植物引种驯化研究概述

引种驯化试验是不断充实和加强植物资源基因库,丰富植物品种多样性的一种重要举措。文章正是在阅读大量相关文献的基础上,阐述引种驯化的定义、理论和方法,并对引种驯化的影响因素和引种试验技术进行详细介绍,从而更好的了解引种驯化的全过程,对引种试验的成功开展具有指导作用。     

A Genetic Study of Frost Resistance in Wheat Callus Culture

The genetic control of frost resistance was studied in callus cultures using some of the chromosome substitution lines of the variety ‘Cheyenne’ into ‘Chinese Spring’. The survival of the calli derived from immature embryos was studied with triphenyltet-razolium chloride (TTC) and fluorescein diacetate (5DA) methods after hardening and freezing at a temperatures of -7 °C, -9 °C, -11 °C, -13 °C, and –15 °C. The donor ‘Cheyenne’ and the substitution lines 5A and 5D proved to be more frost resistant than the recipient ‘Chinese Spring1. These results are well correlated with the previously published studies when seedlings were tested under controlled conditions. Based on these results the tissue culture technique seems to be useful for testing varieties and lines for different levels of frost resistance and even for mutant selection.