Effects of Interspecific Competition on the Inheritance of Quantitative Traits in White Clover *)

Analysis of a half diallel cross among four white clover cultivars, grown as monocultures and in mixtures (duocultures) with two separate perennial rye-grass cultivars, confirmed that for stolon and leaf number a higher proportion of the genetic variance in duocultures was non-additive. For canopy height and dry weight, however, this proportion was less affected by inter-specific competition. An additive-dominance model of gene action sufficed for all four characters in monocultures, but in duocultures epis-tasis was just one of the factors complicating the genetic picture. This, coupled with short-term reversals in the direction of dominance, merely illustrates the complexity of breeding white clover cultivars. Possible future developments in the breeding of white clover are discussed in the light of these results.     

Introduction of a self-compatible gene of Lolium temulentum L. to perennial ryegrass (Lolium perenne L.) for the purpose of the production of inbred lines of perennial ryegrass

Interspecific hybrids between self-compatible species, Lolium temulentum L. and self-incompatible species, L. perenne L. were obtained using embryo rescue. Two cycles of backcrossing of interspecific hybrids with L. perenne were carried out. A 1: 1 segregation ratio of self comatibility and incompatibility was observed in backcross generations. These segregation data confirmed that self-compatibility of L. temulentum was controlled by a single gene. It suggested that the self-compatible gene of L. temulentum could be introduced to self-incompatible plants of L. perenne through interspecific hybridization. It appears that utilization of a self-compatible gene of L. temulentum would be useful for production of inbred lines of L. perenne. The possibility of breeding procedures of perennial ryegrass using a self-compatible gene was discussed. This revised version was published online in August 2006 with corrections to the Cover Date.     

Useful variation in in vitro digestibility within perennial ryegrass

In vitro dry matter digestibility (DMD) and dry matter yield (DMY) of herbage from plots sown with three selected perennial ryegrass varieties with similar heading dates (Ba11778, Talbot and Gator) under a range of harvesting frequencies (2, 3, 4 and 5-weekly harvests) were compared over two harvest years (1994 and 1995). Varieties varied in mean percentage DMD over all harvests and harvesting frequencies by 3.4 and 3.5 units in 1994 and 1995 respectively. Varietal ranking in mean DMD over all harvests was the same under each harvesting frequency in both years: Ba11778 being the highest, Gator the lowest and Talbot intermediate. In 1995, Gator had a higher proportion of unsown species in the herbage than both Ba11778 and Talbot but this was only a minor factor contributing to the differences among the varieties in mean DMD over all harvests. Although varieties consistently ranked in the same order of mean DMD over all harvesting frequencies during 13 5-week periods, the range among varieties varied from less than 1 unit to 8.6 units. This range was greatest in mid-season of each year when mean DMD over all varieties was lowest. The minimum mean DMD over all harvesting frequencies during both years was 75.5%, 70.9% and 66.8% for Ba11778, Talbot and Gator respectively. The most digestible variety Ba11778 also had higher annual DMY than the other two varieties in both harvest years and under all four harvesting frequencies. These results indicate that there is sufficient useful genetic variation for DMD within perennial ryegrass to justify breeding for the trait and its routine assessment in official variety trials.     

Evaluation of genetic variation among wild rice populations in Cambodia

A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources.     

