猪源唾液乳杆菌对生长猪氮表观消化率及血清指标的影响

本研究旨在探讨猪源唾液乳杆菌对生长猪氮表观消化率和血清指标的影响。选用平均体重为(35.22±0.86)kg长×大二元杂交猪81头,随机分成3组,每组3个重复,每个重复9头。对照组饲喂基础日粮,试验组分别在基础日粮中添加0.20%、0.40%猪源唾液乳杆菌冻干制剂,试验期为30 d。结果表明:添加0.20%、0.40%的猪源唾液乳杆菌冻干制剂可显著提高生长猪氮表观消化率(P<0.05);与对照组相比,2个试验组猪血清白球比显著降低(P<0.05),尿素氮、乳酸脱氢酶、肌酸激酶含量分别比对照组降低了14.77%和13.42%(P<0.05)、16.92%和20.0%(P<0.05)、28.96%和23.00%(P<0.01),但2个试验组间差异不显著(P>0.05)。结果提示,日粮中添加猪源唾液乳杆菌能够提高生长猪氮表观消化率,改善血清指标,减少应激,且以添加剂量为0.20%时效果最好。     

玉米饲用营养价值的氮素调控

采用盆栽法研究了施氮时期对玉米鲁单50饲用营养价值的调控作用。结果表明,施氮肥处理玉米的生物产量、籽粒产量以及饲用营养品质都显著的高于不施氮肥的。拔节期一次性施用氮肥可以获得较高的生物产量,有利于提高玉米作为青饲（贮）饲料的营养价值;拔节、大口（或开花）期,2或3次施氮肥有利于提高粮饲兼用玉米的饲用营养价值。     

施氮肥对高羊茅种子质量和产量组成的影响

2000-2001年在新疆塔里木农垦大学牧草试验站进行了施氮肥对交战高羊茅种子质量和产量组成影响的研究。结果表明,施氮可以明显提高种子的活力和质量,并增加了单位面积的生殖枝数和小花数／小穗;施氮肥提高了种子产量,其中以秋季60kg／hm^2 春季120kg／hm^2施氮量为最好,种子产量可达1653．9kg／hm^2。     

0~4周龄云岭红肉鸡赖氨酸适宜需要量的研究

试验通过分析饲粮不同赖氨酸水平对云岭红肉鸡生长性能、氮沉积率及血液生化指标的影响,确定云岭红肉鸡饲粮赖氨酸的适宜需要量。采用单因素试验设计,将180只健康状况良好、体重相近的1日龄云岭红肉鸡随机分为5个处理组,每组3个重复,每个重复12只鸡。5组试验鸡饲喂的饲粮赖氨酸水平分别为0.85%、0.95%、1.05%、1.15%、1.25%。结果表明:赖氨酸水平对试验鸡的期末体重、全期增重、平均日增重及料重比有显著影响(P<0.05),当赖氨酸水平为1.15%时期末体重最大739.21 g,全期增重最大701.66 g,平均日增重最大24.19 g,料重比最低1.68。不同赖氨酸水平对丝氨酸、甘氨酸和组氨酸的表观代谢率影响显著(P<0.05)。对血清总氨基酸和血清尿素氮影响显著(P<0.05)。研究表明,0~4周龄云岭红肉鸡适宜赖氨酸水平最适量为1.15%。     

饲用玉米根际促生菌资源筛选及其特性研究

为获得玉米根际促生菌(PGPR)菌株并明确其功能特性;以玉米根系及根际土壤为材料,利用选择性培养基分离筛选溶磷菌与固氮菌,测定所选菌株溶磷能力和固氮酶活性,并测定其产IAA的能力,从中筛选综合性能优良的菌株,在此基础上,运用生理生化鉴定和16SrDNA分子生物学鉴定相结合的方法鉴定优良菌株的种属。结果表明:玉米根际有大量PGPR菌,分布特征表现出根表(RP)根表土(RS)远根土(NRS)根内(HP)的数量分布规律,呈根际效应。最终获得262株PGPR菌,其中固氮菌48株,溶解无机磷细菌108株,溶解有机磷细菌106株,有分泌IAA能力的菌株15株。筛选出综合性能优良、有进一步开发应用潜力的菌株7株。经鉴定其中3株为Pseudomonas fluorescens;2株为Bacillus subtilis;Klebsiella oxytoca和Bacillus megaterium各1株。     

养殖环境生物除氮脱氮技术研究进展

随着我国水产养殖业的快速发展,养殖环境中氮的污染问题日益严重。生物除氮脱氮技术由于具有无可比拟的优势而受到人们的重视,近年来无论在基础理论还是应用实践方面发展迅速。本文总结了水产养殖中该项技术的最新研究进展,并指出今后的研究方向和重点。     

