Effect of captopril on AGS nude mouse model of gastric cancer

AIM: To observe the effect of captopril on the genesis and development of gastric cancer, and to explore its clinical treatment feasibility for gastric cancer. METHODS: The human gastric cancer cell line AGS was used to establish a tumor model in nude mice, and the model mice were randomly divided into 3 groups: positive control (5-fluorouracil) group, normal control (saline) group and experimental (captopril) group. After intraperitoneal injection or intragastric administration of the drugs, the tumor growth curve was determined, and the tumor tissues were also sampled to detect the expression of Ki-67, STAT3, Bax and Bcl-2 by real-time quantitative PCR and immunohistochemistry. The apoptosis was detected by TUNEL+DAPI staining. RESULTS: The tumor growth curve showed that the tumor model in the nude mice was successfully established. The tumor volumes among groups showed significantly different after 14 d growth. The increase in the tumor volume in normal control group was significantly faster than that in the other two groups, and that in positive control group was the slowest. The expression of Bax in captopril group increased, and the expression of STAT3, Ki-67 and Bcl-2 was reduced as compared with normal control group and positive control group. Compared with normal control group, the apoptotic rate increased significantly, and the protein expression of p-STAT3 and STAT3 decreased obviously in positive control group and captopril group. CONCLUSION: With better feasibility, angiotensin-converting enzyme inhibitor captopril has a significant effect on treating gastric cancer in the AGS nude mouse model by regulating the expression of STAT3, Bax, Bcl-2 and Ki-67 to accelerate the apoptosis of cancer cells, thus inhibiting tumor growth.     

Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice

Activation of the innate immune system requires recognition of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro-inflammatory cytokine, IL-1β, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we investigated the tissue distribution and expression pattern of NLRP3 in BALB/c mice to further elucidate its function in innate immunity in this commonly used laboratory animal model. NLRP3 mRNA expression levels and tissue distribution of the protein were investigated by real-time quantitative PCR and immunohistochemical analyses, respectively. NLRP3 mRNA expression was higher in the kidney and inguinal lymph nodes than in other tissues. Cytoplasmic expression of NLRP3 was detected in the epithelial reticular cells of the spleen and thymus, lymphocytes in the inguinal lymph nodes, cardiac muscle cells, cerebral cortex neurons, alveolar macrophages, renal tubule cells and liver sinusoidal endothelial cells. The results of this study will assist investigators in interpreting site-specific functions and roles of NLRP3 in inflammatory responses.     

乳酸菌―芽孢菌发酵黄芪对小鼠免疫功能作用的研究简

试验旨在探讨乳酸菌―芽孢菌发酵黄芪对小鼠免疫功能的影响。将84只小鼠分成6组,每组14只,分别为生理盐水组（阴性对照组）、未发酵黄芪组（阳性对照组,黄芪生药浓度为150 mg/mL）、试验Ⅰ组（黄芪生药浓度为60 mg/mL）、试验Ⅱ组（黄芪生药浓度为90 mg/mL）、试验Ⅲ组（黄芪生药浓度为120 mg/mL）、试验Ⅳ组（黄芪生药浓度为150 mg/mL）。小鼠采用灌胃方式给药30 d,最后处死小鼠测定其体重、免疫器官（胸腺和脾脏）系数、免疫因子（IgG、IL-6、sIgA）。结果显示,给药30 d后,试验组小鼠体重明显升高;试验Ⅰ组免疫器官系数极显著高于对照组（P＜0.01）;试验Ⅲ、Ⅳ组免疫因子IgG和sIgA均极显著高于对照组（P＜0.01）。由此可知,乳酸菌―芽孢菌发酵黄芪对小鼠的免疫功能有很好的促进作用。     

5年生种植人参对正常成年小鼠睡眠的影响

目的观察5年生种植人参食用对正常成年小鼠睡眠的影响,为人参的食用提供实验依据。方法正常成年昆明小鼠140只,雌雄各半,体重18-22g,随机分为10组,即:对照组,5年生种植人参原粉小、中、大剂量(0.25、0.5、1.0g生药/kg)组,5年生种植人参水提物小、中、大剂量(0.25、0.5、1.0g生药/kg)组,5年生种植人参醇提物小、中、大剂量(0.25、0.5、1.0g生药/kg)组,每组14只。各组小鼠连续灌胃给药3个月后进行戊巴比妥钠催眠、戊巴比妥钠阈下催眠及水合氯醛阈下催眠实验。结果戊巴比妥钠催眠实验中,与对照组比较,人参原粉小、中、大剂量组,人参水提物小、中、大剂量组及人参醇提物小、中、大剂量组睡眠的潜伏期及睡眠持续时间无明显变化(P0.05);戊巴比妥钠阈下催眠,水合氯醛阈下催眠实验中,与对照组比较,人参原粉小、中、大剂量组,人参水提物小、中、大剂量组及人参醇提物小、中、大剂量组30min内睡眠发生率无明显变化(P0.05)。结论 5年生种植人参食用对正常成年小鼠睡眠无明显影响。     

