MEASUREMENT OF BODY COMPOSITION IN CATS USING COMPUTED TOMOGRAPHY AND DUAL ENERGY X‐RAY ABSORPTIOMETRY

Dual energy X‐ray absorptiometry (DEXA) is a reference method for assessing body composition but is seldom `accessible in veterinary settings. Computed tomography (CT) can provide similar body composition estimates and we propose that it can be used in body composition studies in animals. We compared CT and DEXA data from 73 healthy adult neutered domestic cats. Three approaches for measuring adipose tissue percentage from full‐body CT scans were explored. By examining the frequency distribution of voxels by Hounsfield unit (HU) value, it is possible to calculate a fat index (Fat%) that is in close agreement with the fat percentages obtained from DEXA scans. Fat% values obtained by the best of the methods had a mean difference of 0.96% (95% confidence interval 0.33–1.59%) from the DEXA results. Fat% obtained by the other two methods were characterized by good correlation but poor agreement and in one of the methods, the difference between the values from the two modalities was proportional to their mean. By using CT, it is possible to obtain body composition estimates that are in close agreement with those available using DEXA. While the significance of individual Fat% measurements obtained from CT can be difficult to interpret and to compare between centers, CT can contribute to research studies concerned either with nutrition or with obesity‐related disorders.     

高效液相色谱-蒸发光散射检测法测定板蓝根中精氨酸的含量

为了建立中药材板蓝根中精氨酸的含量测定方法,采用色谱柱为ZORBAXSB-Aq(5μm,4.6×250mm),柱温30℃,以水-三氟醋酸(100∶0.2)为流动相,流速0.3mL/min;蒸发光散射检测器检测,漂移管温度110℃,载气流量2.5L/min。结果显示,线性方程为Logm=0.62630·LogA-2.29056,相关系数R=0.9996,表明精氨酸在0.2μg^1.6μg范围内线性关系良好。平均加样回收率为100.5%,RSD=1.4%(n=9)。表明该方法简便准确,重复性好。     

青海半细毛羊羊毛纤维长度与含硫量的分布

通过对青海半细毛羊羊毛纤维长度方向1cm区段含硫量的测定。结果表明青海半细毛羊纤维不同长度区段的含硫量不同，含硫顶峰值公羊1岁9cm，2岁14cm，成年14cm，母羊1岁12cm，2岁8cm，成年10cm。毛纤维垂直方向上，中，下三段中，含硫量以中段最高。二岁公羊毛长与含硫量的正相关，相关系数R＝0．75735，一岁母羊呈中等正相关，R＝0．31762，其余各性别年龄组内呈弱的负相关，相关系数为R＝－0．01016－0．23774。     

36个燕麦品种不同部位养分分布格局

分析了天祝高寒草甸区36种不同饲用燕麦(Avena sativa)叶片、籽粒和茎秆中的水分、灰分、粗蛋白、可溶性糖、粗脂肪、磷、钙、粗纤维、中性纤维和酸性纤维含量的分布特征。结果显示:36个燕麦品种叶片中水分、灰分、粗蛋白、可溶性糖和钙含量显著高于籽粒和茎秆(P0.05),平均值分别为6.36%、9.28%、19.82%、13.71%和0.25%;籽粒中粗脂肪和磷含量显著高于叶片和茎秆(P0.05),平均值分别为:4.48%和0.25%;茎秆中粗纤维、中性洗涤纤维和酸性洗涤纤维含量显著高于叶片和种子(P0.05),平均值分别为35.62%、65.18%和56.40%。总之,36种燕麦不同部位的营养价值高低依次为叶片籽粒茎秆。     

7种能量饲料淀粉降解率的研究

以6头装有永久性瘤胃瘘管的浏阳去势黑山羊为实验动物,利用瘤胃尼龙袋技术研究大麦、大米、高粱、荞麦、小麦、小米、玉米等7种常用谷物饲料淀粉在羊瘤胃内的降解率规律。结果表明各种饲料淀粉有效降解率(%)顺序为:大麦>小麦>玉米>大米>荞麦>高粱>小米。其中以大麦的淀粉有效降解率最高达97.05%,小米的最小为36.43%,其它依次为96.28%、55.37%、45.23%、44.10%、42.58%。     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.     

