全文获取类型
收费全文 | 1725篇 |
免费 | 52篇 |
国内免费 | 113篇 |
专业分类
林业 | 87篇 |
农学 | 243篇 |
基础科学 | 40篇 |
121篇 | |
综合类 | 836篇 |
农作物 | 17篇 |
水产渔业 | 1篇 |
畜牧兽医 | 50篇 |
园艺 | 355篇 |
植物保护 | 140篇 |
出版年
2024年 | 9篇 |
2023年 | 25篇 |
2022年 | 52篇 |
2021年 | 45篇 |
2020年 | 51篇 |
2019年 | 47篇 |
2018年 | 55篇 |
2017年 | 46篇 |
2016年 | 72篇 |
2015年 | 69篇 |
2014年 | 90篇 |
2013年 | 95篇 |
2012年 | 132篇 |
2011年 | 146篇 |
2010年 | 139篇 |
2009年 | 122篇 |
2008年 | 96篇 |
2007年 | 96篇 |
2006年 | 95篇 |
2005年 | 55篇 |
2004年 | 59篇 |
2003年 | 58篇 |
2002年 | 44篇 |
2001年 | 38篇 |
2000年 | 18篇 |
1999年 | 21篇 |
1998年 | 18篇 |
1997年 | 15篇 |
1996年 | 7篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 6篇 |
1992年 | 17篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1976年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有1890条查询结果,搜索用时 15 毫秒
101.
基于随机森林算法和气象因子的砀山酥梨始花期预报 总被引:5,自引:4,他引:1
准确预报始花期是制定砀山酥梨花期管理措施和赏花活动方案的重要基础。该文利用1983-2018年砀山酥梨始花期的定位观测物候数据和平行观测的气象资料,采用线性趋势法,揭示始花期演变趋势;采用相关分析,筛选影响始花期的关键气象因子,依据不同预报日期构成特征变量集;采用随机森林算法(Random Forest, RF),自3月11日开始预报到3月25日终止预报,每日训练1个预报模型。结果表明,1)1983-2018年始花期呈极显著提早发生趋势,每10a约提前2.750 d(P<0.001)。2)16个逐日气象预报模型中,共计有200个气象因子与始花期早迟密切相关,相关系数在0.469~0.789之间;各气象预报模型的训练集与测试集的平均正确率(Nd)分别为92.9%和75.5%、平均均方根误差(RMSE)分别为1.693~2.870和2.240~7.237、平均决定系数(R2)分别为0.891和0.701。3)2019年试验预报中,提前15日准确预报出当年始花期。该文研究表明RF在梨树始花期逐日气象预报中有一定业务应用潜力,预报准确率基本满足气象服务需求。 相似文献
102.
103.
有机与无机肥料对山地梨枣品质的影响 总被引:1,自引:0,他引:1
设置不同无机肥料(氮肥、磷肥、钾肥和氮磷钾混合肥施用)、不同有机肥(沼肥和腐熟油渣肥)和不施肥(对照),研究不同施肥方法对梨枣的水分含量,总糖、可溶性固形物、还原型抗坏血酸和总抗坏血酸、质构特性以及有机酸的影响。结果表明:肥料对梨枣的水分含量没有显著性影响,显著降低了梨枣的苹果酸,柠檬酸和有机酸总含量。有机肥沼肥和腐熟油渣肥可显著提高梨枣的果皮果肉硬度和咀嚼性以及可溶性固形物、总糖含量、抗坏血酸含量,酒石酸含量却显著降低。无机肥料氮肥、磷肥、钾肥中,钾肥有利于梨枣的可溶性固形物、总糖、抗坏血酸,苹果酸和柠檬酸的含量积累,而酒石酸含量却减少。表明增施有机肥和钾肥有利于山地梨枣品质的提高。 相似文献
104.
F. Kleitman D. Shtienberg D. Blachinsky D. Oppenheim M. Zilberstaine O. Dror S. Manulis 《Plant pathology》2005,54(2):108-115
Oxolinic acid (OA) has been the only bactericide used against fire blight in pear and quince orchards in Israel since 1998. OA-resistant Erwinia amylovora strains (Ea-OAR) were detected in several orchards in two restricted areas in the northern Galilee region during 1999–2001. In the following years, resistant strains could not be detected in some of these locations. Documenting the fate of Ea-OAR strains in commercial orchards at eight sites in northern Israel during 2000/03 revealed that the resistant population appeared irrespective of the number of sprays applied and the severity of the disease. The persistence of the Ea-OAR populations varied from site to site, ranging from 4 to 20 months; these differences could be attributed to the fire blight management activities of growers. Comparative studies on the fitness of Ea-OAR and E. amylovora strains sensitive to OA (Ea-OAS) were conducted in vitro and in planta using two strains of each group. In four of the six comparisons, disease incidence on detached blossoms inoculated with Ea-OAS was significantly higher than that on blossoms inoculated with Ea-OAR. In two experiments conducted on 8-year-old pear trees grown under netting, the colonization of Ea-OAS in blossoms, annual shoots and perennial spurs was significantly higher than that of the Ea-OAR. In two experiments conducted on 2-year-old trees grown under netting in an experimental station, the incidence of shoots exhibiting fire blight symptoms and the rate of symptom progress within the branches were significantly higher in trees inoculated with Ea-OAS than in those inoculated with Ea-OAR. The results of this study suggest that OA-resistant E. amylovora strains have lower fitness than wild-type strains. These findings may have implications for fire blight management. 相似文献
105.