中药复方剂对小鼠抗应激作用的研究

以小鼠为研究对象,通过持续给药和应激处理,观察试验组与对照组在不同阶段小鼠体重和采食量等的变化。试验期为15d,试验结束后处死小鼠,称量体重和饲料消耗量,分析体增重、日增重和饲料转化率。结果表明:应激时,动物的体增重和饲料转化率等均不高,但服用中药复方剂的试验组反应明显要比对照组小。此为中药复方剂在临床生产中作为抗应激剂提供一定的理论指导和依据。     

保胎无忧散对小鼠诱导性流产的保胎作用

选择保胎无忧散预处理试验小鼠,观察保胎无忧散对米非司酮(RU486)诱导流产作用的抑制效果.结果显示,使用RU486在孕7d,颈部注射后,试验小鼠的流产率达到了100％；应用中药方剂保胎无忧散能有效对抗RU486诱导的小鼠流产,试验中动物模型的流产率从100％下降到40％；组织学观察发现,RU486诱导流产小鼠子宫内膜出现明显损伤,而在中药方剂组,试验小鼠子宫得到了有效的保护.     

不同抗热应激添加剂对热应激小鼠精液品质的影响

为探讨抗热应激添加剂对热应激状态下小鼠精液品质的影响,选取年龄和健康状况相近的成年雄性鼠150只,按照常温、热应激、添加剂处理后热应激的方式进行分组试验,对各组精液品质等指标进行统计分析。结果表明,3组热应激添加剂在缓解热应激小鼠的精液品质的影响效果上存在显著差异,其中效果最好的为中药组,其次是电解质组,最后是维生素组。从Hsp70mRNA表达量上来看,抗热应激添加剂组明显高于常温对照组。但与热应激组比较,维生素组与电解质组表达量低,而中药组表达量高。     

抗球虫中药散剂常青散的亚急性毒性试验

为了测定抗球虫中药散剂常青散的亚急性毒性反应,取昆明小鼠为试验动物,随机分组,以临床应用剂量的2、4、8倍饲喂给药,确定常青散在28天内对小鼠的亚急性毒性反应。结果表明各试验组小鼠全部健活,体征、体重、外观、行为、采食量及饲料利用率、脏器系数、血液生理生化指标、脏器结构均无异常。常青散对受试动物未见有明显的亚急性毒性反应。     

壳聚糖硒对小鼠的抗氧化作用研究

选择150只25g左右的健康昆明种小白鼠,随机分为6组。1组为对照组,饲喂低硒基础日粮;2组为壳聚糖组;3组为亚硒酸钠组,在基础日粮中以亚硒酸钠的形式添加硒0.25mg/kg;4~6组为壳聚糖硒组,分别在基础日粮中以壳聚糖硒的形式添加硒0.125mg/kg、0.25mg/kg、0.5mg/kg,研究壳聚糖硒对小鼠抗氧化作用的影响。结果显示,6组血浆中谷胱甘肽过氧化物酶(GSH-Px)活性显著高于5组(P<0.05),极显著高于1组、2组(P<0.01);6组血浆中超氧化物歧化酶(SOD)活性极显著高于1组、2组(P<0.01);1组、2组血浆中过氧化氢(H2O2)、丙二醛(MDA)的含量显著高于3组、4组(P<0.05),极显著高于5组、6组(P<0.01)。试验表明,日粮中添加不同水平壳聚糖硒能够使小白鼠血浆中GSH-Px、SOD的活性显著提高,使小白鼠血浆中的MDA、H2O2的含量显著降低,进而提高小白鼠的抗氧化能力。     

中药"壮阳促孕散"对小白鼠传统致畸试验的研究

用昆明种小白鼠对中药“壮阳促孕散”进行传统致畸试验研究。在试验中，对所有孕鼠的着床数、活胎数、死胎数、吸收胎数、胎鼠体重、身长、尾长、颜色、形状、大小、数量以及胎鼠的内脏和骨骼进行了检查记录。结果表明：致畸反应呈阴性，这说明中药“壮阳促孕散”无致畸作用。