营养水平对仔猪断奶综合征的影响

试验选取杜×长×大三元杂交仔猪,随机分为2个处理组,每个处理3个重复,每个重复10头仔猪.试验组饲喂5008配合日粮.对照组饲喂5000配合日粮.针对仔猪断奶综合征的表现特征,分别测定各组猪生长性能和腹泻率等指标.试验结果表明,饲粮中合有高水平的蛋白质及能量对断奶仔猪有促生长作用,可缓解仔猪断奶综合征的发生.     

不同品种类型玉米不同器官和全株生物产量比较

本试验旨在比较不同品种类型玉米(Zea mays)不同器官和全株的生物产量,以提高玉米定向培育的选择效率。选择32种具有代表性的玉米优良品种为试验材料,根据淀粉性质、籽实外形、成熟性质和用途4种分类方法,分别对完熟期玉米的籽实、玉米芯、苞叶、叶片、叶鞘、茎秆及全株的鲜物质、风干物质、干物质以及粗蛋白质和总能产量进行比较。每个品种选择5个地块,每个地块选取10株玉米。结果表明:玉米的籽实、玉米芯、苞叶、叶片、叶鞘、茎秆和全株的鲜物质、风干物质、干物质、粗蛋白质和总能产量不同品种类型间差异均显著(P0.05)。根据淀粉性质、籽实外形和成熟性质分类不足以区别不同品种类型玉米的不同器官和全株生物产量(P0.05)。按用途分类,不同品种类型玉米不同器官和全株生物产量(玉米籽实的风干物质、干物质和总能产量除外)均差异显著(P0.05),表现为饲用玉米红粒玉米普通玉米糯玉米。对广泛种植的普通玉米和饲用玉米进行比较,设定普通玉米产量为100.0%,则饲用玉米籽实、玉米芯、苞叶、叶片、叶鞘、茎秆和全株的鲜物质产量分别为121.9%、164.2%、175.5%、149.0%、151.7%、168.8%和149.9%;风干物质产量分别为107.9%、143.7%、155.1%、139.4%、126.1%、162.0%和129.1%;干物质产量分别为107.5%、143.4%、155.0%、139.5%、125.7%、162.0%和129.21%;粗蛋白质产量分别为119.0%、142.9%、181.5%、161.6%、122.0%、137.4%和131.7%;总能产量分别为107.7%、143.8%、155.0%、139.4%、126.1%、162.0%和129.1%。玉米籽实干物质、粗蛋白质和总能产量占玉米全株的比例,普通玉米分别为47.75%、57.87%和48.49%,饲用玉米分别为39.72%、52.36%和40.48%。由此得出,普通玉米全株饲喂是"粮改饲"的最基本的策略,而饲用玉米籽实和全株生物产量都高于普通玉米,种植饲用玉米可以增加30%左右的土地利用。     

基于线性谱聚类的林地图像中枯死树监测

【目的】将基于线性谱聚类超像素的方法应用在森林病虫害防治领域,可智能监测无人机森林虫害图像中的枯死树,为森林有害生物的监测工作提供技术支撑。【方法】分别以湖北省受松材线虫与辽宁省受红脂大小蠹侵害的松林无人机图像为试验数据,首先使用线性谱聚类超像素分割算法将图像划分为多个超像素;然后基于枯死树木的颜色特征,初步提取可能为枯死树的超像素区域;最后基于枯死树木与其他干扰地物具有不同的纹理特征,计算超像素的区域密度和缝隙量,利用支持向量机对初步提取的超像素进行分类,从而检测出图像中的枯死树。【结果】基于线性谱聚类超像素和支持向量机的枯死树监测方法可有效排除与枯死树木颜色相近的其他干扰地物,较准确地提取出枯死树木。使用该方法与基于植被颜色指数的阈值分割方法、基于简单线性迭代聚类超像素和随机森林的方法,对35幅受灾松林无人机图像进行试验,并选用交并比、虚警率和漏检率3个评价指标对3种方法进行定量对比分析。结果表明,基于线性谱聚类超像素的方法监测出的枯死树区域最精确,其监测结果与人工检测结果的交并比均值大于58%,且虚警率和漏检率均优于另外2种方法。【结论】基于线性谱聚类超像素的枯死树监测方法能实现松林中枯死树的快速、准确检测及定位。