致谢审稿专家

Two agricultural soils were collected from Dahu and Pinchen counties and swine manure compost (SMC) from Pingtung County in Taiwan, China to investigate the sorption and dissipation of three tetracyclines (TCs), i.e., oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC), in compost, soils and soil/compost mixtures with different organic carbon (OC) contents. There were seven treatments in total. TCs were most strongly adsorbed to SMC in all treatments due to the high OC content. When SMC was present in the soils, the sorption of TCs was significantly enhanced, which might be attributed to the increased OC content and CEC. The adsorption of TCs showed non-linear adsorption isotherms and fitted well to the Freundlich model. After 49 d of incubation at 25 oC in soils and soil/compost mixtures in the dark, TCs elapsed in all substrates, with the time required for 50% degradation (DT50) between 20 and 41 d, and the time for 90% degradation (DT90) between 68 and 137 d. Soil amended with compost enhanced the stability of TCs and reduced their mobility. The dissipation of TCs in a soil environment was slow, indicating that these compounds might be persistent in soil.     

稻壳发电残余物稻壳灰对有机物的吸附作用

为解决稻壳发电残余物——稻壳灰的利用问题,该文研究了稻壳灰在不同条件下对有机污染物的吸附作用。结果表明,稻壳灰在经过碱洗处理后,其对有机污染物的吸附性能明显提高。当溶液pH值小于7.0时,稻壳灰对有机污染物的吸附效果要好于pH值大于7.0时的吸附效果。该研究表明,稻壳灰经过碱洗处理后,可以用作吸附剂,吸附污水中的有机污染物,实现废物的再次利用。     

添加竹酢液和菌剂对园林废弃物堆肥理化性质的影响

为研究不同水平竹酢液及菌剂对堆肥效果的影响,以园林废弃物为原料,通过L9(34)正交设计以竹酢液和菌剂为添加剂进行静态好氧高温堆肥试验,分析了堆肥过程中各个时期不同处理的温度、pH值、EC值、全N、全P和全K的变化趋势,并对腐熟后各处理堆肥产品中的全N、全P、全K、Mg、Fe和S的质量分数进行了方差分析与多重比较。结果表明,添加竹酢液和菌剂的处理使堆肥初期温度上升较快,有效降低了堆肥产品中的pH值和EC值;添加竹酢液和菌剂对于堆肥产品中的全N、全P、全K、Fe和S质量分数均影响极显著,可有效增加全P、全K、Fe和S质量分数,且存在交互作用;氮以氨气形式挥发损失,与空白比较,加入菌剂会加速氮的损失,而添加一定稀释倍数的竹酢液可有效保氮;将堆肥产品间各指标进行比较,稀释1 000倍竹酢液2 L+0.5%“有机废物发酵菌曲”的园林废弃物堆肥效果 最好。     

堆肥过程中水溶性有机物常规荧光与固体表面荧光光谱比较

为研究固体表面荧光表征堆肥有机质结构及物质转化特征的可行性,采集堆肥原样,提取水溶性有机物,分别对两者的含碳量及前者的固体表面荧光和后者的常规荧光光谱进行分析。结果显示：堆肥过程中,水溶性有机物占总堆肥有机物的比例不断下降,第40天降至5.1 g/kg。堆肥进行时,水溶性有机物的发射、激发光谱及同步荧光光谱中的类腐殖质荧光的强度均不断增强,而堆肥原样的激发、发射和同步3种扫描形式的固体表面荧光却不断降低。3种荧光光谱中,堆肥原样和水溶性有机物的同步荧光光谱在常规荧光光谱和固体表面荧光中都出现了类蛋白和类腐     

