LIF与心肌条件液在小鼠ES细胞分离中的差异比较

通过比较LIF和大鼠心肌条件液在小鼠ES细胞分离培养过程中的差异, 从而选择较为合适的培养液用于小鼠ES细胞的分离培养与深入研究。取怀孕3.5 d小鼠囊胚, 培养于小鼠胎儿成纤维细胞饲养层上, 然后根据ES细胞培养液的不同分成2组,一组添加LIF的ES细胞培养液,另一组添加由大鼠心肌条件液组成的ES细胞培养液。结果显示,小鼠ICM的孵出率在心肌条件液中为76.30 %, LIF条件液中为59.35 %,两者差异极显著(p＜0.01)))))) ; 在LIF条件液中比心肌条件液中能较早地分离出ICM,时间差分别为11、11、12、10和12 h,平均为11.2 h; 对传代的ES细胞集落,培养48 h时周边出现分化现象的ES细胞集落所占的比例在心肌条件液中为51.55%, LIF条件液中为31.69 %,两者差异显著(p＜0.05); 第五代小鼠ES细胞核型正常率在心肌细胞条件液中为78.6 %, 稍高于LIF条件液中的76 %,差异不显著。     

干燥机粮层通风阻力特性数值模拟与试验

粮层内部通风阻力特性是影响干燥均匀性和能量损耗的关键因素之一。针对传统的经验公式存在精度偏低、未考虑粮层多孔介质的复杂变化因素和非线性的不确定性问题,基于传统的Ergun模型进行了压力损失因素分析;在通风阻力试验平台测定试验数据,通过探索性分析方法对风速进行分段,引入误差影响因子λ,导出了一种新的基于多孔介质Ergun的稻谷变层压力场模型;给出了优化阻力特性曲线,构建了变层阻力与层厚的数学关系。试验结果证实,风速小于0.2m/s时,λ取值为1;风速在0.2~0.4m/s范围内时,λ取值为0.89;风速在0.4~0.6m/s范围内时,λ取值为0.79。     

不完全分形多孔介质热导率模型

将多孔介质的物理构成分为具有分形结构的团聚体集合和不具有分形特性的固相和孔隙相,建立了简化单元体模型解释其微观结构。结合多孔介质在干燥过程中热量守恒定律和傅里叶导热定律导出了材料总有效热导率模型。此模型无经验常数,每一个参数都有物理意义。研究结果表明,有效热导率与迂曲分形维数、面积分形维数、孔隙率和热风温度呈反比,与热风速率和时间呈正比。     

滚珠丝杠进给系统动态特性集中质量建模与仿真

为了研究高速滚珠丝杠进给系统的动态特性,提出一种改进的集中质量建模方法,该方法考虑了滚珠丝杠进给系统基座的质量、柔性及进给系统部件间反向间隙对系统动态特性的影响,并完善了滚珠丝杠进给系统集中质量模型等效参数的计算方法。以MAG某加工中心X轴滚珠丝杠进给系统为实验对象,对该集中质量模型建模方法进行实验验证,仿真结果与实测结果具有相同数量的特征频率,且特征频率的误差在5%以内。滚珠丝杠进给系统的仿真分析表明,螺母位置改变引起的进给系统固有频率变化将影响伺服系统的稳定性,滚珠丝杠进给系统中的反向间隙会激励部件产生高频振动,给进给系统带来不利影响。     

Influence of long-term fertilization with farmyard manure on soil organic matter: Characteristics of particle-size fractions

