untreated broccoli plots revealed mean reductions of P cruciferae levels of nearly 98% 1 week post-application compared with untreated plots. The residual toxicity of esfenvalerate was also effective for 2 weeks in reducing population density of P rapae by 69% on broccoli leaves. The impact of esfenvalerate on feeding damage to broccoli leaves was established by counting the number of feeding holes made by P cruciferae on spring broccoli and P rapae on fall broccoli. As the leaf area ingested increased, a linear relationship was seen between the number of holes and number of insects. Results indicated that forage destruction by the two pests was significantly reduced by esfenvalerate application. Esfenvalerate was extracted from broccoli at 1 h and 1, 3, 7, 10, and 14 days post-application for residue analysis. Residues on spring broccoli were 12.2, 5.2 and 2.9 micrograms cm-2 on the leaves and 0.13, 0.05 and 0.02 microgram g-1 on the heads at 1 h, 1 and 3 days, respectively. Only trace levels (0.001 microgram g-1) were detected in/on the heads 14 days after spraying. On the basis of half-life (T1/2) values, persistence of esfenvalerate on spring broccoli leaves (T1/2 = 1 day) was shorter than on fall broccoli (T1/2 = 1.6 days). T1/2 values were 2.1 and 3.6 days on spring and fall broccoli heads, respectively. The implications of these residue levels on re-entry times into treated fields are discussed. 相似文献
93.
鸡肝脏和肌肉组织中常山酮残留的ELISA检测 总被引:3,自引:0,他引:3
将常山酮进行人工改造,制备了半抗原常山酮琥珀酸衍生物(Hal-suc).采用N-羟基琥珀酰亚胺活性酯法将半抗原与牛血清白蛋白(BSA)、卵清白蛋白(OVA)偶联,制备免疫原和包被原.动物免疫6次后采血制备抗血清,以间接ELISA法测定血清效价,测得抗血清的最佳工作浓度为1∶102 400.建立了常山酮在鸡肝脏和肌肉组织中检测的ELISA法,该方法在50、100、500 ng/g 的添加浓度水平下,在鸡肝脏和肌肉组织中测得的平均回收率范围分别为74.2%~96.8%和74.3%~90.0%,检测限分别为28和19 ng/g. 相似文献
94.
建立了氧氟沙星在肉鸡组织内残留的高效液相色谱分析法。采用荧光检测器,激发波长278nm,发射波长445nm。色谱柱为Hypersil BDS—C18,柱温40℃;以磷酸(三乙胺调pH至2.5)-乙腈(86:14)为流动相,流速1.0mL/min;进样量为20μL。氧氟沙星在肌肉、肝脏和肾脏中的平均回收率分别为96.3%、87.5%和76.4%,检测限为10ng/g,在10~1000ng/mL浓度范围内,氧氟沙星的峰面积与其浓度呈良好的线性相关,达到了氧氟沙星残留分析的要求。利用建立的方法对氧氟沙星在肉鸡体内的残留消除规律进行了研究,以1L水添加500mg的剂量,对120只肉鸡连续给药5d,对不同停药时间所取肌肉、肝脏和肾脏样品中的药物残留进行分析。结果表明,氧氟沙星在肉仔鸡组织中的残留消除速度较为缓慢,至休药23d时,仍可在肝脏和肾脏分别检出39和35μg/kg的氧氟沙星残留。在本实验的条件下,建议氧氟沙星临床休药期以25d为宜。 相似文献
95.
论述和分析木质剩余物复合材料老化的原因,主要有木质剩余物的老化、胶粘剂的老化、木质剩余物的回弹和膨胀—收缩应力。综合论述木质剩余物复合材料加速老化试验方法,指出木质剩余物复合材料在加速老化试验方面的研究,适宜采用ASTM D1037六循环加速老化法和老化仪检侧法。分析木质剩余物复合材料老化的影响因素,如温度、湿度、试件尺寸等。 相似文献
96.
97.
QuEChERS-超高效液相色谱-质谱联用法测定熊胆粉中9种苯二氮䓬类药物残留量 总被引:1,自引:0,他引:1
建立了超高效液相色谱-质谱联用法(UPLC-MS/MS)快速测定中药材熊胆粉中9种苯二氮?类药物残留的方法。样品以乙腈为提取溶剂,经萃取盐包除杂提取,采用分散式固相萃取管 (EMR-Lipid dSPE) 净化,ACQUITY BEH C18色谱柱(1.7 μm,2.1×100 mm)分离,多反应监测模式(MRM)采集数据,外标法定量。在优化的条件下,虽9种药物存在不同程度的基质效应,但不影响检测结果的准确性。方法学考察结果显示,9种苯二氮?类药物在对应的浓度范围内线性关系良好,决定系数(R2)≥0.9961,方法检出限(LOD,S/N =3)在0.06~0.71 μg/kg范围内,定量限(LOQ,S/N =10)在0.20~2.60 μg/kg范围内,3个加标水平的平均回收率在69.1%~108.0%范围内,日内RSD为1.8%~9.0%,日间RSD为2.6%~9.3%。该方法前处理操作简便、重现性好,准确度高,为熊胆粉中苯二氮?类药物残留的风险监控提供了一种有效的检测技术。 相似文献
98.
99.
One way of meeting the supply demands of the wood industry is through the reduction of waste in timber processing. It has
been estimated in Ghana that for every tree felled, nearly 50% of the tree volume is left in the forest in the form of branches,
crownwood, and stumps. In this study, the potential of utilizing branchwood as raw material from logging residues to help
meet the timber demands of the downstream processing sector was analyzed experimentally. The sawing characteristics of the
branchwood of Aningeria robusta and Terminalia ivorensis with diameters in the range 10–25 cm using live sawing and cant sawing methods were determined using a randomized complete
block design, 2 × 4 factorial with three replications. Experimental results indicate that, the relative lumber value yields
for first and second grade boards of A. robusta and T. ivorensis branches were 25 and 20%, respectively, while the green lumber volume yields were 40 and 32%, respectively. A high proportion
of sapwood and the occurrence of washboarding in T. ivorensis contributed to that species’ relatively low lumber value and volume yield. The experimental results further indicate that
the sawing technique used had no significant influence on lumber yields. However, at 5% level of significance, significant
differences in lumber yield existed between some of the diameter classes. 相似文献
100.
据样地调查,该25年生杉木人工林群落的植物有41科84属120种,群落的外貌特征以包括藤本在内的高位芽占绝对优势,叶特征以革质,单叶,中小型叶为主,由于该群落的幼树层以米储和木荷等占优势,若让其自然发展或杉木林采伐后不炼山,并排除其它人为干扰,该群落将向与常绿阔叶林采伐前的群落类型相似的方向演替。 相似文献