松嫩平原玉米带农田土壤有机碳时空格局

该文基于吉林省第二次全国土壤普查省数据、县级土壤剖面资料和2003－2006年实测数据,估算了不同土壤类型农田表层土壤有机碳密度和储量,并对近25年来土壤有机碳时空变化特征及其原因进行分析。结果表明,总体上松嫩平原玉米带农田土壤有机碳密度和储量呈增加趋势。其中,碱土、暗棕壤、黑土和草甸土的土壤有机碳密度增幅分别达33%（4.16 kg/m2）,23.05%（3.79 kg/m2）、16.51%（3.74 kg/m2）和12.20%（3.77 kg/m2）;相反,黑钙土有机碳密度下降幅度达30.79%（2.18 kg/m2）。两时期土壤有机碳密度的空间分布格局基本一致,呈中部高、边缘低的趋势,但25年间土壤有机碳含量变化与1980年初始含量呈显著负相关（r＝-0.615**,P＜0.01）,且4.04 kg/m2是土壤有机碳上升或下降的临界值。根据West等提出的土壤碳汇潜力估算方法,如果保持1980年土地利用方式和传统的栽培耕作措施不变的情况下,松嫩平原玉米带农田土壤有机碳的碳汇潜力为0.33 Tg/a。     

耕地土壤有机质空间变异性的随机模拟

有机质是土壤重要的肥力特征,研究盐渍土改良区耕地土壤有机质空间变异特征可为土壤质量提升提供科学依据。以山东省禹城市盐渍土改良区典型地块耕地土壤有机质为研究对象,在全面野外调查和室内化验分析以获得大量的土壤有机质相关信息的基础上,运用地统计学方法对有机质进行了序贯高斯模拟各次实现（SGSV）、序贯高斯模拟平均实现（SGSA）和ordinary Kriging插值（OK）,并将SGSV、SGSA、OK与实测数据进行了统计参数、变异函数、空间分布趋势等方面进行了对比分析。结果表明OK、SGSA改变了有机质数据的空间结构,具有“平滑”效应,SGSA在消除平滑影响方面优于Kriging插值;SGSV具有与实测数据相同的空间自相关结构,对预测点的模拟值具有不确定性,为揭示研究区域土壤有机质的空间结构特征提供了有力的工具,对盐渍土改良区土壤有机质空间不确定性的风险研究具有更实际的意义。     

How well does a botanical garden collection of a rare palm capture the genetic variation in a wild population?

Conservation is increasingly central to the botanic garden mission. Living plant collections are important components of conservation. Critical evaluation of living conservation collections with population genetic analysis can directly inform ex situ conservation strategy. Here, we quantify the degree of genetic variation captured through a population-based collection protocol, and explore optimal sampling for ex situ conservation. An extensive living collection derived from one population of Leucothrinax morrisii (Arecaceae) provided a model system. We compared 58 specimens from the ex situ collection with 100 individuals from throughout the parent population via 6 ISSR loci. Random bootstrapped resamples of the data were made to model differently structured ex situ collections. Mean diversity (He) differed little between the collection (0.204) and the population (0.216), and genetic distance (D) was very close (0.036). Very few private alleles were found between the collection and the population. Allelic capture, as measured by percent of private alleles, was greater than 94%. Resampled collections of different sizes captured from 48% to 94% of alleles. Pairwise comparison of bootstrapped resamples suggests that increasing the representation of half-sibling groups does not significantly increase allele capture. Increase in allele capture with increasing sample size is greatest at low resample sizes, and showed diminishing returns as resample size increased. No appreciable increase in allele capture was gained through maintaining different half-sibling groups. These data inform sampling for ex situ conservation purposes, and recommend sample sizes of at least 15 individuals, with the upper limit based on resources.     

