芽孢杆菌绿色荧光蛋白标记及其在小麦体表定殖的初探

 将来自质粒pAD4412的启动子和绿色荧光蛋白基因gfpmut3a插入大肠杆菌-枯草芽孢杆菌穿梭载体pBE2,构建成芽孢杆菌表达载体pGF P4412,用其转化野生型生防芽孢杆菌83-6和A-47等8个菌株,均得到良好的发光表型。质粒稳定性实验表明重组质粒pG FP4412稳定性为92%。借助荧光显微镜对gfp标记的菌株A-47-gfp在小麦体表的定殖进行初步的研究。结果表明:A-47-gfp能够在小麦根际及小麦体表定殖(包括根表和茎叶表面);相对于在茎叶表面定殖的A-47-gf p在根表定殖的菌体与根的结合更为牢固;从根基到根尖A-47-gfp的定殖量有明显的减少趋势。     

2020年我国水稻产业形势分析及2021年展望

在国家政策支持下,2020年我国水稻面积恢复增长至3 007.6万hm2,比2019年增加38.2万hm2;受灾害偏重发生影响,水稻平均单产7 044.0 kg/hm2,降低15.0 kg/hm2,但仍为历史第2高;水稻总产2.1186亿t,增加225.0万t,连续10年稳定在2亿t以上.国内稻米市场价格持续上涨,特别...     

半球面影像技术估测三种园林树种的绿量

利用半球面影像技术获取高山榕、小叶榕、蒲桃3种园林树种的LAI值,结合各个体树冠投影面积(S)和冠高(H),计算并比较3个绿量测算指标(LAI、LAI×S和LAI×S×H).结果表明:LAI是绿量基本的测度单位,而LAI×S能够较合理地反映3种园林树种种间或种内的绿量差异,可以作为一个绿量的评估指标,以此估计3种园林树种的绿量为小叶榕＞高山榕＞蒲桃.LAI×S×H由于参数较多,容易造成误差,且其数据过于密集,不适宜用于绿量分析.随着半球面影像技术等无损性光学测量技术的发展,最终应用LAI估算绿色植物的绿量会是最可靠的方法.     

饲料中添加白术多糖对林下养殖绿壳蛋鸡生产性能与蛋品质的影响

为研究饲料中添加白术多糖对林下养殖绿壳蛋鸡生产性能与蛋品质的影响,试验选用28周龄产蛋的东乡黑羽绿壳蛋鸡540只,随机分为3组,每组3个重复,每个重复60只,1组为对照组饲喂基础日粮,试验2、3组分别在基础日粮中添加2.5%、5.0%白术多糖,预饲期7 d,试验期28 d。在试验期间,测定林下养殖绿壳蛋鸡生产性能和蛋品质。结果表明:(1)试验3组产蛋率和平均蛋重分别较对照组提高7.33%、6.26%(P <0.05);试验3组料蛋比较对照组降低12.64%(P <0.05);试验2、3组的商品合格率、日产蛋量、日均采食量均高于1组,差异不显著(P> 0.05)。(2)试验3组蛋质量、哈夫单位、蛋壳厚度分别较对照组提高5.63%、3.02%、6.12%(P <0.05);试验2、3组的蛋形指数、蛋黄颜色、蛋黄比例均高于1组,差异不显著(P> 0.05)。综上所述,在基础饲料中添加5.0%白术多糖可以提高林下养殖绿壳蛋鸡生产性能与蛋品质。     

沙对高尔夫果岭坪床物理特性的影响

对28种沙与泥炭按75∶25混配而成的高尔夫果岭坪床材料的物理特性进行了评价。不同类型沙的粒径、均一性和形状有所不同。高尔夫果岭坪床的物理特性主要取决于所使用的沙的特性。对沙 泥炭混配的坪床来说,其导水率在95~827 mm/h范围内变化,总孔隙度变化范围为38.8%~47.2%,而通气孔隙度(在-3 kPa水势下)为0.9%~24.6%,毛管孔隙度(在-3 kPa水势下)为17.7%~42.1%。结果表明,排水速率和通气孔隙度与毛管孔隙度的平衡主要受沙的粒径大小的影响,起决定作用的是粒径最大和最小的沙所占的比例,而容重、总孔隙度和抗剪切强度取决于沙的均一性。当沙粒较圆且较为光滑时,坪床材料孔隙度趋向减少。沙表面越粗糙,坪床抗剪切能力就越强,而沙对坪床硬度的作用不显著。     

Green justice in the city: A new agenda for urban green space research in Europe

In this short communication, we discuss European urban green space (UGS) research from an environmental justice perspective. We show that European UGS scholarship primarily focuses on functional values and managerial aspects of UGS, while paying less attention to equity in the enjoyment of and decision-making around UGS. On this basis we discuss potentials for European urban green space research to take up a more explicit environmental justice framing to shed much-needed light on injustices in European cities and inspire change in policy and practice.     