Summary The influence of more than 100 years of fertilization with farmyard manure on soil organic matter in comparison to unfertilized soil was studied in particle-size fractions using elemental (C and N) analyses and pyrolysis-field ionization mass spectrometry. Distinct differences in C and N concentrations and distribution and in the quality of organic matter between the size fractions and the fertilization treatments were observed. Clay-associated C and N were relatively higher in the unfertilized treatment, whereas the application of farmyard manure preferentially increased soil organic matter associated with the fine and medium silt fractions. Pyrolysis-field ionization mass spectrometry of soil fractions <20 m showed increasing values for lignin monomers and dimers and fatty acids with larger equivalent diameters, whereas the proportion of N compounds, mono- and polysaccharides and phenolics decreased in the larger size fractions. Sand fractions were particularly rich in lignin fragments, mono- and polysaccharides, and alkanes/alkenes. These relationships seemed to be independent of management practices. In the same size fractions of the different treatments, however, a higher relative abundance of N-compounds, mono- and polysaccharides, phenolics, lignin monomers, and alkanes/alkenes was observed in the unfertilized variant. Lignin dimers and fatty acids were more abundant in the farmyard manure treatment. Both trends together imply that soil enrichment in organic matter due to the application of farmyard manure largely reflects an increase in lignin building blocks and partly reflects an increase in lipids such as fatty acids in the silt fractions. Therefore these constituents are of particular importance in assessing the positive effects of farmyard manure on soil fertility.     

球形食品真空冷却过程中参数分析

建立了描述球形食品在真空冷却中传热、传质的数学模型。通过数值求解得到真空压力、产品质量、产品温度(表面温度、中心温度、质量平均温度)随时间的变化曲线。实验装置中高精度的数据采集系统能够在线测定和记录真空室压力、产品质量、产品不同位置的温度随时间的变化。在春收甘蓝的真空冷却实验中，模拟的压力和实测的压力在10000～600 Pa的降压过程中最大相差100 Pa；模拟值和实测值间温度的最大差值小于1℃；真空冷却中，模拟的甘蓝的水分损失率是3.32%，实测的甘蓝水分损失率是2.97%，相差0.35%。研究结果对     

平行梁冲量式谷物质量流量传感器田间实验

设计了平行梁冲量式谷物质量流量传感器,以该传感器为核心部件构建了测产系统。针对小麦、水稻两种不同谷物品种,不同联合收获机机型以及不同田间地面状况,于2004年夏秋两季分别在中国科学院栾城农业生态系统实验站和上海五四农场做了精准收获实验。实验结果表明,测产系统可以在正常农田地面状况下可靠地工作,单产和总产测量误差均小于10%,且对联合收获机机型以及谷物品种不敏感。     

多孔介质水分运动的随机数值分析

采用van Genuchten-Mualem模型描述非饱和水力传导度和水分特征曲线,将模型中的3个参数作为随机场处理,联合运用Karhunen-Loeve展开和摄动方法,得到饱和非饱和水流的一个随机数值模型。应用该模型分析了介质随机参数的空间变异性对水头分布的影响,结果表明,水头标准差对介质参数的敏感程度由大到小依次为非饱和参数n、β、饱和水力传导度Ks,饱和带水头分布的变异性较非饱和带小。     

菇渣和锯末作为丽格海棠栽培基质的研究

为减少花卉无土栽培对泥炭资源的依赖,同时降低生产成本,该研究以菇渣和锯末两种农林有机废弃物作为基质的主要成分栽培丽格海棠,探讨取代泥炭作为丽格海棠栽培基质的可行性。结果表明,4锯末:1珍珠岩和3锯末:1蛭石两个处理的理化性质的各项指标均在无土栽培基质的理想范围内,用其栽培的丽格海棠根系生长健壮,生长发育综合表现显著优于对照,两个处理的成本分别比对照降低27.61%和27.23%,可作为丽格海棠无土栽培的代用基质。     

Using composted wheat residue as a growth medium in culture of tomato

A greenhouse experiment was carried out to investigate the effects of different growing media on some properties of tomato (Lycopersicom esculentum L. cv. Falcato). Four growing media including wheat residue, composted wheat residue, coco peat + perlite, and coco peat + soil were applied in pot experiment with completely randomized design with three replications. The results showed that fruit yield, chlorophyll, and vitamin C contents of tomatoes grown in the composted wheat residue and coco peat + perlite treatments were significantly greater than the other growth media. Fresh weight of root and inflorescence were higher in the tomatoes cultivated in the composted wheat residue pots than the other media. Higher internode length and nutrient concentrations in leaf and fruit at the coco peat + perlite treatment were obtained as compared to the other growth media treatments. According to the results, it can be concluded that the coco peat + perlite can be substituted for by composted wheat residue for the cultivation of tomato.