氮素形态对紫花苜蓿盛花期生长及结瘤固氮的影响

在完全营养液条件下,采用砂培法,研究了3种氮素形态配比NO3--N,NH4+-N和NO3--N∶NH4+-N为1∶1和0、105、210mg/L 3个氮素水平对紫花苜蓿盛花期生长及结瘤固氮的影响。结果表明:不同形态氮素处理下紫花苜蓿的株高、生物量、根瘤数、根瘤重、固氮酶活性和全氮含量均显著高于CK。同一氮素水平下,株高表现为NO3--N和NH4+-N混合培养下效果最好,NO3--N培养下次之,NH4+-N培养下最低;生物量、根瘤数、根瘤重、固氮酶活性和全氮含量NO3--N和NH4+-N混合培养下效果最好,NH4+-N培养下次之,NO3--N培养下最低;茎叶比则是NO3--N培养下最大,NH4+-N培养下次之,NO3--N和NH4+-N混合培养下的最低,各氮素形态处理间差异显著(P0.05)。随着氮素水平的增加,各形态配比下紫花苜蓿的株高、生物量、根瘤数、根瘤重、固氮酶活性和全氮含量呈现增加的变化趋势,茎叶比呈现减小的变化趋势。NO3--N+NH4+-N的浓度为210mg/L时,紫花苜蓿的株高、生物量、根瘤数、固氮酶活性和全氮含量取得最大值,茎叶比取得最小值,根瘤重则在NO3--N+NH4+-N的浓度为105mg/L时取得最大值,紫花苜蓿在混合态氮培养下生长最好。     

Determination of critical nitrogen dilution curve based on leaf area index for winter wheat in the Guanzhong Plain,Northwest China

Excessive use of nitrogen(N) fertilizers in agricultural systems increases the cost of production and risk of environmental pollution. Therefore, determination of optimum N requirements for plant growth is necessary. Previous studies mostly established critical N dilution curves based on aboveground dry matter(DM) or leaf dry matter(LDM) and stem dry matter(SDM), to diagnose the N nutrition status of the whole plant. As these methods are time consuming, we investigated the more rapidly determined leaf area index(LAI) method to establish the critical nitrogen(N_c) dilution curve, and the curve was used to diagnose plant N status for winter wheat in Guanzhong Plain in Northwest China. Field experiments were conducted using four N fertilization levels(0, 105, 210 and 315 kg ha-1) applied to six wheat cultivars in the 2013–2014 and 2014–2015 growing seasons. LAI, DM, plant N concentration(PNC) and grain yield were determined. Data points from four cultivars were used for establishing the N_c curve and data points from the remaining two cultivars were used for validating the curve. The N_c dilution curve was validated for N-limiting and non-N-limiting growth conditions and there was good agreement between estimated and observed values. The N nutrition index(NNI) ranged from 0.41 to 1.25 and the accumulated plant N deficit(N_(and)) ranged from 60.38 to –17.92 kg ha~(-1) during the growing season. The relative grain yield was significantly affected by NNI and was adequately described with a parabolic function. The N_c curve based on LAI can be adopted as an alternative and more rapid approach to diagnose plant N status to support N fertilization decisions during the vegetative growth of winter wheat in Guanzhong Plain in Northwest China.     

Overstory vegetation influence nitrogen and dissolved organic carbon flux from the atmosphere to the forest floor: Boreal Plain,Canada

Nitrate, ammonium, total dissolved nitrogen (TDN), dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) concentrations and flux were measured for one year in bulk deposition and throughfall from three stand types (upland deciduous, upland conifer and wetland conifer) on the Boreal Plain, Canada. Annual (November 2006 to October 2007 water year) flux rates in bulk deposition were 80, 216, 114 and 410 mg N m⁻² for nitrate, ammonium, DON and TDN, respectively, and 3.5 g C m⁻² for DOC. The nitrate and ammonium flux in throughfall were approximately 50% of the flux in bulk deposition, while TDN flux in throughfall was 60–74% of the flux in bulk deposition. The DOC flux in throughfall was approximately 2 times greater than DOC flux in bulk deposition, while there was no detectable difference in DON flux. The forest canopy generally had the most impact on throughfall chemistry during the active growing season as compared with the dormant season, although DOC concentrations in throughfall of deciduous stands was highest during autumn. For the upland stands, TDN flow-weighted mean concentrations in the snowpack were not detectably different from the concentrations in throughfall and bulk deposition throughout the rest of the year. However, ammonium concentrations were lower and DON concentrations were higher in the snowpack than in either throughfall or bulk deposition for the other seasons, suggesting some transformation of ammonium to DON within the snowpack.     

水、氮效应与叶绿素关系试验研究

以冬小麦和夏玉米为试验对象，研究了不同水、氮条件对作物植株含氮及叶绿素含量的影响，作物叶片含氮、作物产量与叶绿素含量之间的量化关系，并提出了利用叶绿素仪预测作物氮素胁迫的方法。研究结果表明，田间的不同水、氮制度将影响作物的含氮水平及叶绿素含量，它们之间呈正响应关系。氮素对作物叶绿素含量起着较强的影响作用。作为一种测量叶片叶绿素含量的仪器，叶绿素仪可以作为测量作物含氮的仪器使用，具有快速、准确、简便的特点。