Effect of lithological substrate on microbial biomass and enzyme activity in brown soil profiles in the northern Apennines (Italy)

The aim of the present study was to investigate the microbial activity along forest brown soil profiles sequence developed on different lithological substrates (carbonate or non-carbonated cement in sandstone formations) at different altitudes. The main question posed was: does carbonate affect the biochemical activity of brown soil profiles at different altitudes? For the purpose of this study, four soil profiles with different amounts and compositions of SOM developed on different lithological substrates were selected: two with carbonate (MB and MZ) and the other two with non-carbonated cement in the sandstone formations (MF1 and MF2). Chemical and biochemical properties of soil were analysed along soil profiles in order to assess the SOM quantity and quality, namely total organic C (C_org), water extractable organic C (WEOC) and humification indices (HI, DH, HR). Microbial biomass (C_mic and N_mic) content, as well as the specific activities of acid phosphatase, β-glucosidase and chitinase enzymes were chosen as indicators of biochemical activity. The soil biochemical properties provided evidence of better conditions for microorganisms in MB than in MF1, MF2 and MZ soil profiles, since patterns of microbial biomass content and activity might be expected in response to the amount and quality of organic substances. The different lithological substrates did not show any clear effect on soil microbial biomass content, since similar values were obtained in MF1, MF2 (with non-carbonated cement) and MZ (with carbonate). However, the specific activities of acid phosphatase (per unit of C_org and per unit of C_mic) were higher in soils with no carbonate (MF1 and MF2) than in soils with carbonate (MB and MZ). In conclusion, the biochemical activity along brown soil profiles was mainly regulated by different soil organic matter content and quality, while the two different lithological substrates (with carbonate or non-carbonated cement in the sandstone formations) did not show any direct effect on microbial biomass and its activity. However, the activity of acid phosphatase per unit of C was particularly enhanced in soil with non-carbonate cement in the sandstone formations.     

Nitrogen isotopic composition of leached nitrate and soil organic matter as an indicator of denitrification in a sloping drained agricultural plot and adjacent uncultivated riparian buffer strips

In the small, agricultural, artificially drained Orgeval watershed δ¹⁵N values of leached nitrates and soil organic nitrogen were found to be significantly higher than the primary nitrogen (N) sources from which they are derived, namely, synthetic fertilizers, atmospheric deposition, and symbiotic or nonsymbiotic N₂ fixation (all with δ¹⁵N close to zero). In vertical soil profiles, the δ¹⁵N of organic N increased with depth, reaching higher values (up to 8‰) particularly at stations that were frequently waterlogged as judged from ochre iron traces, such as downhill field sites or in riparian buffer strips. Nitrification, volatilization, and denitrification are the main fractionating processes able to modify the isotopic composition of soil N. Using a newly designed algorithm for calculating the equilibrium isotopic composition of all soil N species, resulting from the average annual balance of their transformations, we show that the observed trends can be explained by the action of denitrification. We suggest that the isotopic composition of soil organic N can be used as a semiquantitative indicator of the intensity of denitrification integrated over century-long periods.     

Microbial respiration activities related to sequentially separated, particulate and water-soluble organic matter fractions from arable and forest topsoils

This study aimed to reveal differences in the relevance of particulate as well as water-soluble organic matter (OM) fractions from topsoils to the easily biodegradable soil organic matter (SOM). We selected eight paired sites with quite different soil types and soil properties. For each of these sites, we took samples from adjacent arable and forest topsoils. Physically uncomplexed, macro-, and micro-aggregate-occluded organic particle, as well as water-soluble OM fractions were sequentially separated by a combination of electrostatic attraction, ultrasonic treatment, density separation, sieving, and water extraction. The easily biodegradable SOM of the topsoil samples was determined by measuring microbial respiration during a short-term incubation experiment (OC_R). The organic carbon (OC) contents separated by i) the physically uncomplexed water-soluble OM, ii) the macro-, and iii) the micro-aggregate-occluded organic particle as well as water-soluble OM fractions were significantly correlated with OC_R. The correlation coefficients vary between 0.54 and 0.65 suggesting differences in the relevance of these OM fractions to the easily biodegradable SOM. The strongest correlation to OC_R was detected for the OC content separated by the physically uncomplexed water-soluble OM indicating the most distinct relation to the easily biodegradable SOM. This was found to be independent from land use or soil properties.