106.
梨组织中苹果褪绿叶斑病毒的原位RT-PCR检测 总被引:6,自引:2,他引:6
为了建立梨树苹果褪绿叶斑病毒原位RT-PCR检测技术, 用已知带有苹果褪绿叶斑病毒的库尔勒香梨和无病毒实生苗叶片为材料, 利用D IG标记, 研究了香梨病毒的叶片石蜡切片IS-RT-PCR检测技术。包括AMV逆转录酶、dNTPs、RNasin及互补引物浓度对cDNA合成的影响, 退火温度、Taq DNA聚合酶、Mg2+ 、引物浓度及循环次数对原位PCR效果的影响。结果表明: RNasin的用量大于0.2 U·μL-1时,信号强度随着RNasin量的加大而增强; 只有当dNTPs浓度达1.0 mmol·L-1时才能生成一定量的cDNA;AMV浓度在0.3~0.5 U·μL-1均可进行正常的逆转录, 而且在该范围内产物的量随AMV浓度提高而增多; 引物浓度达到0.9μmol·L-1以上时才能进行有效的逆转录, 并且生成的cDNA的量随引物浓度增大而增加。原位扩增ACLSV的cDNA适合退火温度为56℃。循环20~30次可出现较强的蓝色信号, 引物浓度在0.8~1.2μmol·L-1时显色较好; Taq酶浓度为20 U·mL-1以上, 均显示较深的蓝色; Mg2 +浓度为1.5mmol·L-1就可满足原位PCR所需。获得了苹果褪绿叶斑病毒的原位PCR优化检测体系, 利用建立的优化程序对香梨样品进行了检测验证, 取得了很好效果。 相似文献
107.
108.
针对鞍山海城地区的南果梨和沈阳苏家屯地区的苹果梨初果期和盛果期适宜采收期的生理生化指标进行了详细调查,结果表明:南果梨在海城地区初果期和盛果期最适采收期分别在9月20日、9月15日左右,一级果主要生理指标是:种子变黑指数分别是96.7、95.2,可溶性固形物是16.6%、16.1%,果实自然落果率分别是1.89%、1.26%。苹果梨在苏家屯地区初果期和盛果期最适采收期均在9月30日左右,一级果主要生理指标是:种子变黑指数分别是95.7、96.1,可溶性固形物分别是13.1%、13.4%,果实自然落果率分别是1.98%、2.38%。 相似文献
109.
To obtain the basic information on fruit set regulation, effects of several RNases including S-RNase on pollen tube growth and RNA degradation in the tube were studied in the pear. Purified S-RNase from the Japanese pear ‘Kosui’ (S4S5) predominantly inhibited the growth of ‘Kosui’ pollen tubes (self) in vitro at 0.28 unit μL−1, but it inhibited ‘Chojuro’ (S2S3) pollen (cross) only slightly. The same unit of RNase T1 (EC 3.1.27.3) clearly inhibited the pollen tube growth, but the action was significantly weaker than that of the S-RNase against the self-pollen. Inhibitory effect of RNase T2 (EC 3.1.27.1) and RNase A (EC 3.1.27.5) was only slight. The proteins other than the S-RNase extracted from pear style did not have any inhibitory action, though they possessed RNase activity 3.8 times higher than S-RNase. Thus, RNases tested here could not substitute for the S-RNase in specific inhibition against the self-pollen tube growth. Total RNA degradation by each RNase occurred in the pollen tubes as following order; S-RNase (self) ≥T1 > T2 ≥ A > S-RNase (cross). Degradation degree of 28S and 18S rRNA was as follows; S-RNase (self) > A > T1 > T2 > S-RNase (cross). The degradation of 5.8S and 5S rRNA was; S-RNase (self) > S-RNase (cross) > A > T2 > T1. The degree of rRNA degradation was, thus, not always in parallel with the degree of pollen growth inhibition. The S-RNase may degrade not only rRNA but also mRNA essential for pollen tube growth, and may be specifically adapted to inhibit the growth of self-pollen tubes. Therefore, controlling S-RNase amount in the style will produce self-thinning cultivars efficiently, which are unnecessary not only for hand-pollination but fruit-thinning practices in the pear. Practically, cultivar with weak self-incompatibility and small amount of S-RNase, such as ‘Okusankichi’, may be an expecting candidate for breeding self-thinning cultivars. 相似文献
110.
Proliferation and collapse of subcuticular hyphae of Venturia nashicola race 1 were studied ultrastructurally, after inoculation of susceptible Japanese pear cv. Kousui, resistant Japanese pear
cv. Kinchaku, resistant Asian pear strain Mamenashi 12 and nonhost European pear cv. Flemish Beauty leaves, to understand
the nature of the resistance mechanism. After cuticle penetration by the pathogen, the hyphae were observed at lower frequency
in epidermal pectin layers and middle lamellae of leaves of the three resistant plants than in those of susceptible ones.
This result suggested that fungal growth was suppressed in the incompatible interaction between pear and V. nashicola race 1. In the pectin layers of all inoculated plants, some hyphae had modifications such as breaks in the plasmalemma with
plasmolysis, necrotic cytoplasm and degraded cell walls. More hyphae had collapsed in the leaves of the three resistant plants
than in those of the susceptible cv. Kousui. In collapsed hyphae, the polymerized cell walls broke into numerous fibrous and
amorphous pieces, showing that the scab resistance might be associated with cell wall-degrading enzymes from pear plants. 相似文献