Co-adaptation between neighbours? A case study with Lolium perenne genotypes

Summary Co-adaptation between neighbouring genotypes of Lolium perenne was examined in a pot experiment over an 18 month period. Competitive interactions in pure and mixed stands were examined using 9 genotype pairs (established as neighbours from electrophoresis data), 7 from a 10 year-old, and 2 from a 40+ year-old pasture. Combinations of each pair of neighbours grown together, and each grown with 5 randomly chosen alien (non-neighbour) genotypes sampled from the same pastures were also assessed. Seven harvests of green matter produced above 20 mm were made, and 50–135 kg N ha^-1 was administered after each harvest. Data for dry weight at each harvest for pure and mixed stands, and Relative Yield Totals (RYT) were obtained for neighbours and aliens in each treatment.In 6 of the 9 pairs of neighbours grown as pure stands, the yields of neighbours did not differ significantly at the final harvest. In 8 of the 9 pairs grown as mixtures of pairs of neighbours, the yield of one of the neighbours was significantly reduced and one neighbour had been eliminated. Over the last 5 harvests, yields from (alien + neighbour) mixtures had the greatest dry matter yields. No suppression of one component by the other was recorded for this (alien + neighbour) combination.Based upon RYT estimates, neighbours did not yield more when grown together than when grown separately with aliens. There was no relationship between original genotype size in their fields of origin and dry matter yield in this experiment.This experiment provides no evidence for he evolution of co-adaptation between surviving neighbours in the two pastures from which these individuals were sampled. The genotypic architecture of pasture would seem to be the product of random and/or competitive exclusion of individual genotypes, rather than the evolution of co-adapted groups of individuals through time. The consequences of extreme depletion of genetic variation in the first two years after establishment, the very low rates of seedling establishment in established pastures, and the impact of the grazing animal would suggest that there is little likelihood of or potential for, co-adaptation evolving between neighbours.     

Variation in digestibility in nine populations of perennial ryegrass (Lolium perenne) and its genetic basis

Summary Thirty five individuals from each of nine perennial ryegrass populations were sampled from set-stocked and zero-grazed swards and from an old permanent pasture. The swards were 6, 18, 30 months and 11 years old when sampled. The permanent pasture was at least 40 years old.Dry matter digestibility (DMD) and organic matter digestibility (OMD) were determined for green leaf material harvested 4 days after ear emergence. Significant differences were found between the populations, but genotypic differences were found only for OMD. The largest proportion of the total variation was due to differences between blocks. In the zero-grazed populations, DMD and OMD declined significantly with increasing sward age. This was not found for the set-stocked swards. Two sets of pair crosses, from which mid-parent, progeny regressions could be determined, were carried out.The regressions of offspring on mid-parent for set-stocked and zero-grazed swards were used to assess the presence of additive genetic variation and to estimate narrow sense heritability of DMD. The coefficients of correlation, 0.122 and 0.226 respectively, were non-significant, indicating an absence of additive genetic variation.     

Diploids and natural autotetraploids in the predominantly vegetatively propagated Brassica oleracea L. var. ramosa DC and their cytology

Summary Perennial kale is a rare vegetable and forage crop in the Dutch province of Limburg where it is disappearing. It may be a relic of a primitive kale form which could have been grown on a large scale in West-Europe. The crop is predominantly vegetatively propagated.Cytological investigations indicated that a number of accessions are autotetraploid with 2n=4x=36. The origin and age of these autotetraploid accessions are not known, but they may stem from adventitious buds on wounded tissue during the vegetative propagation.     

60种花卉多酚、黄酮含量及其抗氧化活性

为了筛选出抗氧化能力强的花卉品种,对60种花卉植物的50％乙醇提取物进行了多酚、黄酮含量测定,并对提取物进行清除DPPH的试验.结果表明:花卉中含有丰富的多酚和黄酮类物质,多酚含量在70 mg/g以上的花卉有芍药花、金盏菊、黄刺玫、美蔷薇、红玫瑰等,黄酮含量较高的花卉有连翘、马蹄莲、山楂、杏、榆叶梅等,含量在7.05～...     

多年不结果的冬枣树优质高产栽培技术

冬枣树要求栽培技术严格,管理不好或一些技术配合不好就不结果。为了解决多年生冬枣树不结果的问题,在天津市静海县、西青区、宁河县等地对多年不结果的冬枣树进行了技术示范,达到了优质高产的目的。本文主要介绍多年不结果冬枣树的肥水管理、提高坐果率的技术措施、冬季修剪及病虫害防治技术。