新形势下我国高等职业教育教学管理体系的构建

本文探索了新形势下我国高等职业技术教育教学管理体系建设方面的一些问题,就师资队伍、实训基地、教材建设及办学形式、办学观念等方面提出了一些建议。     

Contrasting effect of perlecan on adipogenic and osteogenic differentiation of mesenchymal stem cells in vitro

Perlecan, a basement membrane component, shows diverse functions in different organs and tissues. However, the role of perlecan in differentiation of mesenchymal stem cells (MSCs) has been barely investigated. In this study, we examined the effect of perlecan on adipogenic and osteogenic differentiation of MSCs in vitro by adding extrinsic perlecan to culture media or blocking the function of intrinsic perlecan expressed into culture media by differentiating MSCs. Extrinsic perlecan suppressed adipogenic differentiation; however, it promoted osteogenic differentiation. These functions were further confirmed by a study of blocking intrinsic perlecan. Perlecan treated with heparitinase‐I also showed the suppressive effect on adipogenic differentiation. In contrast, the promotive effect on osteogenic differentiation was found to be heparan sulfate‐dependent. Intrinsic perlecan was suggested to be effective at the late stage of adipogenic differentiation by a study of perlecan‐blocking performed at distinct periods, but was suggested to be effective at the early stage of osteogenic differentiation. Our results showed perlecan has contrasting effect on adipogenic and osteogenic differentiation of MSCs due to its diverse actions. Based on these outcomes, we recognized that employing extrinsic perlecan or blocking intrinsic perlecan is effective for regulating adipogenic and osteogenic differentiation of MSCs by restricting its direction.     

浅析阴阳学说在中医临床上的应用

本文主要探讨中医阴阳的相互关系以及其在临床上的体现和应用。着重强调了中医阴阳学说在中医学中的重要性,明确了掌握中医阴阳的关系能更好地帮助人们深入了解和学习中医理论,更有利于在临床上明确诊断、确立治疗原则和指导选方用药。