Incorporating local ecological knowledge into urban riparian restoration in a mountainous region of Southwest China

Modern cities use straightened and concrete watercourses with simple greening for riparian zones, which has been criticised for insensitivity to natural system integrity and cultural identity. This increases the challenge to urban ecologists, landscape designers and managers to conceive innovative and effective design solutions that do not jeopardise hydraulic efficiency yet are culturally acceptable to local communities. This paper introduces the field of local ecological knowledge (LEK) as rich sources of inspiration and design solutions to meet this challenge. LEK refers to knowledge and practices of various local cultures about the relationship of living beings (including humans) with their environments. Wepropose a pragmatic framework that integrates LEK with modern landscape design. It includes steps: (1) investigate existing practices, skills andmeasures relating to LEK; (2) explore how and whereLEKcan inspire or integrate withdesign. Here we propose three aspects for integration in accordance with the nature of landscaping process: overall structure, component and maintenance.(3)evaluate and test the integration in terms of its acceptability by local communities. Taking the riparian revegetation project of Hanfeng Lake Urban Wetland Park (Chongqing Municipality) as an example, we illustrate the utility of this framework. 11 local common practices relating to LEK were identified. According to thethree aspects for integration, alternative design solutions were offered. The survey showed that LEK based designwas more highly valued than other conventional ways in terms of useracceptance. We conclude that LEK provides actionable ecologically sound and culturally desirablesolutions for urbanlandscape. We also recognise that LEK evolveswith changing environments and new harmonious and dynamic human-nature relationships arecontinually re-forming. The process of acquisition and application of LEK could encourage multidisciplinary and synthetic approaches to urban ecosystems. Such adaptability and interdisciplinary engagementare crucial totoday’schanging and complexurban environments.     

Influence of tree crown characteristics on the local PM10 distribution inside an urban street canyon in Antwerp (Belgium): A model and experimental approach

Apart from influencing the amount of leaf-deposited particles, tree crown morphology will influence the local distribution of atmospheric particles. Nevertheless, tree crowns are often represented very rudimentary in three-dimensional air quality models. Therefore, the influence of tree crown representation on the local ambient PM₁₀ concentration and resulting leaf-deposited PM₁₀ mass was evaluated, using the three-dimensional computational fluid dynamics (CFD) model ENVI-met^® and ground-based LiDAR imaging. The modelled leaf-deposited PM₁₀ mass was compared to gravimetric results within three different particle size fractions (0.2–3, 3–10 and >10 μm), obtained at 20 locations within the tree crown. Modelling of the LiDAR-derived tree crown resulted in altered atmospheric PM₁₀ concentrations in the vicinity of the tree crown. Although this model study was limited to a single tree and model configuration, our results demonstrate that improving tree crown characteristics (shape, dimensions and LAD) affects the resulting local PM₁₀ distribution in ENVI-met. An accurate tree crown representation seems, therefore, of great importance when aiming at modelling the local PM distribution.     

岷江上游干旱河谷岷江柏木幼林地绿肥种植研究

在岷江上游干旱河谷岷江柏木幼林地经过连续4 a利用雨季种植绿肥压青,增加土壤肥力,提高水分利用率,促进幼树生长研究。结果表明:(1)种植红豆草的土壤0～40 cm含水量旱季增加3.68 g·kg-1,0～20 cm土壤容重降低0.07 g·cm-3;种植草木樨土壤0～40 cm含水量旱季增加4.18 g·kg-1,0～20 cm土壤容重降低0.05 g·cm-3;种植沙打旺土壤0～40 cm含水量增加2.46 g.kg-1,0～20 cm土壤容重降低0.18 g·cm-3。(2)种植绿肥压青,土壤有机质增加0.37 g·kg-1～0.55 g·kg-1,土壤CaCO3含量下降0.66 g·kg-1～1.36 g·kg-1,有效氮增加4.5 mg·kg-1～18.5 mg·kg-1,有效磷含量增加1.0～3.46 mg·kg-1,CEC比对照增加0.54～0.58mg.100g-1。(3)种植红豆草、草木樨、沙打旺绿肥压青对岷江柏木树高、地径生长有显著的促进作用,尤其种植红豆草的岷江柏木幼树生长最好,高、径生长为对照的1.7倍和1.6倍。     

近10年我国小城镇绿地系统研究进展

梳理了近10年来我国学者对小城镇绿地系统的研究成果,对文献研究的状况和内容进行了总结。结果表明,2008年后小城镇绿地系统的建设逐渐成为小城镇建设的热点问题,形成了以风景园林为主的5个学科研究结构、7个研究方向及3个研究层面,但理论深度不够、研究队伍单薄、量化研究不足,以及国家标准缺乏等制约了相关研究的发展。最后,从7个方面提出中国小城镇绿地系统的研究展望。